pLV3-CMV-ZDHHC9-3×HA-mCherry-P

pLV3-CMV-ZDHHC9-3×HA-mCherry-Puro plasmid

PVT95169

pLV3-CMV-ZDHHC9-3×HA-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-ZDHHC9(human)-3×HA-mCherry-Puro
Alias: NM_016032.4;CGI89; CXorf11; DHHC9; MMSA1; MRXSR; MRXSZ;ZDHHC10; ZNF379; ZNF380
Gene lenght: 1092bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ZDHHC9-3×HA-mCherry-Puro plasmid Description

pLV3-CMV-ZDHHC9-3×HA-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-ZDHHC9-3×HA-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgtctgt gatggtggtg agaaagaagg tgacacggaa 2341 atgggagaaa ctcccaggca ggaacacctt ttgctgtgat ggccgcgtca tgatggcccg 2401 gcaaaagggc attttctacc tgaccctttt cctcatcctg gggacatgta cactcttctt 2461 cgcctttgag tgccgctacc tggctgttca gctgtctcct gccatccctg tatttgctgc 2521 catgctcttc cttttctcca tggctacact gttgaggacc agcttcagtg accctggagt 2581 gattcctcgg gcgctaccag atgaagcagc tttcatagaa atggagatag aagctaccaa 2641 tggtgcggtg ccccagggcc agcgaccacc gcctcgtatc aagaatttcc agataaacaa 2701 ccagattgtg aaactgaaat actgttacac atgcaagatc ttccggcctc cccgggcctc 2761 ccattgcagc atctgtgaca actgtgtgga gcgcttcgac catcactgcc cctgggtggg 2821 gaattgtgtt ggaaagagga actaccgcta cttctacctc ttcatccttt ctctctccct 2881 cctcacaatc tatgtcttcg ccttcaacat cgtctatgtg gccctcaaat ctttgaaaat 2941 tggcttcttg gagacattga aagaaactcc tggaactgtt ctagaagtcc tcatttgctt 3001 ctttacactc tggtccgtcg tgggactgac tggatttcat actttcctcg tggctctcaa 3061 ccagacaacc aatgaagaca tcaaaggatc atggacaggg aagaatcgcg tccagaatcc 3121 ctacagccat ggcaatattg tgaagaactg ctgtgaagtg ctgtgtggcc ccttgccccc 3181 cagtgtgctg gatcgaaggg gtattttgcc actggaggaa agtggaagtc gacctcccag 3241 tactcaagag accagtagca gcctcttgcc acagagccca gcccccacag aacacctgaa 3301 ctcaaatgag atgccggagg acagcagcac tcccgaagag atgccacctc cagagccccc 3361 agagccacca caggaggcag ctgaagctga gaagggatcc TACCCATACG ACGTACCTGA 3421 TTATGCAACT TATCCTTATG ATGTACCTGA TTATGCTAGC TATCCTTATG ATGTCCCAGA 3481 CTACGCATAG gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag tgggcagagc 3541 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct 3601 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc 3661 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 3721 acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg 3781 cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg 3841 cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac 3901 cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt 3961 gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca 4021 gatccaagct gtgaccggcg cctacgctag acgccaccat ggtgagcaag ggcgaggagg 4081 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 4141 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 4201 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 4261 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 4321 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 4381 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga 4441 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 4501 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 4561 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 4621 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 4681 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 4741 ccggcggcat ggacgagctg tacaagggat ctcgcgaggg cagaggaagt cttctaacat 4801 gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg 4861 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc 4921 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag 4981 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg 5041 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga 5101 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag 5161 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct 5221 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg 5281 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct 5341 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct 5401 ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa 5461 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 5521 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 5581 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 5641 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 5701 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 5761 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 5821 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 5881 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 5941 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc 6001 tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc 6061 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat 6121 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 6181 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 6241 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 6301 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 6361 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg 6421 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 6481 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc 6541 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 6601 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 6661 ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt 6721 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 6781 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 6841 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 6901 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 6961 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 7021 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 7081 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7141 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7201 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 7261 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 7321 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7381 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7441 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7501 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 7561 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7621 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7681 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7741 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 7801 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 7861 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 7921 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 7981 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 8041 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 8101 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 8161 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 8221 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 8281 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 8341 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 8401 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 8461 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 8521 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 8581 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 8641 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 8701 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 8761 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 8821 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 8881 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 8941 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 9001 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 9061 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 9121 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 9181 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 9241 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 9301 acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.