pCMV-GABBR2-FLAG-TwinStrep-Neo

pCMV-GABBR2-FLAG-TwinStrep-Neo plasmid

PVT93047

pCMV-GABBR2-FLAG-TwinStrep-Neo plasmid Information

Plasmid name: pCMV-GABBR2(human)-FLAG-TwinStrep-Neo
Alias: NM_005458.8;DEE59; EIEE59; GABABR2; GPR51; GPRC3B;HG20; HRIHFB2099; NDPLHS
Gene lenght: 2823bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-GABBR2-FLAG-TwinStrep-Neo plasmid Description

pCMV-GABBR2-FLAG-TwinStrep-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-GABBR2-FLAG-TwinStrep-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTGAATT CGCCACCatg gcttccccgc ggagctccgg gcagcccggg 961 ccgccgccgc cgccgccacc gccgcccgcg cgcctgctac tgctactgct gctgccgctg 1021 ctgctgcctc tggcgcccgg ggcctggggc tgggcgcggg gcgccccccg gccgccgccc 1081 agcagcccgc cgctctccat catgggcctc atgccgctca ccaaggaggt ggccaagggc 1141 agcatcgggc gcggtgtgct ccccgccgtg gaactggcca tcgagcagat ccgcaacgag 1201 tcactcctgc gcccctactt cctcgacctg cggctctatg acacggagtg cgacaacgca 1261 aaagggttga aagccttcta cgatgcaata aaatacgggc ctaaccactt gatggtgttt 1321 ggaggcgtct gtccatccgt cacatccatc attgcagagt ccctccaagg ctggaatctg 1381 gtgcagcttt cttttgctgc aaccacgcct gttctagccg ataagaaaaa atacccttat 1441 ttctttcgga ccgtcccatc agacaatgcg gtgaatccag ccattctgaa gttgctcaag 1501 cactaccagt ggaagcgcgt gggcacgctg acgcaagacg ttcagaggtt ctctgaggtg 1561 cggaatgacc tgactggagt tctgtatggc gaggacattg agatttcaga caccgagagc 1621 ttctccaacg atccctgtac cagtgtcaaa aagctgaagg ggaatgatgt gcggatcatc 1681 cttggccagt ttgaccagaa tatggcagca aaagtgttct gttgtgcata cgaggagaac 1741 atgtatggta gtaaatatca gtggatcatt ccgggctggt acgagccttc ttggtgggag 1801 caggtgcaca cggaagccaa ctcatcccgc tgcctccgga agaatctgct tgctgccatg 1861 gagggctaca ttggcgtgga tttcgagccc ctgagctcca agcagatcaa gaccatctca 1921 ggaaagactc cacagcagta tgagagagag tacaacaaca agcggtcagg cgtggggccc 1981 agcaagttcc acgggtacgc ctacgatggc atctgggtca tcgccaagac actgcagagg 2041 gccatggaga cactgcatgc cagcagccgg caccagcgga tccaggactt caactacacg 2101 gaccacacgc tgggcaggat catcctcaat gccatgaacg agaccaactt cttcggggtc 2161 acgggtcaag ttgtattccg gaatggggag agaatgggga ccattaaatt tactcaattt 2221 caagacagca gggaggtgaa ggtgggagag tacaacgctg tggccgacac actggagatc 2281 atcaatgaca ccatcaggtt ccaaggatcc gaaccaccaa aagacaagac catcatcctg 2341 gagcagctgc ggaagatctc cctacctctc tacagcatcc tctctgccct caccatcctc 2401 gggatgatca tggccagtgc ttttctcttc ttcaacatca agaaccggaa tcagaagctc 2461 ataaagatgt cgagtccata catgaacaac cttatcatcc ttggagggat gctctcctat 2521 gcttccatat ttctctttgg ccttgatgga tcctttgtct ctgaaaagac ctttgaaaca 2581 ctttgcaccg tcaggacctg gattctcacc gtgggctaca cgaccgcttt tggggccatg 2641 tttgcaaaga cctggagagt ccacgccatc ttcaaaaatg tgaaaatgaa gaagaagatc 2701 atcaaggacc agaaactgct tgtgatcgtg gggggcatgc tgctgatcga cctgtgtatc 2761 ctgatctgct ggcaggctgt ggaccccctg cgaaggacag tggagaagta cagcatggag 2821 ccggacccag caggacggga tatctccatc cgccctctcc tggagcactg tgagaacacc 2881 catatgacca tctggcttgg catcgtctat gcctacaagg gacttctcat gttgttcggt 2941 tgtttcttag cttgggagac ccgcaacgtc agcatccccg cactcaacga cagcaagtac 3001 atcgggatga gtgtctacaa cgtggggatc atgtgcatca tcggggccgc tgtctccttc 3061 ctgacccggg accagcccaa tgtgcagttc tgcatcgtgg ctctggtcat catcttctgc 3121 agcaccatca ccctctgcct ggtattcgtg ccgaagctca tcaccctgag aacaaaccca 3181 gatgcagcaa cgcagaacag gcgattccag ttcactcaga atcagaagaa agaagattct 3241 aaaacgtcca cctcggtcac cagtgtgaac caagccagca catcccgcct ggagggccta 3301 cagtcagaaa accatcgcct gcgaatgaag atcacagagc tggataaaga cttggaagag 3361 gtcaccatgc agctgcagga cacaccagaa aagaccacct acattaaaca gaaccactac 3421 caagagctca atgacatcct caacctggga aacttcactg agagcacaga tggaggaaag 3481 gccattttaa aaaatcacct cgatcaaaat ccccagctac agtggaacac aacagagccc 3541 tctcgaacat gcaaagatcc tatagaagat ataaactctc cagaacacat ccagcgtcgg 3601 ctgtccctcc agctccccat cctccaccac gcctacctcc catccatcgg aggcgtggac 3661 gccagctgtg tcagcccctg cgtcagcccc accgccagcc cccgccacag acatgtgcca 3721 ccctccttcc gagtcatggt ctcgggcctg CTCGAGGATT ACAAGGATGA CGACGATAAG 3781 GGATCCagcg cctggtccca ccctcagttt gagaagggcg gaggctctgg cggcggaagc 3841 ggaggatctg cttggagcca cccccagttc gaaaagtgaT GAGGGCCCGT TTAAACCCGC 3901 TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG 3961 CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT 4021 GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC 4081 AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT 4141 TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC 4201 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 4261 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 4321 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 4381 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 4441 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 4501 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 4561 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT 4621 GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 4681 AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 4741 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC 4801 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA 4861 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT 4921 GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA 4981 TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT 5041 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC 5101 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC 5161 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC 5221 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG 5281 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC 5341 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG 5401 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC 5461 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC 5521 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA 5581 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA 5641 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG 5701 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG 5761 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG 5821 GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA 5881 TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG 5941 GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT 6001 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT 6061 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG 6121 TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 6181 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 6241 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 6301 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 6361 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 6421 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 6481 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 6541 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 6601 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 6661 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 6721 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 6781 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 6841 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 6901 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 6961 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 7021 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 7081 AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 7141 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 7201 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 7261 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 7321 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 7381 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 7441 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 7501 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 7561 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 7621 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 7681 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 7741 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 7801 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 7861 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 7921 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 7981 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 8041 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 8101 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 8161 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 8221 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 8281 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.