pLV3-CMV-MED15-3×FLAG-CopGFP-P

pLV3-CMV-MED15-3×FLAG-CopGFP-Puro plasmid

PVT88220

pLV3-CMV-MED15-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MED15(human)-3×FLAG-CopGFP-Puro
Alias: NM_001003891.3;
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MED15-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-MED15-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MED15-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg acgtttccgg gcaagagacc 2341 gactggcgga gcaccgcctt ccggcagaag ctggtcagtc aaatcgagga tgccatgagg 2401 aaagctggtg tggcacacag taaatccagc aaggatatgg agagccatgt tttcctgaag 2461 gccaagaccc gggacgaata cctttctctc gtggccaggc tcattatcca ttttcgagac 2521 attcataaca agaaatctca agcttccgtc agtgatccta tgaatgcact ccagagcctg 2581 actggcggac ctgctgcggg agccgctgga attggcatgc ctcctcgggg cccgggacag 2641 tctctgggcg ggatgggtag ccttggtgcc atgggacagc caatgtctct ctcagggcag 2701 ccgcctcctg ggacctcggg gatggcccct cacagcatgg ctgtcgtgtc tacggcaact 2761 ccacagaccc agctgcagct ccagcaggtg gcgctgcagc agcagcagca acagcagcag 2821 ttccagcagc agcagcaggc ggcgctacag cagcagcagc agcagcagca acagcagcag 2881 ttccaggctc agcagagtgc catgcagcag cagttccaag cagtagtgca gcagcagcag 2941 cagctccagc agcagcagca gcagcagcag catctaatta aattgcatca tcaaaatcag 3001 caacagatac agcagcagca acagcagctg cagcgaatag cacagctgca gctccaacaa 3061 cagcaacagc agcagcagca gcagcagcag cagcagcagc aggctttgca ggcccagcca 3121 ccaattcagc agccaccgat gcagcagcca cagcctccgc cctcccaggc tctgccccag 3181 cagctgcagc agatgcatca cacacagcac caccagccgc caccacagcc ccagcagcct 3241 ccagttgctc agaaccaacc atcacaactc ccgccacagt cgcagaccca gcctttggtg 3301 tcacaggcgc aagctctccc tggacaaatg ttgtataccc aaccaccact gaaatttgtc 3361 cgagctccga tggtggtgca gcagccccca gtgcagcccc aggtgcagca gcagcagaca 3421 gcagtacaga cagctcaggc tgcccagatg gtggctcccg gagtccagat gatcacggaa 3481 gccttggccc aaggtgggat gcacataaga gcccggttcc cgcctaccac cgctgtgtcc 3541 gccatcccgt caagctccat ccctttgggc agacagccca tggcacaggt cagccagagc 3601 agcctcccca tgctgtcctc gccgtcaccg ggccagcagg tgcagacccc gcagtcgatg 3661 ccccctcccc cccagccgtc cccgcagccc ggccagccca gctcacagcc caactccaac 3721 gtcagctctg gccctgcccc atctcccagt agcttcctgc ccagcccctc accgcagccc 3781 tcccagagcc cagtgacggc gcggacccca cagaacttca gtgtcccctc acctggacct 3841 ttaaacacac ctgtgaaccc cagctctgtc atgagcccag ctggctccag ccaggctgag 3901 gagcagcagt acctggacaa gctgaagcag ctgtcgaagt acatcgagcc cctgcgccgc 3961 atgatcaaca agatcgacaa gaacgaagac agaaaaaagg acctgagtaa gatgaagagc 4021 cttctggaca ttctgacaga cccctcgaag cggtgtcccc tgaagacctt gcaaaagtgt 4081 gagatcgccc tggagaaact caagaatgac atggcggtgc ccactccccc accgcccccg 4141 gtgccaccga ccaaacagca gtacctatgc cagccgctcc tggatgccgt cctggccaac 4201 atccgctcac ctgtcttcaa ccattccctg taccgcacat tcgttccagc catgaccgcc 4261 attcacggcc cacccatcac ggccccagtg gtgtgcaccc ggaagcgcag gcttgaggat 4321 gatgagcggc agagcatccc cagtgtgctc cagggtgagg tggccaggct ggaccccaag 4381 ttcctggtaa acctggaccc ttctcactgc agcaacaatg gcactgtcca cctgatctgc 4441 aagctggatg acaaggacct cccaagtgtg ccaccactgg agctcagtgt gcccgctgac 4501 tatcctgccc aaagcccgct gtggatagac cggcagtggc agtacgacgc caaccccttc 4561 ctccagtcgg tgcaccgctg catgacctcc aggctgctgc agctcccgga caagcactcg 4621 gtcaccgcct tgctcaacac ctgggcccag agcgtccacc aggcctgcct ctcagccgcc 4681 ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 4741 GACGATGACA AGtagcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca 4801 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 4861 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 4921 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 4981 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 5041 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 5101 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 5161 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 5221 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 5281 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 5341 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc 5401 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag 5461 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc 5521 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac 5581 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg 5641 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc 5701 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg 5761 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc 5821 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc 5881 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag 5941 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc 6001 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 6061 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 6121 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 6181 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 6241 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 6301 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 6361 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 6421 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 6481 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 6541 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 6601 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 6661 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 6721 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 6781 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 6841 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 6901 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 6961 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 7021 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 7081 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaATC ATCGTCCTTT 7141 CCTtggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 7201 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 7261 cttccgcgtc tTcgccttcg ccctcagacg agtcggatct ccctttggGc cgcctccccg 7321 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 7381 agaaaagggg ggactggaag ggctaattca ctcccaacga aGataagatc tgctttttgc 7441 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 7501 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 7561 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 7621 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 7681 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 7741 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 7801 caaactcatc aatgtatctt atcatgtctg gctctagcta TCCCGCCCCT AACTCCGCCC 7861 Atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 7921 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 7981 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt 8041 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 8101 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 8161 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 8221 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 8281 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 8341 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 8401 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 8461 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 8521 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 8581 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 8641 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 8701 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 8761 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 8821 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 8881 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 8941 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 9001 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 9061 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 9121 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 9181 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 9241 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 9301 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 9361 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 9421 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 9481 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 9541 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 9601 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 9661 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 9721 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 9781 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 9841 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 9901 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 9961 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 10021 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 10081 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 10141 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 10201 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 10261 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 10321 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 10381 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 10441 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 10501 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 10561 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 10621 acgacgttgt aaaacgacgg ccagtgccaa gctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.