pLV3-CMV-Lima1-3×FLAG-CopGFP-P

pLV3-CMV-Lima1-3×FLAG-CopGFP-Puro plasmid

PVT94067

pLV3-CMV-Lima1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Lima1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_001113545.1;Eplin
Gene lenght: 2262bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Lima1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Lima1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Lima1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccATGG AATCGACTCC ATTTAATAGA 2341 CGCCAGTGGA CTTCCCTGTC ATTGAGAGTA ACAGCCAAAG AGCTTTCTCT TGTCAACAAG 2401 AACAAGTCAT CCGCAATTGT GGAAATATTC TCCAAGTACC AGAAGGCTGC TGAAGAAGCC 2461 AACATGGAAA GGAAGAAAAA TAACCCTGAA AGTCTGCCCC AGCACTTTAG AAGAGGGACC 2521 CTGTCTGTGT TAAAGAAGAA GTGGGAGAAC CCGGTGGCTG GGGCAGAATT CCACACAGAC 2581 TCACTGCCAA ACAGCAGCAG TGAGGGTGGG CACACAGCGG ACTACCCTCC TGCTGAAGTG 2641 ACGGACAAGC CTGCTCCTGG AGTCAGAGCT GACCGGGAAG AGCACACCCA GCCCAAACCT 2701 AGATTTGGAT CTCGTCCGGA AGCAGTTATC CAGAGCCGGT ATCCTCGCTC AGAGAACAGC 2761 CACGATTTTA AAGCCCAGGC CACGGAGAGC CAAAAAATGG AAAACTGTCT GGGAGATTCC 2821 AGGCATGAAG CAGAGAAGCC AGAGACGAGC GAAAACACAG AAACTTCAGG CAAAATAGAG 2881 AAATACAACG TTCCACTGAA TAGACTGAAG ATGATGTTTG AGAAAGGTGA ACACAACCAA 2941 ACCAAGAGTC TCTGGACCCA AAGCCGAAAT GCGGGTGGAA GGAGGCTCTC TGAAAACAAC 3001 TGTTCCCTGG ATGACTGGGA AATAGGTGCG GGTCATTTGT CTTCCTCTGC ATTCAACTCG 3061 GAGAAAAACG AGAGTAAGAG GAATCTGGAG CTGCCACGCC TCTCAGAAAC CTCCATAAAG 3121 GACCGCATGG CCAAGTACCA GGCTGCAGTG TCCAAGCAGA GCAGCCCAGC CAGCTACACA 3181 AATGAGCTGA AAACCAGTGA AAGCAAAACT CATAAATGGG AACAGAAGGA GAATGTGCCC 3241 CCAGGTCCCG AGGCCTGCAG CGTCCATCAG GAAGGAAGCA AGGTTTCTAC AACTGAGAAT 3301 AGTCTAGTGG CCCTCTCTGT CCCTGCTGAA GATGACACCT GTAACTCCCA GGTGAAGAGC 3361 GAGGCCCAGC AGCCTATGCA CCCTAAGCCG CTGAGTCCTG ACGCCAGGAC CTCCAGTCTT 3421 CCGGAAAGTT CTCCTTCCAA AACAGCGAAG AAGTTTCAGG CGCCGGCAAA GGAGAGCTGC 3481 GTGGAGTGTC AGAAGACGGT GTACCCCATG GAACGGCTCC TGGCCAACCA GCAGGTGTTT 3541 CACATCAGCT GTTTCCGATG CTCCTACTGC AACAACAAGC TCAGTCTAGG AACATATGCA 3601 TCCTTACATG GACGAATCTA CTGTAAGCCT CACTTCAATC AACTCTTCAA GTCCAAAGGC 3661 AACTATGACG AGGGCTTTGG GCACAAGCAG CATAAGGATC TGTGGGCAAG CAAGAGCGAC 3721 AATGAGGAGA CTTTGGGGAG ACCAGCCCAG CCGCCTAATG CAGGGGAGAG CCCCCATAGC 3781 CCGGGTGTAG AAGATGCCCC CATCGCCAAG GTCGGCGTGC TGGCGGCAAG TATGGAAGCC 3841 AAGGCCTCTT CTCAGAGGGA GAGGGAGGAT AAGCCTGCGG AGACCAAGAA GCTGAGGATC 3901 GCCTGGCCTC CGCCAGCCGA GCTGGGCGGT TCCGGAAGTG CCCTGGAGGA AGGGATCAAA 3961 GTATCGAAGC CCAAGTGGCC TCCGGAGGAT GACGTCTGCA AGACGGAGGC CCCGGAGGAT 4021 GTAGATCTGG ACCTGAAGAA GCTGCGGCGG TCTTCGTCCC TGAAGGAGAG GAGCCGCCCG 4081 TTTACGGTAG CAGCTTCCTT TCGAACCTCT TCCATCAAGA GCCCCAAGGC CTCTTCGCCA 4141 TCTCTCAGGA AAGGTTGGAG CGAGTCCGAG CAGAGTGAGG AGTTTGGAGG AGGGATAGCG 4201 ACGATGGAAA GGAAACAAAC GGAAAATGCC AGACCCTCTG GGGAGAAAGA GAATGTGGGA 4261 AAATCACGCT GGCAGGGCGA GGAAGTGCCG AGGAGCAAGG ACCGTAGTTC TTTTGAGCTG 4321 GAGAGTGAGA ATTTTATGGA AAATGGAGCA AACATAGCTG AAGATGACAA CCATGTCCAC 4381 GCACAGCAGT CCCCGTTAGA ACCCGAGGCT CCGGGTTGGT CTGGCTTTGT AGACACCACG 4441 GCTGCTAAAG AATTCACTAC CCAGAATCAA AAATCCCAAG ATGTCGGGTT CTGGGAGGGA 4501 GAAGTGGTCC GAGAGCTGTC TGTGGAGGAA CAGATAAAGA GAAACCGGTA CTATGATGAG 4561 GACGAGGATG AAGAAggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 4621 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 4681 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 4741 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 4801 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 4861 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 4921 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 4981 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 5041 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 5101 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 5161 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 5221 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 5281 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 5341 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 5401 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 5461 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 5521 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 5581 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 5641 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 5701 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 5761 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 5821 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 5881 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 5941 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 6001 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 6061 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 6121 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 6181 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 6241 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 6301 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 6361 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 6421 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 6481 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 6541 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 6601 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 6661 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 6721 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 6781 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 6841 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 6901 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 6961 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 7021 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 7081 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 7141 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 7201 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 7261 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 7321 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 7381 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 7441 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 7501 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 7561 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 7621 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 7681 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 7741 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 7801 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 7861 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 7921 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 7981 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 8041 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 8101 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 8161 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 8221 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 8281 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 8341 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 8401 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 8461 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 8521 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 8581 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 8641 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 8701 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 8761 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 8821 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 8881 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 8941 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 9001 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 9061 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 9121 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 9181 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 9241 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 9301 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 9361 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 9421 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 9481 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9541 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9601 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9661 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 9721 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9781 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 9841 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9901 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9961 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 10021 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 10081 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 10141 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 10201 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 10261 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 10321 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 10381 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 10441 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 10501 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.