pLV3-CMV-MCS-EF1a-mCherry-Hyg

pLV3-CMV-MCS-EF1a-mCherry-Hyg plasmid

PVT94064

pLV3-CMV-MCS-EF1a-mCherry-Hyg plasmid Information

Plasmid name: pLV3-CMV-MCS-EF1a-mCherry-Hyg
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MCS-EF1a-mCherry-Hyg plasmid Description

pLV3-CMV-MCS-EF1a-mCherry-Hyg is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-MCS-EF1a-mCherry-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcC TCGAGGATAT CGGGCCCtga ggatccgcgg ccgcGaagga tctgcgatcg 2341 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg 2401 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga 2461 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt 2521 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg 2581 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg 2641 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta 2701 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc 2761 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt 2821 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc 2881 caccATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT 2941 CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA 3001 GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT 3061 GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG CCTACGTGAA 3121 GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA 3181 GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA 3241 GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC 3301 CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA 3361 CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA 3421 CGCTGAGGTC AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA 3481 CGTCAACATC AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACAGTA 3541 CGAACGCGCC GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA AGGTCGAGGG 3601 AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA GACGTGGAGG AGAACCCTGG 3661 ACCTGTCGAC atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga 3721 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt 3781 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt 3841 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt 3901 gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg 3961 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga 4021 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg 4081 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc 4141 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc 4201 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc 4261 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg 4321 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc 4381 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc 4441 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag 4501 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt 4561 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg 4621 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc 4681 gagggcaaag gaatagTCTA GAaatcaacc tctggattac aaaatttgtg aaagattgac 4741 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 4801 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 4861 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 4921 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 4981 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 5041 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaATC 5101 ATCGTCCTTT CCTtggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 5161 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 5221 tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttgggc 5281 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 5341 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc 5401 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 5461 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 5521 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 5581 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 5641 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 5701 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 5761 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 5821 aactccgccA TCCCGCCCTA ACTCCGCcag ttccgcccat tctccgcccc atggctgact 5881 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 5941 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg 6001 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 6061 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 6121 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 6181 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 6241 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 6301 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 6361 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 6421 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 6481 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 6541 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 6601 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 6661 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 6721 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 6781 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 6841 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 6901 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 6961 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 7021 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 7081 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 7141 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 7201 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 7261 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 7321 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 7381 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 7441 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 7501 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 7561 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 7621 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 7681 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 7741 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 7801 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 7861 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 7921 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 7981 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 8041 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 8101 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 8161 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 8221 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 8281 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 8341 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 8401 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 8461 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 8521 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 8581 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.