pLV3-CMV-IPO7-3×FLAG-CopGFP-Pu

pLV3-CMV-IPO7-3×FLAG-CopGFP-Puro plasmid

PVT94109

pLV3-CMV-IPO7-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-IPO7(human-toxic-opt)-3×FLAG-CopGFP-Puro
Alias: NM_006391.3;Imp7; RANBP7;
Gene lenght: 3114bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-IPO7-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-IPO7-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-IPO7-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg accccaacac cattatcgag 2341 gccctgcggg gcactatgga cccagccctg cgtgaggccg cggagcgcca gctcaatgaa 2401 gcacacaagt ctctgaattt tgtctcaaca ctgctccaga ttactatgtc ggaacagctg 2461 gatttacctg tgagacaggc aggtgttatc tatctgaaaa atatgataac acagtattgg 2521 cctgatcgag aaacagcacc aggggatata tccccttata ctattccaga agaagatcgc 2581 cattgtattc gagaaaatat tgtagaagcc attatccatt ctcctgagct catcagggta 2641 cagcttacta catgcattca tcacatcatc aaacatgatt atccaagccg ctggactgcc 2701 attgtggaca aaattggctt ttatcttcag tccgataaca gtgcttgttg gctaggaatt 2761 cttctttgcc tttatcagct tgtgaaaaat tatgagtata aaaaaccaga ggagcggagt 2821 ccattggtag cagcaatgca gcattttctg ccagttctaa aggatcgttt tatccagctt 2881 ctttctgacc agtctgatca gtctgtcctc atccagaaac agatattcaa gatcttctat 2941 gctcttgttc agtatacact accactggaa ctgataaacc aacagaacct gacagaatgg 3001 atagaaattt taaagactgt tgtgaacagg gatgtaccta atgaaacact tcaagttgaa 3061 gaagatgatc gacctgagtt accatggtgg aaatgcaaga agtgggcctt acatatttta 3121 gcaagacttt ttgaaagata tggaagccct ggcaatgttt ccaaggagta taatgaattt 3181 gctgaagtat ttctgaaggc atttgctgtt ggtgtccagc aagttttatt gaaggtgtta 3241 tatcagtaca aggagaagca atatatggct cctcgagttt tacaacagac attaaattat 3301 attaatcaag gagtttctca tgctctcacc tggaagaatc tgaagcccca tatacaaggc 3361 attatccaag atgttatttt tccattgatg tgctatacag atgctgatga ggaactttgg 3421 caagaagacc cttacgaata tatacgcatg aagtttgatg tgtttgaaga tttcatttct 3481 cctaccactg ctgcccagac acttttgttt acagcctgta gtaagaggaa agaggtactg 3541 caaaagacta tgggattttg ttaccagatt cttacagaac caaatgctga ccctcgaaaa 3601 aaagatggag ccctgcatat gattggctct ttagctgaaa tacttctgaa gaaaaagatc 3661 tataaagatc agatggaata catgttgcag aatcatgtat tccctctctt cagcagtgaa 3721 ctaggctaca tgagagcaag ggcttgctgg gtacttcact atttttgtga agtgaagttc 3781 aaaagtgatc agaaccttca aacagcctta gagctaacaa gaagatgtct gattgatgat 3841 agagaaatgc ctgtgaaagt ggaagctgcc attgcccttc aagtattgat cagcaatcaa 3901 gaaaaagcta aagaatatat cacaccattc atcagacctg taatgcaggc tcttcttcac 3961 attataagag aaacagaaaa tgatgacctt accaatgtaa ttcagaaaat gatctgtgaa 4021 tatagtgaag aagttactcc tattgcagta gaaatgacac aacatttggc aatgacattt 4081 aaccaagtaa tccagacggg gccagatgaa gaaggtagtg atgacaaagc agttactgct 4141 atgggaattc tgaatacaat tgatacactt cttagtgtag ttgaagatca taaagagata 4201 acccaacagc ttgagggaat ctgcttacag gtcattggta ctgttttaca acagcatgtc 4261 ttagaattct atgaggagat cttctcttta gcgcacagtt tgacatgtca acaagtgtct 4321 ccacagatgt ggcagctact accccttgta tttgaagtct ttcagcaaga tggctttgat 4381 tactttacag atatgATGCC ACTGCTCCAT AATTACGTGA CCGTCGATAC CGATACCCTG 4441 CTTAGCGACA CGAAGTACCT CGAGATGATT TATTCAATGT GCAAAAAGGT GCTGACTGGC 4501 GTCGCAGGTG AGGACGCAGA GTGTCATGCA GCCAAACTGC TGGAAGTGAT CATTCTGCAA 4561 TGCAAAGGCA GAGGGATTGA TCAGTGTATC CCTCTGTTTG TCGAGGCTGC TCTGGAACGA 4621 CTGACCAGAG AGGTGAAGAC ATCAGAACTT CGCACTATGT GTCTCCAAGT CGCCATTGCA 4681 GCTTTGTACT ACAACCCGCA TCTGCTGCTG AATACTCTTG AAAATTTGCG GTTTCCAAAT 4741 AACGTCGAGC CCGTTACCAA CCATTTCATA ACTCAGTGGC TTAACGACGT AGACTGCTTC 4801 CTGGGACTGC ATGATAGGAA AATGTGTGTG TTGGGCCTCT GTGCCCTGAT TGACATGGAA 4861 CAGATTCCCC AGGTTCTGAA CCAGGTTAGT GGGCAGATTT TGCCGGCCTT CATACTGCTG 4921 TTCAATGGAC TGAAAAGAGC CTATGCTTGC CATGCCGAAC ACGAAAACGA CTCCGACGAC 4981 GACGATGAAG CAGAAGACGA TGATGAAACT GAGGAGCTCG GGTCCGATGA AGACGATATT 5041 GACGAGGATG GACAGGAATA CCTGGAGATC CTGGCCAAAC AGGCCGGTGA GGACGGTGAC 5101 GACGAAGATT GGGAAGAGGA TGATGCAGAG GAGACTGCTC TGGAAGGCTA TTCTACAATC 5161 ATCGACGATG AAGACAACCC AGTGGACGAG TATCAGATAT TCAAGGCCAT CTTCCAGACA 5221 ATTCAGAACC GCAATCCCGT ATGGTATCAG GCACTGACTC ACGGGCTGAA TGAGGAACAA 5281 CGCAAACAGC TCCAAGACAT CGCCACACTC GCCGACCAGA GAAGAGCCGC GCACGAAAGC 5341 AAGATGATCG AAAAGCACGG AGGGTATAAA TTCTCAGCGC CCGTTGTGCC TAGCTCCTTT 5401 AATTTCGGGG GTCCAGCACC AGGCATGAAC ggatccGACT ACAAAGACCA TGACGGTGAT 5461 TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGtagcggcc gcGaaggatc 5521 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 5581 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 5641 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 5701 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 5761 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 5821 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 5881 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 5941 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 6001 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 6061 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 6121 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 6181 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 6241 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 6301 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 6361 