pLV3-CMV-Sgk1-3×FLAG-CopGFP-Pu

pLV3-CMV-Sgk1-3×FLAG-CopGFP-Puro plasmid

PVT93431

pLV3-CMV-Sgk1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Sgk1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_001161845.2;Sgk
Gene lenght: 1572bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Sgk1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Sgk1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Sgk1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagaGCCACC atggtaaaca aagacatgaa tggattcccg gtcaagaaat gctcagcgtt 2341 ccaatttttt aagaaacggg tacgaagatg gatcaagagc cccatggtca gcgtggacaa 2401 gcatcagagc cccaacttga agtacactgg ccctgctggg gtgcatcttc cccctgggga 2461 gtcagacttt gaggccatgt gtcaatcatg cctgggtgac catgctttcc aaagggggat 2521 gctccctcca gaggagtcct gttcctggga gatccaacct gggtgtgaag tgaaagaaca 2581 atgtaatcat gccaacatcc tgaccaagcc ggacccaaga accttctgga ctaatgatga 2641 tgcagctttt atgaaacaga gaaggatggg cctgaacgat tttattcaga agattgccag 2701 caacacctat gcatgcaaac acgctgaagt tcagtccatt ttgaaaatgt cccatcctca 2761 ggagccggag cttatgaacg ctaacccctc tcctccgcca agtccctctc aacaaatcaa 2821 cctgggtccg tcctccaacc ctcacgccaa accctccgac tttcacttct tgaaagtgat 2881 cggaaagggc agttttggaa aggttcttct ggctaggcac aaggcagaag aagtattcta 2941 tgcagtcaaa gttttacaga agaaagccat cctgaagaag aaagaggaga agcatattat 3001 gtcagagcgg aatgttctgt tgaagaatgt gaagcaccct ttcctggtgg gccttcactt 3061 ctcattccag accgctgaca agctctactt tgtcctggac tacattaatg gtggagagct 3121 gttctaccat ctccagaggg agcgctgctt cctggaacca cgggctcgat tctacgcagc 3181 tgaaatagcc agtgccctgg gctatctgca ctccctaaac atcgtttata gagacttaaa 3241 acctgagaat attctcctag actcccaggg gcacatcgtc ctcactgact ttgggctctg 3301 caaagagaat attgagcata acgggacaac atctaccttc tgtggcacgc ctgagtatct 3361 ggctcctgag gtcctccata agcagccgta tgaccggacg gtggactggt ggtgtcttgg 3421 ggctgtcctg tatgagatgc tctacggcct gcccccgttt tatagccgga acacggctga 3481 gatgtacgac aatattctga acaagcctct ccagttgaaa ccaaatatta caaactcggc 3541 aaggcacctc ctggaaggcc tcctgcagaa ggaccggacc aagaggctgg gtgccaagga 3601 tgactttatg gagattaaga gtcatatttt cttctcttta attaactggg atgatctcat 3661 caataagaag attacacccc catttaaccc aaatgtgagt gggcccagtg accttcggca 3721 cttcgatccc gagtttaccg aggagccggt ccccagctcc atcggcaggt cccctgacag 3781 catccttgtc acggccagtg tgaaggaagc agcagaagcc ttcctcggct tctcctatgc 3841 acctcctgtg gattccttcc tcgaattcat cggatccGAC TACAAAGACC ATGACGGTGA 3901 TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcGaaggat 3961 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa 4021 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg 4081 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat 4141 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct 4201 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca 4261 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc 4321 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg 4381 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta 4441 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac 4501 gctagacgcc accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg 4561 catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc 4621 caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc 4681 ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg 4741 ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga 4801 gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg 4861 cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac 4921 cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt 4981 gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt 5041 ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg 5101 ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt 5161 ggagtaccag cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc 5221 caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg 5281 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc 5341 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 5401 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 5461 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 5521 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 5581 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 5641 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 5701 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 5761 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 5821 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 5881 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta 5941 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 6001 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 6061 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 6121 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 6181 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 6241 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 6301 cgtggtgttg tcggggaaAT CATCGTCCTT TCCTtggctg ctcgcctgtg ttgccacctg 6361 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 6421 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt ctTcgccttc gccctcagac 6481 gagtcggatc tccctttggG ccgcctcccc gcctggtacc tttaagacca atgacttaca 6541 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc 6601 actcccaacg aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 6661 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 6721 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 6781 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 6841 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 6901 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 6961 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 7021 ggctctagct aTCCCGCCCC TAACTCCGCC CAtcccgccc ctaactccgc ccagttccgc 7081 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 7141 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag 7201 agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 7261 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 7321 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 7381 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 7441 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 7501 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 7561 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 7621 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7681 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7741 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7801 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7861 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7921 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 7981 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 8041 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 8101 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 8161 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 8221 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 8281 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 8341 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 8401 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 8461 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 8521 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 8581 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 8641 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 8701 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 8761 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 8821 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 8881 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 8941 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 9001 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 9061 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 9121 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 9181 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 9241 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 9301 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 9361 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 9421 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 9481 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 9541 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 9601 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 9661 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 9721 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 9781 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 9841 agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.