pDC316-CMV-mCherry-U6-FZR1-shR

pDC316-CMV-mCherry-U6-FZR1-shRNA2 plasmid

PVT93662

pDC316-CMV-mCherry-U6-FZR1-shRNA2 plasmid Information

Plasmid name: pDC316-CMV-mCherry-U6-FZR1(human)-shRNA2
Alias: AGAAGGGTCTGTTCACGTATT
Gene lenght:
Host: Mammalian cells,Adenovirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pDC316-CMV-mCherry-U6-FZR1-shRNA2 plasmid Description

pDC316-CMV-mCherry-U6-FZR1-shRNA2 is a plasmid suitable for use in mammalian cells, and can also be used for adenovirus packaging.

pDC316-CMV-mCherry-U6-FZR1-shRNA2 plasmid Sequence

1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg 481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc 541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa 601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa 661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc 721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat 781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg 841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct 901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca 961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcca ccatggtgag caagggcgag 1021 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc 1081 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc 1141 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg 1201 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac 1261 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 1321 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag 1381 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg 1441 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc 1501 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac 1561 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc 1621 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac 1681 tccaccggcg gcatggacga gctgtacaag tagagatctc gagatccacc ggatctagat 1741 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc 1801 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta 1861 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 1921 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtct 1981 gggcaggaag agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct 2041 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg 2101 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg 2161 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttgggttt atatatcttg 2221 tggaaaggac gaaacacctg cagttttttg gatccgAGAA GGGTCTGTTC ACGTATTctc 2281 gagAATACGT GAACAGACCC TTCTTTTTTT gtcgacttcg agcaacttgt ttattgcagc 2341 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 2401 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcgt 2461 ctagcatcga agatccaata acttcgtata gcatacatta tacgaagtta taagtagctt 2521 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 2581 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 2641 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 2701 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 2761 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 2821 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 2881 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 2941 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3001 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 3061 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 3121 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 3181 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 3241 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 3301 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 3361 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 3421 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 3481 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 3541 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 3601 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 3661 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 3721 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 3781 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 3841 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 3901 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 3961 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 4021 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 4081 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 4141 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 4201 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 4261 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa 4321 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 4381 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 4441 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 4501 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 4561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 4621 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 4681 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 4741 tctaggcaaa atagcaccct cccgctccag aacaacatac agcgcttcac agcggcagcc 4801 taacagtcag ccttaccagt aaaaaagaaa acctattaaa aaaacaccac tcgacacggc 4861 accagctcaa tcagtcacag tgtaaaaaag ggccaagtgc agagcgagta tatataggac 4921 taaaaaatga cgtaacggtt aaagtccaca aaaaacaccc agaaaaccgc acgcgaacct 4981 acgcccagaa acgaaagcca aaaaacccac aacttcctca aatcgtcact tccgttttcc 5041 cacgttacgt aacttcccat tttaagaaaa ctacaattcc caacacatac aagttactcc 5101 gccctaaaac ctacgtcacc cgccccgttc ccacgccccg cgccacgtca caaactccac 5161 cccctcatta tcatattggc ttcaatccaa aataaggtat a

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.