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 6421 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 6481 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 6541 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 6601 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 6661 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 6721 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 6781 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 6841 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 6901 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 6961 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 7021 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 7081 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 7141 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 7201 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 7261 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 7321 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 7381 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 7441 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac 7501 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 7561 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 7621 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 7681 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 7741 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 7801 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 7861 gtggtgttgt cggggaaATC ATCGTCCTTT CCTtggctgc tcgcctgtgt tgccacctgg 7921 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 7981 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tTcgccttcg ccctcagacg 8041 agtcggatct ccctttggGc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 8101 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 8161 ctcccaacga aGataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 8221 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 8281 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 8341 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 8401 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 8461 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 8521 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 8581 gctctagcta TCCCGCCCCT AACTCCGCCC Atcccgcccc taactccgcc cagttccgcc 8641 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 8701 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 8761 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat 8821 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 8881 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 8941 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 9001 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 9061 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 9121 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 9181 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 9241 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 9301 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 9361 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 9421 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 9481 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 9541 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 9601 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 9661 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 9721 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 9781 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 9841 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 9901 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 9961 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 10021 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 10081 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 10141 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 10201 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 10261 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 10321 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 10381 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 10441 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 10501 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 10561 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 10621 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 10681 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 10741 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 10801 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 10861 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 10921 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 10981 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 11041 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 11101 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 11161 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 11221 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 11281 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 11341 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 11401 gctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.