pLV3-EF1a-MCS-P2A-copGFP-PGK-P

pLV3-EF1a-MCS-P2A-copGFP-PGK-Puro plasmid

PVT91206

pLV3-EF1a-MCS-P2A-copGFP-PGK-Puro plasmid Information

Plasmid name: pLV3-EF1a-MCS-P2A-copGFP-PGK-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-MCS-P2A-copGFP-PGK-Puro plasmid Description

pLV3-EF1a-MCS-P2A-copGFP-PGK-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-MCS-P2A-copGFP-PGK-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cATCTCGAGg gaagcggagc tactaacttc 2521 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatgga gagcgacgag 2581 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 2641 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 2701 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 2761 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 2821 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 2881 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 2941 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 3001 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 3061 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 3121 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 3181 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 3241 attgccttcg cctagcggcc gctgggtagg ggaggcgctt ttcccaaggc agtctggagc 3301 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac 3361 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc 3421 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag 3481 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga 3541 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt 3601 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg 3661 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac 3721 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt 3781 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 3841 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 3901 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 3961 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 4021 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 4081 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 4141 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 4201 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 4261 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 4321 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 4381 tgagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 4441 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 4501 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 4561 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 4621 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 4681 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 4741 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct 4801 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 4861 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 4921 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 4981 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 5041 aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg 5101 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 5161 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 5221 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 5281 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 5341 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 5401 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 5461 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt 5521 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 5581 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt 5641 tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 5701 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 5761 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 5821 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 5881 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 5941 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 6001 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 6061 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 6121 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 6181 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 6241 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 6301 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 6361 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 6421 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 6481 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 6541 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 6601 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 6661 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 6721 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 6781 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 6841 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 6901 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 6961 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 7021 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 7081 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 7141 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 7201 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 7261 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 7321 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 7381 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 7441 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 7501 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 7561 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 7621 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 7681 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 7741 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 7801 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 7861 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac 7921 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 7981 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat 8041 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga 8101 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg 8161 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 8221 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 8281 gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.