pCMV-ZNF462-3×FLAG-Neo plasmid

pCMV-ZNF462-3×FLAG-Neo plasmid

PVT93163

pCMV-ZNF462-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-ZNF462(human)-3×FLAG-Neo
Alias: NM_021224.6;WSKA; Zfp462; ZFPIP
Gene lenght: 7518bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ZNF462-3×FLAG-Neo plasmid Description

pCMV-ZNF462-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-ZNF462-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgga ggtgcttcag tgtgatggct 781 gtgatttccg agccccgtct tatgaagatc tcaaggcaca cattcaggat gtccacacgg 841 catttctgca gccaactgat gttgctgagg acaatgtgaa tgagctacga tgtgggtccg 901 tgaatgccag taatcagaca gaggtggagt tttcttctat aaaggatgaa tttgccattg 961 cagaagattt atcaggtcaa aatgcaactt cattggggac cggaggttac tatggccaca 1021 gtccaggata ttatggtcag catattgccg ctaatcccaa accaacaaac aagttttttc 1081 aatgcaagtt ctgtgtacgc tacttcaggt caaaaaacct cctcatagaa cacactagaa 1141 aggtccatgg agctcaagct gaagggagtt catcaggacc ccctgtcccg ggatccttaa 1201 attataatat catgatgcac gagggatttg gaaaggtctt ctcttgccag ttttgcacat 1261 acaagtcacc aagaagggca agaataatta agcatcagaa gatgtatcac aaaaacaatt 1321 tgaaggagac cactgctccc ccacctgctc ctgctccaat gccagaccct gtggttccgc 1381 ccgtatcact gcaggacccc tgcaaggaac tgccagcaga ggttgtggag cgcagcatct 1441 tagagtctat ggtcaagcct ttgaccaaat ctcgaggcaa cttttgttgt gagtggtgca 1501 gctaccagac cccccgccga gaacgctggt gtgaccacat gatgaagaaa caccgcagta 1561 tggtcaagat cctttccagt ctcagacagc aacaagaagg aactaatcta cctgatgtgc 1621 cgaacaagag tgcccccagc cccacttcca actccaccta tctgaccatg aatgctgcaa 1681 gccgggagat acccaatact accgtctcca acttcagggg ctccatgggc aactccatca 1741 tgagacccaa ttcttcagct tccaagtttt cgcccatgtc ttaccctcag atgaagccga 1801 agtcacctca caattctggt ctagttaact tgacagagag atcccgttat ggaatgactg 1861 acatgaccaa ttcttctgct gacctggaaa ctaacagcat gctaaatgac tctagttctg 1921 atgaagagtt aaatgaaata gacagtgaga atggtttaag tgctatggat caccagacat 1981 caggcctgtc tgcagagcag ctgatgggct cagatggcaa caaattattg gagaccaagg 2041 ggattccatt tagaagattc atgaataggt tccagtgccc cttttgtcct ttcctcacca 2101 tgcatcgacg tagcatctct cgtcacatag aaaacatcca cttatctgga aagacagctg 2161 tctacaaatg tgacgaatgt ccgtttactt gcaagagctc gttgaaactt ggggctcaca 2221 aacagtgtca cacgggtaca acgtcagatt gggatgctgt gaattcccag agtgaaagca 2281 tttcttcctc actgaatgaa ggtgtggtgt cttatgagag ctcaagcatc aatggtagaa 2341 agtcaggagt catgttggat cccttgcagc agcaacagcc accgcagcca ccaccaccgc 2401 cgccgccacc accaccatca cagccacagc cactgcagca gccacagcca ccacagctgc 2461 agccaccaca tcaggtgcca ccccagccac aaacacagcc accaccaacg cagcagccac 2521 agccacccac acaagccgca cctctgcacc catacaaatg caccatgtgt aattactcca 2581 ccacaactct gaaagggcta agagtccatc agcagcataa acattcattc tgtgacaact 2641 tgccaaaatt cgaggggcag ccctcaagcc taccattgga aaatgagaca gacagccacc 2701 cctcttccag caacactgtg aagaaaagtc agacctcaat tcttgggttg tcctccaaga 2761 acaattttgt agctaaagcc tctaggaagc tcgccaatga ctttcctcta gatttgtcac 2821 ccgtgaagaa gagaaccagg attgacgaga tagcaagcaa ccttcagagc aaaattaacc 2881 aaaccaaaca gcaggaagat gcagtgatca atgttgagga tgatgaagag gaagaggaag 2941 acaacgaagt cgagatagag gttgagttgg acagggagga agaaccgaca gaacccatca 3001 tagaggttcc cacttccttt tctgcccaac agatatgggt aagagatacc agtgagcccc 3061 agaaagagcc caacttcaga aacatcaccc acgattacaa tgccaccaat ggggctgaga 3121 ttgagctcac cctttctgaa gatgaagagg attattatgg ctcctcaaca aacttgaaag 3181 atcaccaagt ttccaatact gctctgctga atacccaaac tcccatctat gggactgagc 3241 acaatagtga aaacacagac tttggtgact ctggaaggct ttactattgt aaacactgtg 3301 actttaacaa caaatctgcc cggagtgtta gcacccacta ccaacgaatg cacccataca 3361 ttaaattcag ctttaggtac atcttggacc ccaatgatca cagtgcagtg tacaggtgcc 3421 tggaatgcta catcgattac accaacttcg aagatctcca gcagcattat ggcgagcacc 3481 acccagaagc catgaatgta ctcaactttg atcactcgga cctgatctac