pLV2-CMV-Cnr2-Puro plasmid

pLV2-CMV-Cnr2-Puro plasmid

PVT94096

pLV2-CMV-Cnr2-Puro plasmid Information

Plasmid name: pLV2-CMV-Cnr2(rat)-Puro
Alias: NM_001164143.3;CB-2; CB2; CB2C; CNR2C; rCB2
Gene lenght: 1083bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-Cnr2-Puro plasmid Description

pLV2-CMV-Cnr2-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-Cnr2-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcgcca ccatggcggg atgccgggag ctggagttga ccaacggctc 2881 caatggcggc ttggagttca accctatgaa ggagtacatg atcttgagtg atgcgcagca 2941 gatcgctgtg gcggtgctgt gtaccctgat ggggctgctg agtgccctgg agaacgtggc 3001 tgtgctctat ctcatcctgt cctcgcagcg gctccgcagg aagccctcgt acctgttcat 3061 cggcagcttg gccggagctg acttcctggc cagcgtgatc tttgcctgca acttcgtcat 3121 cttccatgtc tttcacggtg tggactccag gaatatcttc ctgttgaaga tcggcagcgt 3181 gaccatgacc ttcacggcct ctgtgggcag cctgctgctg accgctgttg accgatacct 3241 atgtctgtgc tacccaccta cctacaaagc tctcgtcacc cgtgggaggg cactggtggc 3301 ccttggtgtc atgtgggtcc tctcggcgtt gatctcctac ctaccgctca tggggtggac 3361 ttgttgtcct agtccctgtt ctgaactttt ccccctgatc cccaacgact acctcctggg 3421 ctggcttctt ttcattgcca tcctcttttc tggcatcatc tatacctatg ggtatgtcct 3481 ctggaaagca caccaacatg tagccagctt ggctgagcac caggacaggc aggtgcctgg 3541 gatagctcgg atgcggctag acgtgaggtt ggccaagact ctgggcctgg tcatggctgt 3601 tctgctcata tgctggttcc ctgcactggc tctcatgggc catagcctgg tcaccacact 3661 gagtgacaag gtcaaggagg ccttcgcctt ctgctccatg ctgtgccttg ttaactccat 3721 gatcaatcct atcatttatg ccctgcggag tggagagatc cgctctgctg cccagcactg 3781 cctgacaggc tggaagaaat atctccaggg cctcggatct gaggggaaag aagaagcccc 3841 aaagtcctca gttacagaga cagaggctga ggtgaaaacc accacagggc cgggatccag 3901 aacaccaggc tgctccaatt gctgaggatc ccgcgactct agataattct accgggtagg 3961 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg 4021 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc 4081 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt 4141 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac 4201 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag 4261 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc 4321 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg 4381 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc 4441 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc 4501 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 4561 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 4621 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 4681 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 4741 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 4801 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 4861 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 4921 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 4981 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5041 acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga 5101 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 5161 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 5221 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 5281 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 5341 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 5401 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 5461 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 5521 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 5581 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 5641 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac 5701 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc 5761 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta 5821 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga 5881 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac 5941 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca 6001 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc 6061 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg 6121 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga 6181 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc 6241 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga 6301 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg 6361 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6421 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6481 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6541 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta 6601 gcgttttacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 6661 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 6721 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 6781 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 6841 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6961 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 7021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 7081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 7141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 7201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 7261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 7321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 7381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 7441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 7501 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 7561 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 7621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 7681 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 7741 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 7801 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 7861 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 7921 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 7981 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 8041 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 8101 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 8161 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 8221 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 8281 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 8341 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 8401 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 8461 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 8521 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 8581 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 8641 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 8701 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 8761 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac 8821 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 8881 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 8941 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca 9001 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct 9061 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt 9121 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.