pLV3-CMV-WDR4-3×FLAG-mCherry-B

pLV3-CMV-WDR4-3×FLAG-mCherry-Blast plasmid

PVT94265

pLV3-CMV-WDR4-3×FLAG-mCherry-Blast plasmid Information

Plasmid name: pLV3-CMV-WDR4(human)-3×FLAG-mCherry-Blast
Alias: NM_018669.6;GAMOS6; hWH; MIGSB; TRM82; TRMT82; Wuho
Gene lenght: 1236bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-WDR4-3×FLAG-mCherry-Blast plasmid Description

pLV3-CMV-WDR4-3×FLAG-mCherry-Blast is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-WDR4-3×FLAG-mCherry-Blast plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggcggg ctctgtggga ctggcgttgt gcgggcagac 2341 gttggtggtg cggggcggca gccgattcct ggccacctcc atagcaagca gtgatgatga 2401 cagcctcttc atctatgact gcagtgctgc agaaaagaag tcacaagaaa ataaagggga 2461 ggacgcgccc ttggaccagg ggagcggtgc gattctggcg tccaccttct ccaagtctgg 2521 cagctatttt gctttaaccg atgacagtaa gcgtctgatt cttttccgta caaaaccatg 2581 gcaatgtctg agtgtcagga ccgtggcaag gaggtgtaca gccctgactt tcatagcctc 2641 ggaggagaag gtcttggtgg ccgacaagtc tggagacgtc tactcctttt cggtgctgga 2701 gccacacggg tgtggccgtc tagagctggg gcacctgtct atgctgttag atgtggctgt 2761 gagtcctgat gaccgcttca tcctcactgc cgaccgggac gagaagatcc gagtcagctg 2821 ggccgcggcg ccccatagca tcgagtcctt ctgcttgggg cacacagagt ttgtgagccg 2881 tatctccgtg gtgccaactc agcccgggct gcttctgtcc tcctctgggg acggcaccct 2941 gaggctctgg gagtacagga gcggccgcca gctgcactgc tgtcacctgg ccagtctgca 3001 ggagctggtg gacccccagg ccccccagaa gtttgccgcg tccaggattg cattctggtg 3061 ccaggagaac tgcgtggcgc tcctgtgcga cggcactcct gtggtctaca tcttccagct 3121 ggacgcccgc agacagcagt tggtgtacag gcagcagctg gcgttccagc accaagtgtg 3181 ggacgtggct ttcgaggaga cccaggggct gtgggtgctc caggactgcc aggaagcccc 3241 cctggtgctc tacaggcctg tgggcgacca gtggcagtct gttcctgaga gcaccgtgtt 3301 aaagaaagtc tctggtgttc ttcgtgggaa ctgggccatg ctggaaggct ctgccggcgc 3361 agacgccagc ttcagcagtc tctacaaggc cacgttcgac aacgtgacct cctacctgaa 3421 gaagaaagag gagagactgc agcagcagct agagaagaag cagcggcgcc ggagtccccc 3481 gcctgggccc gacgggcatg ccaagaagat gagaccgggg gaggcgacgc taagttgcgg 3541 atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA 3601 CGATGACAAG tagggatcct gtatggcggc cgcGaaggat ctgcgatcgc tccggtgccc 3661 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca 3721 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact 3781 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga 3841 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca 3901 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg 3961 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa 4021 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc 4081 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg 4141 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggtga 4201 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca 4261 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct 4321 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct 4381 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg 4441 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga 4501 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt 4561 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga 4621 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga 4681 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca 4741 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca 4801 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg 4861 agggccgcca ctccaccggc ggcatggacg agctgtacaa gggatctcgc gagggcagag 4921 gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctATGGCC AAGCCTTTGT 4981 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT 5041 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG 5101 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG 5161 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA 5221 TCTTGAGCCC CTGCGGACGG TGCCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG 5281 CCATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT 5341 CTGGTTATGT GTGGGAGGGC TAAGTCGACa atcaacctct ggattacaaa atttgtgaaa 5401 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 5461 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 5521 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 5581 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 5641 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 5701 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 5761 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 5821 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 5881 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc 5941 tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc 6001 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat 6061 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 6121 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 6181 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 6241 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 6301 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg 6361 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 6421 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc 6481 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 6541 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 6601 ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt 6661 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 6721 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 6781 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 6841 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 6901 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 6961 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 7021 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7081 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7141 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 7201 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 7261 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7321 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7381 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7441 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 7501 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7561 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7621 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7681 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 7741 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 7801 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 7861 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 7921 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 7981 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 8041 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 8101 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 8161 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 8221 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 8281 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 8341 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 8401 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 8461 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 8521 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 8581 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 8641 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 8701 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 8761 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 8821 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 8881 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 8941 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 9001 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 9061 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 9121 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 9181 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 9241 acgacgttgt aaaacgacgg ccagtgccaa gctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.