cggtgtcggt 3541 tttgttcata cacgagcccg aatgttagaa gcctgatgcc acattaccaa agaatgcatc 3601 ccacggtgaa gatcaacaac gcgatgatat tttcaagcta tgtcgtggag cagcaggaag 3661 ggctgaatac agaatcccag accctgaggg agattctgaa ttcggctccc aagaacatgg 3721 cgacttccac acctgtggct cgtggtggtg gtttgccagc tacgttcaac aaaaacactc 3781 ctaagacctt tactcctgaa tgtgaaaatc agaaggaccc tttggtcaac actgttgttg 3841 tttatgattg tgatgtttgt tcgtttgcaa gccccaacat gcattctgtc ttggttcatt 3901 atcagaagaa acaccccgaa gaaaaggctt cctactttag gatccagaaa actatgcgaa 3961 tggtgtctgt ggacaggggc tctgcccttt ctcaattatc atttgaggtg ggtgctccaa 4021 tgtctcccaa aatgtccaac atgggttccc cacccccccc acaacccccg ccaccagacc 4081 tcagtactga gctttactac tgcaaacact gttcctacag caatcggtca gttgtgggag 4141 tgcttgtcca ctaccagaaa agacacccag aaataaaggt tactgccaaa tatatcagac 4201 aggctcctcc cacagctgca atgatgagag gggtcgaagg gccccaaggc tccccccggc 4261 cacccgcccc catacaacag ctgaaccgaa gcagctctga gagagatggc cctcctgtgg 4321 agaatgagat gttcttttgc cagcactgtg attatgggaa ccggacggtc aaaggggtac 4381 tcattcatta tcagaagaag caccgagact tcaaggccaa tgcagatgtg atccggcagc 4441 atacggccac cattcgaagc ctctgcgacc gaaatcagaa gaagcctgcc agctgcgtgc 4501 ttgtctcccc ctctaatctg gagcgggaca aaacgaaact ccgagcactc aaatgtaggc 4561 agtgctcata tacctccccc tacttctatg cactgaggaa gcatatcaag aaagaccacc 4621 ccgccctgaa agccacagtc acgtccatca tgcgatgggc atttctagat ggcttgatag 4681 aagctggcta ccactgcgag tggtgcatct actcccatac ggagcccaac ggtttgctcc 4741 tgcattacca acggaggcat ccagaacact atgttgatta cacctacatg gctactaaac 4801 tgtgggctgg gccagaccca tcccctccct ctctcacaat gccagccgaa gccaaaacct 4861 acagatgcag ggactgtgtt ttcgaagctg tttccatctg ggacatcact aatcactacc 4921 aagcattcca cccctgggcc atgaatggtg atgagtcagt gctactggac atcatcaagg 4981 agaaagatgc tgtggagaag cccattcttt catccgaaga gttggcaggc cctgtgaatt 5041 gtgaaaacag tatacccacc cctttcccgg agcaggaagc tgaatgtcca gaggatgcaa 5101 gactgtcccc tgagaaaagc ctgcagctag cttcagccaa ccccgccata tcctccaccc 5161 cataccagtg cacggtatgc caatctgagt ataacaactt gcacggcctt ctcactcatt 5221 atgggaagaa gcaccctggc atgaaagtga aggctgctga ctttgcccag gacattgaca 5281 tcaacccagg tgccgtctac aaatgcaggc attgcccata catcaacacc cgcatccacg 5341 gcgtactgac ccactaccag aagcgacacc cgtccatcaa ggtgaccgct gaggactttg 5401 tgcacgacgt agagcagtct gctgacatat cccagaatga cgtggaggag acgagcagga 5461 tcttcaagca agggtatggc gcctaccggt gcaaactgtg tccgtacaca cacggcactt 5521 tggagaaact aaaaatccac tacgagaagt atcacaatca gcctgaattt gatgtctttt 5581 cccagtcgcc cccgaagctg ccagtccccc tcgagcccga gatgaccact gaagtgagcc 5641 cttcccaagt ctccatcact gaggaggagg tgggagagga gcccgtgtcc acttctcact 5701 tctctacctc ccacctggtc tcccacactg tgttccggtg ccagctctgc aagtacttct 5761 gctccacgag gaaggggatc gccaggcact accgcatcaa gcacaataat gtccgagccc 5821 agccagaagg caagaacaac ctcttcaagt gtgccctgtg tgcctacacc aaccccatcc 5881 gcaaaggtct ggcagcccac taccagaagc gccacgacat tgatgcgtat tacactcact 5941 gcttggcagc ctccaggacc atcagcgaca agcccaacaa agtgatcatc ccatccccgc 6001 ccaaggacga ctcccctcag ctgagcgagg aactccggcg ggcagtggag aagaaaaagt 6061 gctccttgtg ctctttccag tcgttcagca agaagggcat cgtgtcccat tacatgaaac 6121 gccacccagg ggtgttccca aagaagcagc acgccagcaa gttggggggc tacttcacgg 6181 ccgtctatgc agatgagcat gagaagccca cactgatgga agaagaggag agaggcaact 6241 ttgagaaagc cgaggtggag ggtgaagctc aggaaatcga gtggctccca ttccgctgca 6301 tcaaatgctt caagctgtcc tttagcactg cagagctgct gtgcatgcat tacactgacc 6361 accacagtcg ggacctaaag agggacttca tcattctggg caacggcccc cgcttgcaga 6421 actccaccta ccagtgtaag cactgtgata gcaaactgca aagcacagcc gagctgacct 6481 cacacttgaa cattcacaat gaggaattcc agaagcgtgc caaacgtcag gagaggagga 6541 aacagctttt gagcaagcag aaatatgcag atggtgcttt tgcagatttc aaacaagaga 6601 ggccttttgg tcacttagaa gaggtgccaa agatcaagga gaggaaagtg gtgggctaca 6661 aatgtaaatt