pLO5-ciZZQ2 plasmid

pLO5-ciZZQ2 plasmid

PVT93028

pLO5-ciZZQ2 plasmid Information

Plasmid name: pLO5-ciZZQ2
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pLO5-ciZZQ2 plasmid Description

pLO5-ciZZQ2 is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLO5-ciZZQ2 plasmid Sequence

1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa 61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga 121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca 181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac 241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa 301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag 361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct 421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg 481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg 541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct 601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa 781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag 841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga 901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa 961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag 1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag 1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt 1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa 1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc 1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat 1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa 1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg 1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat 1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc 1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg 1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt 1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga 1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact 1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag 1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat 1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta 1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc 2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca 2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca 2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 2281 tcgggtttat tacagggaca gcagagatcc actttggatc gataagcttt gcaaagatgg 2341 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga 2401 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 2461 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac 2521 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 2581 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 2641 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg 2701 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga 2761 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt 2821 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt 2881 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt 2941 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt 3001 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg 3061 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct 3121 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg 3181 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca 3241 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg 3301 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg 3361 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt 3421 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac 3481 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag 3541 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagG AATTGctcca 3601 cctcccaggt tcaagcgatt ctcctccctc agcctcccga gtagctggga ccacaggcat 3661 gcaccaccat ccccagctaa tttttgcatt attagtagag ttgggatttc ttcaccgtgt 3721 tggccaggcC ggtcttggac tcctgacctc aagtgatcca actgcctcag cctctcaaag 3781 tgctaggatt acaggGATCT ATACTTTTCT GATATTATAA AGATAGTTAT CTTCTCCAAG 3841 GGAAAAAATC ATCTTCATGG AAATTAATTA CTTTTTTACA AATTGTGAAT TTGACCCTTA 3901 AGAGTTTTCT TCCTGATATT TAAAATTGAA AAAAAAATTG TTGACATTAA TAtttcttct 3961 ttcGAATTCT AATACtttcA GCTGAGCTTC AGTCATTGTC TATTCCTGGA ACTTACCAAG 4021 AGAAGATCAC CCACCTGGGA CATTCTCTGA TGAGTTTAAC AGGTCTGAAA TCTTTGGATC 4081 TCTCGCGCAA CTCCTTGGTT AGTCTGGAGG GCATTCAGTA CCTGACTGCA TTGGAGAGTC 4141 TCAATCTCTA CTACAACTGC ATCTCCTCGT TGGCAGAAGT GTTTCGGCTC CACGCCTTAA 4201 CCGAGCTCGT GGATGTGGAC TTCCGGCTGA ACCCCGTGGT GAAGGTTGAG CCTGACTACC 4261 GCCTTTTTGT TGTGCACCTG CTCCCCAAGC TCCAGCAGCT GGGTAAGAAC AACTGGATCC 4321 TAGCTAACAA CTCCATACTT TTTGGTTGTT TATTAATGTG AAATTTCTGC TAAATGAAAT 4381 ACTTTTGTGT GTGTTTGTGG TAGAAGAGAC CACTTCAGTT AAATAAGGAA ATCAAGAGAG 4441 GATCAATTTA GGAAGATTCA GATATACAgc cgggtgcagt ggctcatgcc tgtaatccct 4501 gcacttaggg aggctgaggc gggtggatga cctgaggtta ggagttcaag accagcctgg 4561 ccaacatggc gaaaccccca tctctactaa aaataacaaa aattagctgg gtgtggtggt 4621 gggtgtctat aatcccagca acttgggagg ctgaggcagg agaatcacat gcatctaggg 4681 cggccaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga 4741 ataaggccgg tgtgcgtttg tctatatgtg attttccacc atattgccgt cttttggcaa 4801 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc 4861 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc 4921 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg 4981 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca 5041 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag 5101 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct 5161 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc 5221 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc 5281 acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 5341 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 5401 cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 5461 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 5521 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5581 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5641 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5701 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5761 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5821 ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc 5881 cgcaagcccg gtgcctgata ataggcggcc gctcgagacc tagaaaaaca tggagcaatc 5941 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag 6001 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca 6061 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 6121 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 6181 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 6241 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 6301 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 6361 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattataat 6421 agatttagca ggaattgaac taggagtgga gcacacaggc aaagttctag agctcgctga 6481 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 6541 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 6601 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 6661 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 6721 gaggcggaaa gaaccagctg ggggcgcgcc cctcgaggcc gccatggtca tagctgtttg 6781 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 6841 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 6901 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 6961 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 7021 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 7081 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 7141 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 7201 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 7261 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 7321 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 7381 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 7441 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 7501 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 7561 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 7621 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 7681 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 7741 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 7801 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 7861 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 7921 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 7981 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 8041 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 8101 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 8161 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 8221 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 8281 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat 8341 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 8401 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 8461 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 8521 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.