pLV3-CMV-RAMP1-EF1a-mCherry-Hy

pLV3-CMV-RAMP1-EF1a-mCherry-Hyg plasmid

PVT94116

pLV3-CMV-RAMP1-EF1a-mCherry-Hyg plasmid Information

Plasmid name: pLV3-CMV-RAMP1(human)-EF1a-mCherry-Hyg
Alias: NM_005855.4
Gene lenght: 447bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-RAMP1-EF1a-mCherry-Hyg plasmid Description

pLV3-CMV-RAMP1-EF1a-mCherry-Hyg is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-RAMP1-EF1a-mCherry-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgaat ttaaatcgga tccGCCACCa tggcccgggc cctgtgccgc 2341 ctcccgcggc gcggcctctg gctgctcctg gcccatcacc tcttcatgac cactgcctgc 2401 caggaggcta actacggtgc cctcctccgg gagctctgcc tcacccagtt ccaggtagac 2461 atggaggccg tcggggagac gctgtggtgt gactggggca ggaccatcag gagctacagg 2521 gagctggccg actgcacctg gcacatggcg gagaagctgg gctgcttctg gcccaatgca 2581 gaggtggaca ggttcttcct ggcagtgcat ggccgctact tcaggagctg ccccatctca 2641 ggcagggccg tgcgggaccc gcccggcagc atcctctacc ccttcatcgt ggtccccatc 2701 acggtgaccc tgctggtgac ggcactggtg gtctggcaga gcaagcgcac tgagggcatt 2761 gtgtaggcgg ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac 2821 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag 2881 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga 2941 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg 3001 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg 3061 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg 3121 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg 3181 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct 3241 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc 3301 caagctgtga ccggcgccta cgctagacgc caccatggtg agcaagggcg aggaggataa 3361 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 3421 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 3481 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 3541 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3601 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3661 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct 3721 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga 3781 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3841 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 3901 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 3961 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 4021 cggcatggac gagctgtaca agggatctcg cgagggcaga ggaagtcttc taacatgcgg 4081 tgacgtggag gagaatcccg gccctGCCAC Catggctgaa caagatggat tgcacgcagg 4141 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 4201 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 4261 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct 4321 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 4381 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 4441 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 4501 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 4561 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 4621 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 4681 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 4741 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 4801 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 4861 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaGTCG ACAATCAACC 4921 TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC 4981 GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT 5041 CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT 5101 TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG 5161 CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC 5221 GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC 5281 TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT 5341 TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC 5401 GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG 5461 CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG Cggtaccttt aagaccaatg 5521 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg 5581 ctaattcact cccaacgaaG ataagatctg ctttttgctt gtactgggtc tctctggtta 5641 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 5701 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 5761 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 5821 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac 5881 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 5941 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 6001 catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 6061 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 6121 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt 6181 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 6241 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 6301 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 6361 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 6421 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 6481 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 6541 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 6601 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 6661 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 6721 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 6781 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 6841 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 6901 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 6961 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 7021 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 7081 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 7141 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 7201 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 7261 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 7321 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 7381 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 7441 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 7501 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 7561 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 7621 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 7681 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 7741 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 7801 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 7861 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 7921 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 7981 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 8041 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 8101 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 8161 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 8221 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 8281 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 8341 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga 8401 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 8461 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 8521 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg 8581 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 8641 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 8701 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 8761 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.