ctgtgtggaa gtgcacccaa cgctccgagc catctgcaat cacctccgaa 6721 agcacgtcca gtatggcaat gtcccagctg tgtcagctgc tgtgaagggg ctgcgttctc 6781 atgagaggag ccacctggcc ctggccatgt ttacccgcga ggacaagtac agctgccagt 6841 attgctcgtt tgtttctgct ttcaggcaca atttggatcg ccatatgcaa acccaccacg 6901 gacaccataa accattccga tgcaaactct gctccttcaa gtcctcctat aacagccggc 6961 tgaaaacaca tatactcaaa gctcatgctg gtgagcatgc ctacaagtgt tcttggtgct 7021 cattctccac catgacaatc agccagctga aggaacactc cctcaaggtc cacggaaaag 7081 ccctgaccct ccccaggcca cggatcgtca gtctcctctc ctcacactcc caccactcct 7141 cccaaaaagc taccccggct gaagaagtgg aagactccaa tgactcatca tattcagagc 7201 ccccagatgt tcagcagcag ttgaaccact atcagtcagc tgccctggca aggaacaaca 7261 gccgtgttag ccctgtgcct ctttctgggg ctgctgctgg cactgagcag aaaactgaag 7321 ccgtgcttca ctgcgaattc tgtgaattct cctccggcta catccagagc atcaggcgtc 7381 attaccggga caagcatggt gggaagaagc ttttcaagtg caaagactgc tccttttaca 7441 caggctttaa atctgctttt actatgcacg tggaagctgg gcactcagca gttcccgagg 7501 agggccccaa agatcttcgc tgtcctctct gcctctatca caccaaatac aagcgcaaca 7561 tgattgacca catcgtgctg caccgagaag agcgtgttgt ccccattgaa gtttgccggt 7621 ccaaactgtc caaatacttg cagggagtag ttttccgctg tgataagtgt accttcacct 7681 gctccagtga tgagagcctc cagcaacata tagaaaagca caatgaactg aaaccttaca 7741 aatgccagct ctgctactat gagaccaagc acacggagga actggacagc caccttcggg 7801 atgagcataa ggtaagccgt aactttgagc tggttggacg ggttaacttg gatcagctgg 7861 aacagatgaa ggagaaaatg gagagctcca gcagcgatga tgaggacaag gaagaagaaa 7921 tgaacagcaa ggctgaagac agagagctga tgagattttc tgaccacggg gctgctctta 7981 acactgagaa gcgttttcca tgtgaatttt gtggacgggc gttttcacag ggctctgagt 8041 gggaaagaca tgtgctgaga cacggcatgg cattgaatga caccaagcag gtgagcagag 8101 aagaaatcca cccaaaagag atcatggaga acagtgttaa aatgccctcc atagaggaaa 8161 aggaagatga cgaggccatt gggatagact tttccctaaa gaatgaaaca gtagccatct 8221 gtgtagtaac tgccgacaaa tctctcctgg agaatgcaga ggccaaaaaa gaaGGATCCG 8281 ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG 8341 ACAAGTAGTG AGGGCCCGAT ATCTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG 8401 ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT 8461 GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC 8521 CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG 8581 CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT 8641 TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT 8701 TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG 8761 GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC 8821 GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT 8881 GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT 8941 TTGGGCCGCC TCCCCGCCTG GAAACGGGGG AGGCTAACTG AAACACGGAA GGAGACAATA 9001 CCGGAAGGAA CCCGCGCTAT GACGGCAATA AAAAGACAGA ATAAAACGCA CGGGTGTTGG 9061 GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC 9121 GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG 9181 TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCAGA 9241 TCTGCGCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC 9301 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC 9361 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT 9421 AAATCGGGGC ATCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA 9481 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC 9541 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT 9601 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGGGGATTT CGGCCTATTG 9661 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT 9721 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 9781 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 9841 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 9901 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 9961 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 10021 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 10081 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 10141 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 10201 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 10261 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 10321 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 10381 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 10441 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 10501 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 10561 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 10621 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 10681 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 10741 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 10801 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 10861 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 10921 TCGCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC 10981 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 11041 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA 11101 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 11161 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC 11221 GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 11281 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 11341 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 11401 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 11461 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 11521 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 11581 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 11641 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 11701 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 11761 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 11821 CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 11881 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 11941 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 12001 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 12061 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT 12121 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 12181 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 12241 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 12301 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 12361 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 12421 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 12481 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 12541 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 12601 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 12661 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 12721 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 12781 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 12841 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 12901 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 12961 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 13021 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 13081 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 13141 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 13201 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 13261 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 13321 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 13381 ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA GATCTCCCGA TCCCCTATGG 13441 TCGACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATCT GCTCCCTGCT 13501 TGTGTGTTGG AGGTCGCTGA GTAGTGCGCG AGCAAAATTT AAGCTACAAC AAGGCAAGGC 13561 TTGACCGACA ATTGCATGAA GAATCTGCTT AGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.