pCMV-3×Myc-WDR6-Neo plasmid

pCMV-3×Myc-WDR6-Neo plasmid

PVT91577

pCMV-3×Myc-WDR6-Neo plasmid Information

Plasmid name: pCMV-3×Myc-WDR6(human)-Neo
Alias: NM_018031.6;Trm734;
Gene lenght: 3366bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pCMV-3×Myc-WDR6-Neo plasmid Description

pCMV-3×Myc-WDR6-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×Myc-WDR6-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CTCTAGAATG AGCGGCCGCT CTGAGCAAAA GCTCATTTCT GAAGAGGACT TGAATGGAGA 961 ACAGAAATTG ATCAGTGAGG AAGACCTCAA CGGTGAGCAG AAGTTAATAT CCGAGGAGGA 1021 TCTTAATAGT AGCGGCCGCG GTatggacgc tctcgaggac tacgtttggc cgcgggcaac 1081 ctcggagctt atactcctcc cagtgacggg tctggagtgc gtgggggacc ggctgttggc 1141 gggtgagggt cccgatgtcc tggtgtacag cttggacttt ggtgggcatc tgcggatgat 1201 aaagcgagtg cagaacctgc ttggccacta tcttatccat ggcttccggg tacggccaga 1261 gcctaatgga gaccttgact tggaggccat ggtggctgtg tttggaagca agggactccg 1321 agttgtgaaa attagctggg gacagggcca cttctgggag ctttggcgct ctggcctgtg 1381 gaacatgtct gactggattt gggatgcacg ctggcttgag ggaaatatag ccttggccct 1441 gggccacaac tcagtggtgc tatatgaccc tgtagtaggg tgcatcctgc aagaggtgcc 1501 ctgcacagac aggtgcaccc tctcttcagc ctgcctgatt ggagacgcct ggaaggagct 1561 gaccatagtg gcaggtgctg tttccaacca gctcttggtc tggtacccag caactgcctt 1621 agcagacaac aaacctgtag cacctgaccg acgaatcagt gggcatgtgg gcatcatctt 1681 cagcatgtca tacctggaaa gcaagggatt gctggctaca gcttcagaag accgaagcgt 1741 tcgtatctgg aaggtgggcg acctgcgagt gcctgggggt cgggtgcaga atattgggca 1801 ctgctttggg cacagcgccc gtgtgtggca ggtcaagctt ctagagaatt accttatcag 1861 tgcaggagag gattgtgtct gcttggtgtg gagccatgaa ggtgagatcc tccaggcctt 1921 tcggggacac cagggacgtg ggatccgggc catagctgcc catgagaggc aggcctgggt 1981 gatcactggg ggtgatgact caggcattcg gctgtggcac ttggtagggc gtgggtaccg 2041 gggattgggg gtctcggctc tctgcttcaa gtcccgtagt aggccaggta cactcaaggc 2101 tgtgactctg gctggctctt ggcgactgct ggcagtgact gatacagggg ccctgtatct 2161 ctatgacgtc gaggtcaagt gctgggagca gctgctagag gataaacatt tccagtccta 2221 ctgcctgctg gaggcagctc ctggtcccga gggcttcgga ttgtgtgcta tggccaatgg 2281 ggaaggtcgt gtcaaggttg tccccatcaa cactccaact gctgctgtgg accagaccct 2341 gtttcctggg aaggtgcaca gcttgagctg ggccctgcgt ggttatgagg agctcctgtt 2401 gctggcatcg ggccctggcg gggtagtagc ttgcctagag atctcagccg caccctctgg 2461 caaggccatc tttgtcaagg aacgttgtcg gtacctgctg cccccaagca agcagagatg 2521 gcacacatgc agtgccttcc tacccccagg tgacttcctg gtgtgtggtg accgccgggg 2581 ctctgtgctg ctattcccct ccagaccagg tctgctcaag gaccctgggg tgggaggcaa 2641 ggctcgggct ggtgctgggg cacctgtagt gggtagtggt agtagtgggg gtgggaatgc 2701 tttcactggg ttgggcccag tgtctaccct gccctctctg cacgggaagc agggtgtgac 2761 ctcagtcaca tgccatggtg gctatgtgta taccacaggg cgtgatggag cctactacca 2821 gctgtttgta cgagacggcc agctccagcc agtcctaagg cagaagtcct gtcgaggcat 2881 gaactggcta gctgggctcc gtatagtgcc cgatgggagc atggttatcc tgggtttcca 2941 tgccaatgag tttgtggtgt ggaaccctcg gtcacacgag aagctgcaca tcgtcaactg 3001 tggtggaggg caccgttcgt gggcattctc tgatactgag gcggccatgg cctttgctta 3061 cctcaaggat ggggatgtca tgctgtacag ggctctgggt ggctgcaccc ggccacacgt 3121 gattctccgg gagggtctgc atggccgtga gatcacttgt gtaaagcgtg tgggcaccat 3181 taccctgggg cctgaatatg gagtgcccag cttcatgcag cctgatgacc tggagcctgg 3241 cagtgagggg cccgacttga ctgacattgt gatcacatgt agtgaggaca ctactgtctg 3301 tgtcctagca ctccctacaa ccacaggctc agcccacgca ctcacagctg tttgtaacca 3361 tatctcctcg gtacgtgctg tggctgtgtg gggcattggc accccaggtg gccctcagga 3421 tcctcagcca ggcctgactg cccatgtggt gtctgcgggg gggcgggctg agatgcactg 3481 cttcagcatc atggttactc cggaccccag caccccaagc cgcctcgcct gccatgtcat 3541 gcacctttcg tcccaccggc tagatgagta ttgggaccgg caacgcaatc ggcatcggat 3601 ggttaaggta gacccagaga ccaggtacat gtcccttgct gtgtgtgaac ttgaccagcc 3661 cggccttggc ccccttgtgg ctgcagcctg tagtgatggg gccgtaaggc tctttctttt 3721 gcaggattct gggcggattc tgcagctcct tgctgaaacc ttccaccata agcgatgtgt 3781 cctcaaggtc cactccttta cacacgaggc acccaaccag aggcggaggc tcctcctgtg 3841 cagcgcagct actgatggca gcctggcttt ctgggatctc accaccatgc tagaccatga 3901 ctccactgtc ctggagcctc cagtggatcc tgggcttccc taccggcttg gcaccccctc 3961 cctgactctc caggcccaca gctgtggtat caacagcctg cacaccttgc ccacccgtga 4021 gggccaccat ctcgtggcca gtggcagtga agatggatcc ctccatgtct tcgtgcttgc 4081 tgtggagatg ctacagctag aagaggctgt gggagaggct gggctggtac cccagctgcg 4141 tgtgctagag gaatactctg tcccctgtgc acatgctgcc catgtgacag gcctcaagat 4201 cctaagccca agcatcatgg tctcagcctc cattgatcaa cggctgacct tctggcgtct 4261 ggggcatggt gaacccacct tcatgaatag cactgtgttc catgtgcctg atgtggctga 4321 catggactgc tggcctgtga gccctgagtt tggccaccgt tgtgcccttg ggggtcaggg 4381 gcttgaggtt tacaactggt atgactgaGA ATTCCTGCAG ATATCCAGCA CAGTGGCGGC 4441 CGCTCGAGTC TAGAGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT 4501 GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC 4561 CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT 4621 CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA 4681 GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT 4741 CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 4801 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 4861 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 4921 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 4981 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 5041 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 5101 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 5161 TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA 5221 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC 5281 CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 5341 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG 5401 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC 5461 CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT 5521 TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG 5581 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT 5641 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT 5701 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC 5761 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC 5821 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA 5881 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT 5941 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA 6001 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA 6061 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC 6121 GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT 6181 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA 6241 CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG 6301 GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT 6361 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA 6421 GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG 6481 GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG 6541 CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC 6601 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG 6661 TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG 6721 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC 6781 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC 6841 ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG 6901 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT 6961 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC 7021 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 7081 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 7141 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 7201 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 7261 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 7321 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 7381 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 7441 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 7501 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 7561 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 7621 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT 7681 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT 7741 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 7801 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 7861 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 7921 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 7981 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC 8041 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 8101 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 8161 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 8221 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 8281 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 8341 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 8401 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 8461 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 8521 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 8581 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 8641 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 8701 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 8761 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 8821 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 8881 GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.