pCMV-3×FLAG-miniTurboID-13X GG

pCMV-3×FLAG-miniTurboID-13X GGGGS-EBF1-NLS-Neo plasmid

PVT94121

pCMV-3×FLAG-miniTurboID-13X GGGGS-EBF1-NLS-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-miniTurboID-13X GGGGS-EBF1(human)-NLS-Neo
Alias: NM_024007.5;COE1; EBF; O/E-1; OLF1
Gene lenght: 1773bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: BGH-R
Copy number: High
Induction:

pCMV-3×FLAG-miniTurboID-13X GGGGS-EBF1-NLS-Neo plasmid Description

pCMV-3×FLAG-miniTurboID-13X GGGGS-EBF1-NLS-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-miniTurboID-13X GGGGS-EBF1-NLS-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt ATCCCCCTGC TGAACGCCAA ACAGATCCTG 1021 GGCCAGCTGG ACGGCGGCTC CGTGGCTGTT CTGCCCGTGG TGGATAGCAC CAACCAGTAC 1081 CTGCTGGATA GAATTGGCGA GCTGAAGAGC GGCGATGCCT GCATCGCCGA GTATCAGCAG 1141 GCCGGCAGGG GCAGCAGGGG CAGGAAATGG TTCAGCCCCT TCGGCGCCAA CCTGTATCTG 1201 TCCATGTTCT GGAGGCTGAA AAGGGGCCCT GCCGCCATCG GCCTGGGCCC TGTGATCGGA 1261 ATCGTGATGG CCGAGGCCCT GAGAAAACTG GGCGCCGACA AGGTGAGGGT GAAATGGCCC 1321 AACGACCTGT ACCTGCAGGA TAGGAAGCTG GCCGGCATCC TGGTGGAGCT GGCCGGAATC 1381 ACCGGCGACG CCGCTCAGAT CGTGATCGGC GCCGGCATCA ACGTGGCCAT GAGGAGGGTG 1441 GAGGAGAGCG TGGTGAATCA GGGCTGGATC ACCCTGCAGG AGGCCGGCAT CAATCTGGAC 1501 AGAAATACCC TGGCCGCCAT GCTGATCAGA GAGCTGAGGG CCGCCCTGGA GCTGTTCGAG 1561 CAGGAGGGCC TGGCCCCCTA CCTGTCCAGG TGGGAGAAGC TGGATAACTT CATCAACAGG 1621 CCTGTGAAGC TGATCATTGG CGACAAGGAG ATATTCGGCA TTAGCAGAGG CATCGATAAA 1681 CAGGGCGCCC TGCTGCTGGA GCAGGACGGA GTGATCAAGC CCTGGATGGG CGGCGAGATC 1741 AGCCTGAGAA GCGCCGAGAA GGGTGGAGGC GGGTCTGGAG GCGGGGGTAG TGGCGGGGGT 1801 GGAAGCGGGG GTGGAGGCGG GTCGGGTGGC GGAGGTAGCG GAGGCGGTGG AAGTGGAGGC 1861 GGTGGAAGTG GTGGAGGAGG TTCTGGTGGC GGAGGTAGCG GTGGCGGAGG TAGCGGAGGC 1921 GGTGGAAGTG GTGGCGGAGG TAGCGGAGGC GGTGGATCGg aattcATGTT TGGGATTCAG 1981 GAAAGCATCC AACGGAGTGG AAGCAGCATG AAGGAAGAGC CGCTGGGCAG CGGCATGAAC 2041 GCGGTGCGGA CGTGGATGCA GGGCGCCGGG GTGCTGGACG CCAACACGGC GGCGCAGAGC 2101 GGGGTGGGTC TGGCCCGGGC TCACTTTGAG AAGCAGCCGC CTTCCAATCT GCGGAAATCC 2161 AACTTCTTCC ACTTCGTCCT GGCCCTCTAC GACAGACAGG GCCAGCCCGT GGAGATCGAG 2221 AGGACAGCGT TTGTGGGGTT CGTGGAGAAG GAAAAAGAAG CCAACAGCGA AAAGACCAAT 2281 AACGGAATTC ACTACCGGCT TCAGCTTCTC TACAGCAATG GGATAAGGAC GGAGCAGGAT 2341 TTCTACGTGC GCCTCATTGA CTCCATGACA AAACAAGCCA TAGTGTATGA AGGCCAAGAC 2401 AAGAACCCAG AAATGTGCCG AGTCTTGCTC ACACATGAGA TCATGTGCAG CCGCTGTTGT 2461 GACAAGAAAA GCTGTGGCAA CCGAAATGAG ACTCCCTCAG ATCCAGTGAT AATTGACAGG 2521 TTCTTCTTGA AATTTTTCCT CAAATGTAAC CAAAATTGCC TAAAGAATGC GGGAAACCCA 2581 CGTGACATGC GGAGATTCCA GGTCGTGGTG TCTACGACAG TCAATGTGGA TGGCCATGTC 2641 CTGGCAGTCT CTGATAACAT GTTTGTCCAT AATAATTCCA AGCATGGGCG GAGGGCTCGG 2701 AGGCTTGACC CCTCGGAAGG TACGCCCTCT TATCTGGAAC ATGCTACTCC CTGTATCAAA 2761 GCCATCAGCC CGAGTGAAGG ATGGACGACG GGAGGTGCGA CTGTGATCAT CATAGGGGAC 2821 AATTTCTTTG ATGGGTTACA GGTCATATTC GGTACCATGC TGGTCTGGAG TGAGTTGATC 2881 ACTCCTCATG CCATCCGTGT GCAGACCCCT CCTCGGCACA TCCCTGGTGT TGTGGAAGTC 2941 ACACTGTCCT ACAAATCTAA GCAGTTCTGC AAAGGAACAC CAGGCAGATT CATTTATACA 3001 GCGCTCAACG AACCCACCAT CGATTATGGT TTCCAGAGGT TACAGAAGGT CATTCCTCGG 3061 CACCCTGGTG ACCCTGAGCG TTTGCCAAAG GAAGTAATAC TCAAAAGGGC TGCGGATCTG 3121 GTAGAAGCAC TGTATGGGAT GCCACACAAC AACCAGGAAA TCATTCTGAA GAGAGCGGCC 3181 GACATTGCCG AGGCCCTGTA CAGTGTTCCC CGCAACCACA ACCAACTCCC GGCCCTTGCT 3241 AACACCTCGG TCCACGCAGG GATGATGGGC GTGAATTCGT TCAGTGGACA ACTGGCCGTG 3301 AATGTCTCCG AGGCATCACA AGCCACCAAT CAGGGTTTCA CCCGCAACTC AAGCAGCGTA 3361 TCACCACACG GGTACGTGCC GAGCACCACT CCCCAGCAGA CCAACTATAA CTCCGTCACC 3421 ACGAGCATGA ACGGATACGG CTCTGCCGCA ATGTCCAATT TGGGCGGCTC CCCCACCTTC 3481 CTCAACGGCT CAGCTGCCAA CTCCCCCTAT GCCATAGTGC CATCCAGCCC CACCATGGCC 3541 TCCTCCACAA GCCTCCCCTC CAACTGCAGC AGCTCCTCGG GCATCTTCTC CTTCTCACCA 3601 GCCAACATGG TCTCAGCCGT GAAACAGAAG AGTGCTTTCG CACCAGTCGT CAGACCCCAG 3661 ACCTCCCCAC CTCCCACCTG CACCAGCACC AACGGGAACA GCCTGCAAGC GATATCTGGC 3721 ATGATTGTTC CTCCTATGct cgagccaaag aagaagcgga aggtctaatc tagagggccc 3781 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 3841 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 3901 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 3961 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 4021 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg 4081 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 4141 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 4201 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 4261 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 4321 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 4381 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 4441 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 4501 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 4561 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 4621 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 4681 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 4741 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 4801 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag 4861 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg 4921 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 4981 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 5041 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg 5101 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 5161 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 5221 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 5281 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 5341 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 5401 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg 5461 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 5521 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 5581 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 5641 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 5701 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc 5761 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg 5821 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc 5881 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 5941 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 6001 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct 6061 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 6121 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 6181 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 6241 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 6301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 6421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 6481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 6541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 6601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 6661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 6721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 6781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 6841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 6901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 6961 atccggcaaa caaaccaccg ctggtagcgg tGGTtttttt gtttgcaagc agcagattac 7021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 7081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 7141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 7201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 7261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 7321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 7381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 7441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 7501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 7561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 7621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 7681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 7741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 7801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 7861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 7921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 7981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 8041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 8101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 8161 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.