pU6-ZDHHC18-shRNA2-EGFP-Puro p

pU6-ZDHHC18-shRNA2-EGFP-Puro plasmid

PVT94010

pU6-ZDHHC18-shRNA2-EGFP-Puro plasmid Information

Plasmid name: pU6-ZDHHC18(human)-shRNA2-EGFP-Puro
Alias: GTTTATTCTCTCCCTCTCATT
Gene lenght:
Host: Mammalian cells
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pU6-ZDHHC18-shRNA2-EGFP-Puro plasmid Description

pU6-ZDHHC18-shRNA2-EGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells.

pU6-ZDHHC18-shRNA2-EGFP-Puro plasmid Sequence

1 GAGGGCCTAT TTCCCATGAT TCCTTCATAT TTGCATATAC GATACAAGGC TGTTAGAGAG 61 ATAATTAGAA TTAATTTGAC TGTAAACACA AAGATATTAG TACAAAATAC GTGACGTAGA 121 AAGTAATAAT TTCTTGGGTA GTTTGCAGTT TTTAAAATTA TGTTTTAAAA TGGACTATCA 181 TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 241 ACGAAACACC GTGTCTTCCT CGAGGTTTAT TCTCTCCCTC TCATTCTCGA GAATGAGAGG 301 GAGAGAATAA ACttttttAA GCTTGATATC GAATTCCTGC AGCCCGGGGG ATCCACTAGT 361 TCTAGAGCGG CCGCCACCGC GGTGGAGCTC CAGCTTTTGT TCCCTTTAGT GAGGGTTAAT 421 TGCGCGTTAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA 481 TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT 541 AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG 601 GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA TGATCAGTTA 661 TCTAGATCCG GTGGATCTGA GTCCGGACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC 721 GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT 781 CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT 841 GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG 901 GATCTTGAAG TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT AGACGTTGTG 961 GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT TGAAGTCGAT 1021 GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT CGGCGCGGGT 1081 CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC CTTCGGGCAT 1141 GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA AGCACTGCAC 1201 GCCGTAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC CGGTGGTGCA 1261 GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT 1321 GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA 1381 CAGCTCCTCG CCCTTGCTCA CCATGGTGGC GACCGGTAGC GCTAGCGGAT CTGACGGTTC 1441 ACTAAACCAG CTCTGCTTAT ATAGACCTCC CACCGTACAC GCCTACCGCC CATTTGCGTC 1501 AATGGGGCGG AGTTGTTACG ACATTTTGGA AAGTCCCGTT GATTTTGGTG CCAAAACAAA 1561 CTCCCATTGA CGTCAATGGG GTGGAGACTT GGAAATCCCC GTGAGTCAAA CCGCTATCCA 1621 CGCCCATTGA TGTACTGCCA AAACCGCATC ACCATGGTAA TAGCGATGAC TAATACGTAG 1681 ATGTACTGCC AAGTAGGAAA GTCCCATAAG GTCATGTACT GGGCATAATG CCAGGCGGGC 1741 CATTTACCGT CATTGACGTC AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT 1801 GCCAAGTGGG CAGTTTACCG TAAATACTCC ACCCATTGAC GTCAATGGAA AGTCCCTATT 1861 GGCGTTACTA TGGGAACATA CGTCATTATT GACGTCAATG GGCGGGGGTC GTTGGGCGGT 1921 CAGCCAGGCG GGCCATTTAC CGTAAGTTAT GTAACGCGGA ACTCCATATA TGGGCTATGA 1981 ACTAATGACC CCGTAATTGA TTACTATTAA TAACTAATGC ATGGCGGTAA TACGGTTATC 2041 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 2101 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 2161 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 2221 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 2281 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 2341 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 2401 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 2461 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 2521 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT 2581 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 2641 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 2701 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 2761 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 2821 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG 2881 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG 2941 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC 3001 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC 3061 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC 3121 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG 3181 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT 3241 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG 3301 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT 3361 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG 3421 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG 3481 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT 3541 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT 3601 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC 3661 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT 3721 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT 3781 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA 3841 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT 3901 TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA 3961 ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA 4021 GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC 4081 GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG 4141 AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG 4201 GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG 4261 GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG 4321 CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG 4381 GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA 4441 AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC 4501 GCGTAATACG ACTCACTATA GGGCGAATTG GAGCTCCACC GCGGTGGCGG CCGCTCTAGA 4561 ACTAGTGGAT CCCCCGGGCT GCATGGGGTC GTGCGCTCCT TTCGGTCGGG CGCTGCGGGT 4621 CGTGGGGCGG GCGTCAGGCA CCGGGCTTGC GGGTCATGCA CCAGGTGCGC GGTCCTTCGG 4681 GCACCTCGAC GTCGGCGGTG ACGGTGAAGC CGAGCCGCTC GTAGAAGGGG AGGTTGCGGG 4741 GCGCGGAGGT CTCCAGGAAG GCGGGCACCC CGGCGCGCTC GGCCGCCTCC ACTCCGGGGA 4801 GCACGACGGC GCTGCCCAGA CCCTTGCCCT GGTGGTCGGG CGAGACGCCG ACGGTGGCCA 4861 GGAACCACGC GGGCTCCTTG GGCCGGTGCG GCGCCAGGAG GCCTTCCATC TGTTGCTGCG 4921 CGGCCAGCCG GGAACCGCTC AACTCGGCCA TGCGCGGGCC GATCTCGGCG AACACCGCCC 4981 CCGCTTCGAC GCTCTCCGGC GTGGTCCAGA CCGCCACCGC GGCGCCGTCG TCCGCGACCC 5041 ACACCTTGCC GATGTCGAGC CCGACGCGCG TGAGGAAGAG TTCTTGCAGC TCGGTGACCC 5101 GCTCGATGTG GCGGTCCGGA TCGACGGTGT GGCGCGTGGC GGGGTAGTCG GCGAACGCGG 5161 CGGCGAGGGT GCGTACGGCC CTGGGGACGT CGTCGCGGGT GGCGAGGCGC ACCGTGGGCT 5221 TGTACTCGGT CATGGTAAGC TAGCTTGGGC TGCAGGTCGA AAGGCCCGGA GATGAGGAAG 5281 AGGAGAACAG CGCGGCAGAC GTGCGCTTTT GAAGCGTGCA GAATGCCGGG CCTCCGGAGG 5341 ACCTTCGGGC GCCCGCCCCG CCCCTGAGCC CGCCCCTGAG CCCGCCCCCG GACCCACCCC 5401 TTCCCAGCCT CTGAGCCCAG AAAGCGAAGG AGCAAAGCTG CTATTGGCCG CTGCCCCAAA 5461 GGCCTACCCG CTTCCATTGC TCAGCGGTGC TGTCCATCTG CACGAGACTA GTGAGACGTG 5521 CTACTTCCAT TTGTCACGTC CTGCACGACG CGAGCTGCGG GGCGGGGGGG AACTTCCTGA 5581 CTAGGGGAGG AGTAGAAGGT GGCGCGAAGG GGCCACCAAA GAACGGAGCC GGTTGGCGCC 5641 TACCGGTGGA TGTGGAATGT GTGCGAGGCC AGAGGCCACT TGTGTAGCGC CAAGTGCCCA 5701 GCGGGGCTGC TAAAGCGCAT GCTCCAGACT GCCTTGGGAA AAGCGCCTCC CCTACCCGGT 5761 AGAATTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.