pLV3-CMV-Tmbim1-3×FLAG-CopGFP-

pLV3-CMV-Tmbim1-3×FLAG-CopGFP-Puro plasmid

PVT93394

pLV3-CMV-Tmbim1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Tmbim1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_027154.5;2310061B02Rik; mKIAA4161; RECS1; Tmbib1
Gene lenght: 927bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Tmbim1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Tmbim1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Tmbim1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt ccaatcccag tgccccacct 2341 ccctatgagg accacaaccc cctgtaccct ggctctccac ctcctggtgg ctacgggcag 2401 ccatctgtcc tgccaggtgg ttatcccgcc taccctgcct accctcaacc tggttacggt 2461 catcctgctg gctacccaca gccggttcct cccgtccatc caatgcccat gaactacggc 2521 catgactata atgaagagga gagagcagga agtgacagct ttcgtcctgg agaatgggat 2581 gaccggaaag ttcgacattc cttcatccaa aaggtgtatt gcatcatttc tgtccagctg 2641 ctcatcactg tggccatcat tgcaatcttc acctttgtgg aaccagtcgg caaatacgtg 2701 aggaacaatg tggctgtgta ctacgtgtcc tatgctgtct tccttgtcac ctacctgacc 2761 cttgcctgct gccagggacc caggcgccga ttcccatgga acatcatctt gctcaccatc 2821 tttactttgg ctttgggctt tgtgacaggc actatttcca gtatgtatga aaccaaagct 2881 gtcatcatag caatgatcat caccgctgtg gtatccatct ctgtcaccat cttctgcttt 2941 cagactaagg tggacttcac ctcgtgtaca ggcctcttct gtgtgctggg aatagtgctg 3001 atggtgaccg gaattgtcac aagcatagtg ctgatcttta aatatattta ctggctccac 3061 atggtctatg cagctctggg ggccatctgt ttcaccctgt tcctggctta tgacacacag 3121 ctggtcctgg ggaaccggaa gcacaccatc agcccggagg actacatcac aggcgcccta 3181 cagatctaca cagatatagt ctatatcttc acgttcgtgc tccagctcgt aggaagtaga 3241 gacggatccG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG 3301 GATGACGATG ACAAGtagcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg 3361 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 3421 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 3481 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 3541 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 3601 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 3661 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 3721 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 3781 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 3841 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag 3901 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 3961 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 4021 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 4081 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 4141 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 4201 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 4261 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 4321 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 4381 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 4441 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 4501 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 4561 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 4621 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 4681 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 4741 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 4801 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 4861 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 4921 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 4981 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 5041 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 5101 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 5161 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 5221 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 5281 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 5341 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 5401 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 5461 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 5521 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 5581 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 5641 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa ATCATCGTCC 5701 TTTCCTtggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 5761 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 5821 cctcttccgc gtctTcgcct tcgccctcag acgagtcgga tctccctttg gGccgcctcc 5881 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 5941 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaGataag atctgctttt 6001 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 6061 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 6121 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 6181 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 6241 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 6301 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 6361 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctaTCCCGCC CCTAACTCCG 6421 CCCAtcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 6481 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 6541 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct 6601 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 6661 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 6721 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 6781 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 6841 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6901 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 6961 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 7021 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 7081 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 7141 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 7201 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 7261 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 7321 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 7381 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 7441 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 7501 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 7561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 7621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 7681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 7741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 7801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 7861 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 7921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 7981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 8041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 8101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 8161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 8221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 8281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 8341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 8401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 8461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 8521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 8581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 8641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 8701 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 8761 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 8821 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 8881 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 8941 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 9001 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 9061 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 9121 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 9181 gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.