pLV3-CMV-Padi2-3×FLAG-CopGFP-P

pLV3-CMV-Padi2-3×FLAG-CopGFP-Puro plasmid

PVT93406

pLV3-CMV-Padi2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Padi2(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_008812.3;mKIAA0994; Pdi; Pdi2
Gene lenght: 2022bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Padi2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Padi2-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Padi2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgc agccgcctat acgggaaaat 2341 atgctgcggg aacggaccgt gcggctgcag tatgggagcc gcgtggaggc ggtgtacgtg 2401 ctgggcacgc agctctggac cgatgtctac agtgcagcac ctgctggggc caaaaccttc 2461 agcctgaagc actcggaagg tgtgaaggtg gaggtcgtac gtgatggaga ggcagaggag 2521 gtggtcacca acggcaagca gcgctgggcc ctgtcaccca gcagcacact gcggctcagc 2581 atggcccagg ccagcacaga ggccagcagc gacaaggtca ctgtcaacta ctatgaagag 2641 gagggcagcg cccccattga ccaggctggg ctcttcctca cggccattga gatctcgctg 2701 gatgtggacg cagatcggga tggagaggtg gagaagaaca atcctaagaa ggcatcctgg 2761 acctggggtc ctgagggcca aggggctatc ctgctggtga attgtgaccg agacacaccc 2821 tggttgccca aggaggactg tagtgacgaa aaggtctaca gcaagcaaga cctccaggac 2881 atgtctcaga tgatcctgcg caccaaaggc cctgaccgcc tgcccgccgg gtatgaaata 2941 gtcctctaca tctccatgtc agactcggac aaagtgggcg tcttctacgt ggagaacccg 3001 ttctttggcc agcgctatat ccacatcctg ggccggcaga agctctacca cgtggtcaag 3061 tacaccggcg gttctgcgga gttgctgttc tttgtggaag gcctctgttt ccccgacgag 3121 agtttctcag gcctggtttc tatccacgtc agtctgctgg agtatatggc cgagggcatc 3181 ccgttgaccc ccatcttcac agacactgtg atgttccgga ttgcaccctg gattatgacc 3241 cccaacatct tgcctcctgt gtcagtgttc gtgtgctgca tgaaggataa ttacctgttc 3301 ctgaaagagg tgaagaactt ggtggagaaa accaactgcg aactgaaagt ttgtttccag 3361 tacatgaacc gcggtgaccg ctggatccag gatgaaatcg agtttggcta cattgaggca 3421 ccccacaaag gcttcccagt agtgctggac tcccctcgag atggaaacct gaaggacttc 3481 cccattaagc aactcctggg cccagatttc ggctatgtga cccgggagcc cctctttgag 3541 actgtcacca gcctcgactc cttcgggaat ctggaggtca gtcccccagt gaccgtgaat 3601 ggcaaggagt acccgcttgg ccggatcctc atcggaagca gtttccccct gtctggaggc 3661 aggaggatga ccaaggtggt acgcgacttc ctgcaggccc agcaggtgca ggcgcccgtg 3721 gaactctact ccgactggct gactgtaggc cacgtcgatg agtttatgac tttcatcccc 3781 atcccaggca aaaaggaatt tcggctgctc atggccagca cctccgcctg ctaccagctc 3841 ttccgagaaa agcagaaggc aggccacggg gaggccgtta tgttcaaggg cctgggaggc 3901 atgagcagca agcgcatcac catcaacaag atcctgtcca atgagagcct cacgcaggag 3961 aaccagtact tccagcgctg tctggactgg aaccgcgaca tccttaagag ggagctagcg 4021 ctgactgaga aggacattat tgacctgccc gcgctcttca agatggatga aaatcaccag 4081 gccagggctt tcttccctaa catggtgaac atgatcgtgc tagacaagga cctgggcatc 4141 cccaagcctt tcgggcccca ggtggaggag gagtgctgcc tggagacgca cgttcgaggc 4201 ctgctggagc ccctgggcct cgcctgcacg ttcatcgatg acatctccgc ctaccacaag 4261 ttcctgggag aggttcactg tggcaccaat gtccgcagga agccgttcac cttcaagtgg 4321 tggcacatgg tgcccggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 4381 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 4441 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 4501 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 4561 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 4621 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 4681 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 4741 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 4801 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 4861 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 4921 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 4981 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 5041 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 5101 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 5161 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 5221 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 5281 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 5341 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 5401 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 5461 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 5521 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 5581 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 5641 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 5701 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 5761 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 5821 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 5881 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 5941 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 6001 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 6061 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 6121 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 6181 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 6241 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 6301 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 6361 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 6421 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 6481 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 6541 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 6601 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 6661 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 6721 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 6781 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 6841 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6901 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 6961 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 7021 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 7081 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 7141 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 7201 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 7261 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 7321 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 7381 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 7441 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 7501 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 7561 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 7621 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 7681 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 7741 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 7801 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 7861 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 7921 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7981 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 8041 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 8101 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 8161 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 8221 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 8281 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 8341 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 8401 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 8461 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 8521 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 8581 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 8641 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 8701 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 8761 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 8821 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8881 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8941 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 9001 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 9061 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 9121 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 9181 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 9241 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9301 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9361 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9421 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 9481 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9541 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 9601 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9661 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9721 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9781 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 9841 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 9901 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 9961 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 10021 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 10081 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 10141 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 10201 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 10261 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.