pU6-ZDHHC18-shRNA1-EGFP-Puro p

pU6-ZDHHC18-shRNA1-EGFP-Puro plasmid

PVT94099

pU6-ZDHHC18-shRNA1-EGFP-Puro plasmid Information

Plasmid name: pU6-ZDHHC18(human)-shRNA1-EGFP-Puro
Alias: GCTGCCATCCTCTTCTTCTTC
Gene lenght:
Host: Mammalian cells
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pU6-ZDHHC18-shRNA1-EGFP-Puro plasmid Description

pU6-ZDHHC18-shRNA1-EGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells.

pU6-ZDHHC18-shRNA1-EGFP-Puro plasmid Sequence

1 GAGGGCCTAT TTCCCATGAT TCCTTCATAT TTGCATATAC GATACAAGGC TGTTAGAGAG 61 ATAATTAGAA TTAATTTGAC TGTAAACACA AAGATATTAG TACAAAATAC GTGACGTAGA 121 AAGTAATAAT TTCTTGGGTA GTTTGCAGTT TTTAAAATTA TGTTTTAAAA TGGACTATCA 181 TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 241 ACGAAACACC GTGTCTTCCT CGAGGCTGCC ATCCTCTTCT TCTTCttcaa gagaGAAGAA 301 GAAGAGGATG GCAGCttttt tAAGCTTGAT ATCGAATTCC TGCAGCCCGG GGGATCCACT 361 AGTTCTAGAG CGGCCGCCAC CGCGGTGGAG CTCCAGCTTT TGTTCCCTTT AGTGAGGGTT 421 AATTGCGCGT TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA 481 AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC 541 AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG 601 TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCAG 661 TTATCTAGAT CCGGTGGATC TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT 721 CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT 781 GCTCAGGGCG GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC 841 GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG 901 GCGGATCTTG AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT 961 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC 1021 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG 1081 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG 1141 CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG 1201 CACGCCGTAG GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT 1261 GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC 1321 GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT 1381 GAACAGCTCC TCGCCCTTGC TCACCATGGT GGCGACCGGT AGCGCTAGCG GATCTGACGG 1441 TTCACTAAAC CAGCTCTGCT TATATAGACC TCCCACCGTA CACGCCTACC GCCCATTTGC 1501 GTCAATGGGG CGGAGTTGTT ACGACATTTT GGAAAGTCCC GTTGATTTTG GTGCCAAAAC 1561 AAACTCCCAT TGACGTCAAT GGGGTGGAGA CTTGGAAATC CCCGTGAGTC AAACCGCTAT 1621 CCACGCCCAT TGATGTACTG CCAAAACCGC ATCACCATGG TAATAGCGAT GACTAATACG 1681 TAGATGTACT GCCAAGTAGG AAAGTCCCAT AAGGTCATGT ACTGGGCATA ATGCCAGGCG 1741 GGCCATTTAC CGTCATTGAC GTCAATAGGG GGCGTACTTG GCATATGATA CACTTGATGT 1801 ACTGCCAAGT GGGCAGTTTA CCGTAAATAC TCCACCCATT GACGTCAATG GAAAGTCCCT 1861 ATTGGCGTTA CTATGGGAAC ATACGTCATT ATTGACGTCA ATGGGCGGGG GTCGTTGGGC 1921 GGTCAGCCAG GCGGGCCATT TACCGTAAGT TATGTAACGC GGAACTCCAT ATATGGGCTA 1981 TGAACTAATG ACCCCGTAAT TGATTACTAT TAATAACTAA TGCATGGCGG TAATACGGTT 2041 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 2101 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 2161 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 2221 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 2281 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 2341 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 2401 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 2461 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 2521 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT 2581 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 2641 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 2701 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 2761 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 2821 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 2881 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 2941 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 3001 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 3061 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 3121 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 3181 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 3241 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 3301 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 3361 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 3421 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 3481 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 3541 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 3601 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 3661 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 3721 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 3781 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 3841 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTAAATTGT AAGCGTTAAT 3901 ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC 3961 GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT 4021 CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA 4081 ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG 4141 TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA 4201 CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT 4261 AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT 4321 GCGCCGCTAC AGGGCGCGTC CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA 4381 TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA 4441 TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAG 4501 CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCC ACCGCGGTGG CGGCCGCTCT 4561 AGAACTAGTG GATCCCCCGG GCTGCATGGG GTCGTGCGCT CCTTTCGGTC GGGCGCTGCG 4621 GGTCGTGGGG CGGGCGTCAG GCACCGGGCT TGCGGGTCAT GCACCAGGTG CGCGGTCCTT 4681 CGGGCACCTC GACGTCGGCG GTGACGGTGA AGCCGAGCCG CTCGTAGAAG GGGAGGTTGC 4741 GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA CCCCGGCGCG CTCGGCCGCC TCCACTCCGG 4801 GGAGCACGAC GGCGCTGCCC AGACCCTTGC CCTGGTGGTC GGGCGAGACG CCGACGGTGG 4861 CCAGGAACCA CGCGGGCTCC TTGGGCCGGT GCGGCGCCAG GAGGCCTTCC ATCTGTTGCT 4921 GCGCGGCCAG CCGGGAACCG CTCAACTCGG CCATGCGCGG GCCGATCTCG GCGAACACCG 4981 CCCCCGCTTC GACGCTCTCC GGCGTGGTCC AGACCGCCAC CGCGGCGCCG TCGTCCGCGA 5041 CCCACACCTT GCCGATGTCG AGCCCGACGC GCGTGAGGAA GAGTTCTTGC AGCTCGGTGA 5101 CCCGCTCGAT GTGGCGGTCC GGATCGACGG TGTGGCGCGT GGCGGGGTAG TCGGCGAACG 5161 CGGCGGCGAG GGTGCGTACG GCCCTGGGGA CGTCGTCGCG GGTGGCGAGG CGCACCGTGG 5221 GCTTGTACTC GGTCATGGTA AGCTAGCTTG GGCTGCAGGT CGAAAGGCCC GGAGATGAGG 5281 AAGAGGAGAA CAGCGCGGCA GACGTGCGCT TTTGAAGCGT GCAGAATGCC GGGCCTCCGG 5341 AGGACCTTCG GGCGCCCGCC CCGCCCCTGA GCCCGCCCCT GAGCCCGCCC CCGGACCCAC 5401 CCCTTCCCAG CCTCTGAGCC CAGAAAGCGA AGGAGCAAAG CTGCTATTGG CCGCTGCCCC 5461 AAAGGCCTAC CCGCTTCCAT TGCTCAGCGG TGCTGTCCAT CTGCACGAGA CTAGTGAGAC 5521 GTGCTACTTC CATTTGTCAC GTCCTGCACG ACGCGAGCTG CGGGGCGGGG GGGAACTTCC 5581 TGACTAGGGG AGGAGTAGAA GGTGGCGCGA AGGGGCCACC AAAGAACGGA GCCGGTTGGC 5641 GCCTACCGGT GGATGTGGAA TGTGTGCGAG GCCAGAGGCC ACTTGTGTAG CGCCAAGTGC 5701 CCAGCGGGGC TGCTAAAGCG CATGCTCCAG ACTGCCTTGG GAAAAGCGCC TCCCCTACCC 5761 GGTAGAATTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.