pCMV-SREBF2-3×HA-Puro plasmid

pCMV-SREBF2-3×HA-Puro plasmid

PVT89327

pCMV-SREBF2-3×HA-Puro plasmid Information

Plasmid name: pCMV-SREBF2(human)-3×HA-Puro
Alias: NM_004599.4;bHLHd2; SREBP-2; SREBP2;
Gene lenght: 3423bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature:
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SREBF2-3×HA-Puro plasmid Description

pCMV-SREBF2-3×HA-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-SREBF2-3×HA-Puro plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCGCCACC atggacgaca gcggcgagct gggtggtctg 961 gagaccatgg agaccctcac ggagctgggc gacgagctga ccctgggaga catcgacgag 1021 atgctgcaat ttgtcagtaa tcaagtggga gagttccctg acttgttttc agaacagctg 1081 tgtagctcct ttcctggcag tggtggtagt ggtagcagca gcggcagcag tggcagcagc 1141 agcagcagca gcaatggcag gggcagcagc agcggagctg tggacccttc agtgcaacgg 1201 tcattcaccc aggtcacatt accttccttc tctccctcgg cggcctcccc acaggctcca 1261 actctgcaag tcaaggtttc tcccacctca gttcccacca cacccagggc aactcctatt 1321 cttcagcccc gcccccagcc ccagcctcaa cctcaaactc agctgcaaca acagacggta 1381 atgatcacgc caacattcag caccactccg cagacgagga tcatccagca gcctttgata 1441 taccagaatg cagctactag ctttcaagtc cttcagcctc aagtccaaag cctggtgaca 1501 tcctcccagg tacagccggt caccattcag cagcaggtgc agacagtaca ggcccagcgg 1561 gtgctgacac aaacggccaa tggcacgctg cagacccttg ccccggctac ggtgcagaca 1621 gttgctgcgc cacaggtgca gcaggtcccg gtcctggtcc agcctcagat catcaagaca 1681 gattcccttg ttttgaccac actgaagaca gatggcagcc ctgttatggc tgcggtccag 1741 aacccggccc tcaccgccct caccacccct atccagacgg ctgcccttca agtaccaacc 1801 ctggtgggca gcagtgggac cattctgacc acaatgcctg taatgatggg gcaagagaaa 1861 gtgcccatta agcaggtacc tgggggagtc aagcagcttg agccccccaa agaaggagaa 1921 aggcggacaa cccataatat cattgagaaa cgatatcgct cctccatcaa tgacaaaatc 1981 atcgaattga aagacctggt catggggaca gacgccaaga tgcacaagtc tggcgttctg 2041 aggaaggcca ttgattacat caaatacttg cagcaggtca atcataaact gcgccaggag 2101 aacatggtgc tgaagctggc aaatcaaaag aacaagcttc taaagggcat cgacctaggc 2161 agtctggtgg acaatgaggt ggacctgaag atcgaggact ttaatcagaa tgtccttctg 2221 atgtcccccc cagcctctga ctcagggtcc caggctggct tctctcccta ctccattgac 2281 tctgagccag gaagccctct attggatgat gcaaaggtca aagatgagcc agactctcct 2341 cctgtggcgc tgggcatggt agaccgctca cggattcttc tgtgtgtcct caccttcctg 2401 tgcctctcct ttaaccccct gacttccctg ctgcagtggg gaggggccca cgactctgac 2461 cagcacccac actcaggctc tggccgcagt gtcctgtcat tcgagtcagg ttctgggggc 2521 tggtttgact ggatgatgcc tactcttctc ttatggctgg taaatggtgt gattgtcctg 2581 agcgtctttg tgaagctgct ggttcatggg gagccagtga tccggccaca ctcgcgctcc 2641 tcggtcacct tctggaggca ccggaaacag gcagatctgg atctcgccag aggagatttt 2701 gcagctgctg ccggcaacct acaaacctgc ctggcagttt tgggccgggc actgcccacc 2761 tcccgcctgg acctggcctg cagcctctcc tggaacgtga tccgctacag cctgcagaag 2821 ctacgcctgg tgcgctggct gctcaagaaa gtcttccagt gccggcgggc cacgccagcc 2881 actgaggcag gctttgaaga cgaagctaag accagcgccc gggatgcggc tctggcctat 2941 caccggctgc accagctgca catcacaggg aagcttcctg caggatccgc ctgttccgat 3001 gtacacatgg cgttgtgtgc cgtgaacctg gctgaatgtg cagaggagaa gatcccaccg 3061 agcacactgg ttgagatcca tctgactgct gccatggggc tcaagacccg gtgtggaggc 3121 aagctgggct tcctggccag ctacttcctc agccgagccc agagcctgtg tggccccgag 3181 cacagtgctg ttcctgactc cctgcgctgg ctctgccacc ccctgggcca gaagtttttc 3241 atggagcgga gctggtctgt gaagtcagct gccaaggaga gtctatactg tgcccagagg 3301 aacccagctg accccattgc gcaggtccac caggccttct gcaagaacct gctggagcga 3361 gctatagagt ccttggtgaa acctcaggcc aagaagaagg ctggagacca ggaagaagag 3421 agctgtgaat tctccagtgc tctggagtac ttgaaattac ttcattcttt tgtggactct 3481 gtgggggtta tgagcccccc actctccagg agctccgtgc tcaagtccgc cctgggtcca 3541 gacatcatct gtcggtggtg gacgtctgca atcactgtgg ccatcagctg gctccaggga 3601 gacgatgcag ctgtgcgctc tcattttacc aaagtggaac gcatccccaa ggccctggaa 3661 gtgacagaga gccccctggt gaaggccatc ttccatgcct gcagagccat gcatgcctca 3721 ctccctggga aagcagatgg gcagcagagt tccttctgcc attgcgagag ggccagtggc 3781 cacctatgga gcagcctcaa cgtcagtggg gccacctctg accctgccct caaccacgtg 3841 gtccagctgc tcacctgtga cctgctactg tcgctacgga cagcgctctg gcaaaaacag 3901 gccagtgcca gccaggctgt gggggagacc taccacgcgt caggcgctga actggcgggc 3961 ttccaacggg acctgggcag cctgcgcagg ctggcacaca gcttccgccc agcataccgc 4021 aaggtgttcc tgcatgaagc caccgtgcgc ctgatggcag gagccagccc cacccgcacc 4081 caccagctgc tggaacacag cctgcggcgg cgcaccacgc agagcaccaa gcacggagag 4141 gtggatgcct ggcccggcca gcgagagcgg gccaccgcca tcctgctggc ctgccgccac 4201 ctgcccctct ccttcctctc ctccccgggc cagcgggcag tgctgctggc cgaagctgcc 4261 cgcaccctgg agaaggtggg cgaccggcgc tcctgcaacg actgccagca gatgattgtt 