pCAMBIA3301-35S-LOC100281624(Z

pCAMBIA3301-35S-LOC100281624(Zea) plasmid

PVT94098

pCAMBIA3301-35S-LOC100281624(Zea) plasmid Information

Plasmid name: pCAMBIA3301-35S-LOC100281624(Zea)
Alias: NM_001154543.2;GRMZM2G318213; IDP833
Gene lenght: 369bp
Host: Plant
Use(s): Protein Expression
Fragment type: ORF
Species: corn
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling:
Promoter: 35S
Replicon: pVS1
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: NOS-R
Copy number: High
Induction:

pCAMBIA3301-35S-LOC100281624(Zea) plasmid Description

pCAMBIA3301-35S-LOC100281624(Zea) This is a plasmid suitable for protein expression in plants.

pCAMBIA3301-35S-LOC100281624(Zea) plasmid Sequence

1 catgcaggga ggtgtgagct gcgtcggcgg gcggctgggc ctgaccatcg acccggctgg 61 cgacggggag gcccccgcgg agcgcgtggg gcggttggtc cgcgagagcc ccgtggtgat 121 cttcgcctgg cgcgggtgct gcatgtgcca cgtgatgcgg cagctgctgg cggccgtggg 181 cgcgcacgcg acggtgatcg agctggagga ggccgccgag gaggccgcgg cgtcggccgc 241 cgccgccgcc gccgtgccgg cgctgttcgt gggcggcgcg cccgtgggcg ggctggacgg 301 gctcatgggg ctccacctca gcggcctcct agtcccgcgg ctccgggagg tcggcgcgct 361 ctgcggctag ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt 421 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt 481 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta 541 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa 601 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttaaactatc 661 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa 721 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa 781 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca 841 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa 901 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt 961 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc 1021 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa 1081 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc 1141 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg 1201 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag 1261 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc 1321 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc 1381 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt 1441 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag 1501 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac 1561 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc 1621 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag 1681 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga 1741 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg 1801 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca 1861 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa 1921 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca 1981 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac 2041 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga 2101 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac 2161 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga 2221 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc 2281 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga 2341 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt 2401 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc 2461 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac 2521 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt 2581 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa 2641 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct 2701 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg 2761 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag 2821 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag 2881 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc 2941 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt 3001 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca 3061 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga 3121 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt 3181 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc 3241 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct 3301 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga 3361 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg 3421 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg 3481 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc 3541 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa 3601 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc 3661 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga 3721 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc 3781 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc 3841 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc 3901 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca 3961 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc 4021 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct 4081 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag 4141 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt 4201 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc 4261 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt 4321 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat 4381 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc 4441 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg 4501 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc 4561 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc 4621 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga 4681 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag 4741 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac 4801 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 4861 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 4921 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 4981 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 5041 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 5101 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 5161 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 5221 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 5281 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 5341 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 5401 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 5461 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 5521 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 5581 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 5641 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 5701 ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta 5761 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt 5821 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc 5881 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc 5941 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa 6001 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca 6061 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat 6121 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca 6181 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat 6241 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg 6301 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg 6361 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca 6421 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga 6481 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt 6541 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact 6601 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa 6661 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc 6721 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa 6781 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc 6841 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt 6901 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt 6961 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac 7021 tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt tggttttagg 7081 aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt 7141 atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc tgggaactac 7201 tcacacatta ttatggagaa actcgagtca aatctcggtg acgggcagga ccggacgggg 7261 cggtaccggc aggctgaagt ccagctgcca gaaacccacg tcatgccagt tcccgtgctt 7321 gaagccggcc gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac 7381 gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc ttgaagccct gtgcctccag 7441 ggacttcagc aggtgggtgt agagcgtgga gcccagtccc gtccgctggt ggcgggggga 7501 gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc aggggcccgc 7561 gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg agccagggat agcgctcccg 7621 cagacggacg aggtcgtccg tccactcctg cggttcctgc ggctcggtac ggaagttgac 7681 cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt 7741 ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatg gtagactcga gagagataga 7801 tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat 7861 atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga 7921 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc 7981 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag 8041 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt 8101 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt 8161 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt 8221 agacgagagt gtcgtgctcc accatgttat cacatcaatc cacttgcttt gaagacgtgg 8281 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac 8341 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg 8401 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc 8461 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg 8521 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa 8581 gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 8641 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 8701 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 8761 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt 8821 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttggcactgg 8881 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 8941 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 9001 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg 9061 tcgtttcccg ccttcagttt agcttcatgg agtcaaagat tcaaatagag gacctaacag 9121 aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc aatgacaaga 9181 agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag 9241 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa 9301 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg 9361 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct 9421 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag 9481 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg 9541 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc 9601 atttggagag aacacggggg actcttgac

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.