pLKO-H1-2O2-PRMT1-shRNA2-hPGK-

pLKO-H1-2O2-PRMT1-shRNA2-hPGK-TetR-PGK-Puro plasmid

PVT94555

pLKO-H1-2O2-PRMT1-shRNA2-hPGK-TetR-PGK-Puro plasmid Information

Plasmid name: pLKO-H1-2O2-PRMT1(human)-shRNA2-hPGK-TetR-PGK-Puro
Alias: GTGTTCCAGTATCTCTGATTA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence,Tetracycline induction
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: H1-2O2
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: H1
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLKO-H1-2O2-PRMT1-shRNA2-hPGK-TetR-PGK-Puro plasmid Description

pLKO-H1-2O2-PRMT1-shRNA2-hPGK-TetR-PGK-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLKO-H1-2O2-PRMT1-shRNA2-hPGK-TetR-PGK-Puro plasmid Sequence

1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc 301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc 361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc 421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt 481 gaccgaatca ccgacctctc tccccagggg gatctgtgag tttggggacc cttgattgtt 541 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt 601 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct 661 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa 721 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt 781 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat 841 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat 901 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc 961 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga 1021 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt 1081 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat 1141 tgtaatacga ctcactatag ggcgaattga tatgtctaga ttagataaaa gtaaagtgat 1201 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact 1261 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc 1321 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt 1381 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt 1441 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta 1501 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc 1561 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga 1621 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt 1681 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct 1741 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag 1801 cggctcccga taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg 1861 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca 1921 ttccacatcc tccggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc 1981 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac 2041 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca 2101 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg 2161 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg 2221 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc 2281 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc 2341 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 2401 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 2461 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 2521 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 2581 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 2641 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 2701 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 2761 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 2821 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 2881 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 2941 ccgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 3001 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 3061 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 3121 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 3181 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 3241 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 3301 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 3361 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 3421 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 3481 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 3541 gcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 3601 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct 3661 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 3721 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 3781 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 3841 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 3901 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 3961 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 4021 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 4081 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 4141 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 4201 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg 4261 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 4321 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 4381 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 4441 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 4501 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 4561 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 4621 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 4681 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 4741 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 4801 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 4861 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 4921 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 4981 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 5041 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 5101 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 5161 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 5221 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 5281 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 5341 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 5401 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 5461 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 5521 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 5581 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 5641 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 5701 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 5761 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 5821 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 5881 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 5941 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 6001 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 6061 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 6121 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 6181 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 6241 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 6301 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 6361 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 6421 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 6481 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 6541 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 6601 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 6661 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 6721 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 6781 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 6841 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 6901 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 6961 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 7021 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg 7081 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga 7141 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag 7201 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac 7261 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa 7321 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 7381 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 7441 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 7501 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg 7561 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac 7621 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat 7681 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa 7741 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa 7801 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc 7861 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg 7921 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga 7981 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag 8041 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac 8101 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag 8161 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag 8221 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga 8281 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca 8341 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc 8401 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta 8461 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca 8521 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc 8581 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt 8641 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg 8701 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt 8761 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa 8821 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc 8881 gacggtatcg atcacgagac tagcctcgag cggccgcaat atttgcatgt cgctatgtgt 8941 tctgggaaat caccataaac gtgaaatccc tatcagtgat agagacttat aagttcccta 9001 tcagtgatag agacaccggG TGTTCCAGTA TCTCTGATTA ttcaagagaT AATCAGAGAT 9061 ACTGGAACAC TTTTTTgaat tctcgacctc gagacaaatg gcagtattca tccacaattt 9121 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 9181 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt 9241 ttattacagg gacagcagag atccactttg gccgcggctc gaggggg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.