4321 aagctgggtg gtggcactgc cattgccgcc tccCTCGAGT ACCCATACGA CGTACCTGAT 4381 TATGCAACTT ATCCTTATGA TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC 4441 TACGCAtagT CTAGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT 4501 TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT 4561 CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT 4621 TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC 4681 AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC 4741 TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 4801 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 4861 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 4921 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 4981 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 5041 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 5101 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 5161 ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA 5221 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 5281 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 5341 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA 5401 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG 5461 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT 5521 TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT 5581 GAGGATCGTT TCGCatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg 5641 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca 5701 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc 5761 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct 5821 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg 5881 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc 5941 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg 6001 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg 6061 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct 6121 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca 6181 agcccggtgc ctgaGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT 6241 GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT 6301 TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC 6361 CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA 6421 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA 6481 TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC 6541 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG 6601 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT 6661 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG 6721 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA 6781 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 6841 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 6901 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 6961 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 7021 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 7081 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 7141 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 7201 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 7261 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 7321 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 7381 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 7441 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT 7501 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 7561 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 7621 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 7681 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 7741 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 7801 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 7861 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 7921 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 7981 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 8041 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 8101 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 8161 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 8221 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 8281 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 8341 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 8401 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 8461 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 8521 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG 8581 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 8641 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC 8701 GGGAGATCTC CCGATCCCCT ATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.