pCMV-GPRC6A-FLAG-TwinStrep-Neo

pCMV-GPRC6A-FLAG-TwinStrep-Neo plasmid

PVT92997

pCMV-GPRC6A-FLAG-TwinStrep-Neo plasmid Information

Plasmid name: pCMV-GPRC6A(human)-FLAG-TwinStrep-Neo
Alias: NM_148963.4;bA86F4.3; GPCR
Gene lenght: 2778bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-GPRC6A-FLAG-TwinStrep-Neo plasmid Description

pCMV-GPRC6A-FLAG-TwinStrep-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-GPRC6A-FLAG-TwinStrep-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTGAATT CGCCACCatg gcattcttaa ttatactaat tacctgcttt 961 gtgattattc ttgctacttc acagccttgc cagacccctg atgactttgt ggctgccact 1021 tctccgggac atatcataat tggaggtttg tttgctattc atgaaaaaat gttgtcctca 1081 gaagactctc ccagacgacc acaaatccag gagtgtgttg gctttgaaat atcagttttt 1141 cttcaaactc ttgccatgat acacagcatt gagatgatca acaattcaac actcttacct 1201 ggagtcaaac tggggtatga aatctatgac acttgtacag aagtcacagt ggcaatggca 1261 gccactctga ggtttctttc taaattcaac tgctccagag aaactgtgga gtttaagtgt 1321 gactattcca gctacatgcc aagagttaag gctgtcatag gttctgggta ctcagaaata 1381 actatggctg tctccaggat gttgaattta cagctcatgc cacaggtggg ttatgaatca 1441 actgcagaaa tcctgagtga caaaattcgc tttccttcat ttttacggac tgtgcccagt 1501 gacttccatc aaattaaagc aatggctcac ctgattcaga aatctggttg gaactggatt 1561 ggcatcataa ccacagatga tgactatgga cgattggctc ttaacacttt tataattcag 1621 gctgaagcaa ataacgtgtg catagccttc aaagaggttc ttccagcctt tctttcagat 1681 aataccattg aagtcagaat caatcggaca ctgaagaaaa tcattttaga agcccaggtt 1741 aatgtcattg tggtatttct gaggcaattc catgtttttg atctcttcaa taaagccatt 1801 gaaatgaata taaataagat gtggattgct agtgataatt ggtcaactgc caccaagatt 1861 accaccattc ctaatgttaa aaagattggc aaagttgtag ggtttgcctt tagaagaggg 1921 aatatatcct ctttccattc ctttcttcaa aatctgcact tgcttcccag tgacagtcac 1981 aaactcttac atgaatatgc catgcattta tctgcctgcg catatgtcaa ggacactgat 2041 ttgagtcaat gcatattcaa tcattctcaa aggactttgg cctacaaggc taacaaggct 2101 atagaaagga acttcgtcat gagaaatgac ttcctctggg actatgctga gccaggactc 2161 attcatagta ttcagcttgc agtgtttgcc cttggttatg ccattcggga tctgtgtcaa 2221 gctcgtgact gtcagaaccc caacgccttt caaccatggg agttacttgg tgtgctaaaa 2281 aatgtgacat tcactgatgg atggaattca tttcattttg atgctcacgg ggatttaaat 2341 actggatatg atgttgtgct ctggaaggag atcaatggac acatgactgt cactaagatg 2401 gcagaatatg acctacagaa tgatgtcttc atcatcccag atcaggaaac aaaaaatgag 2461 ttcaggaatc ttaagcaaat tcaatctaaa tgctccaagg aatgcagtcc tgggcaaatg 2521 aagaaaacta caagaagtca acacatctgt tgctatgaat gtcagaactg tcctgaaaat 2581 cattacacta atcagacaga tatgcctcac tgccttttat gcaacaacaa aactcactgg 2641 gcccctgtta ggagcactat gtgctttgaa aaggaagtgg aatatctcaa ctggaatgac 2701 tccttggcca tcctactcct gattctctcc ctactgggaa tcatatttgt tctggttgtt 2761 ggcataatat ttacaagaaa cctgaacaca cctgttgtga aatcatccgg gggattaaga 2821 gtctgctatg tgatccttct ctgtcatttc ctcaattttg ccagcacgag ctttttcatt 2881 ggagaaccac aagacttcac atgtaaaacc aggcagacaa tgtttggagt gagctttact 2941 ctttgcatct cctgcatttt gacgaagtct ctgaaaattt tgctagcctt cagctttgat 3001 cccaaattac agaaatttct gaagtgcctc tatagaccga tccttattat cttcacttgc 3061 acgggcatcc aggttgtcat ttgcacactc tggctaatct ttgcagcacc tactgtagag 3121 gtgaatgtct ccttgcccag agtcatcatc ctggagtgtg aggagggatc catacttgca 3181 tttggcacca tgctgggcta cattgccatc ctggccttca tttgcttcat atttgctttc 3241 aaaggcaaat atgagaatta caatgaagcc aaattcatta catttggcat gctcatttac 3301 ttcatagctt ggatcacatt catccctatc tatgctacca catttggcaa atatgtacca 3361 gctgtggaga ttattgtcat attaatatct aactatggaa tcctgtattg cacattcatc 3421 cccaaatgct atgttattat ttgtaagcaa gagattaaca caaagtctgc ctttctcaag 3481 atgatctaca gttattcttc ccatagtgtg agcagcattg ccctgagtcc tgcttcactg 3541 gactccatga gcggcaatgt cacaatgacc aatcccagct ctagtggcaa gtctgcaacc 3601 tggcagaaaa gcaaagatct tcaggcacaa gcatttgcac acatatgcag ggaaaatgcc 3661 acaagtgtat ctaaaacttt gcctcgaaaa agaatgtcaa gtataCTCGA GGATTACAAG 3721 GATGACGACG ATAAGGGATC Cagcgcctgg tcccaccctc agtttgagaa gggcggaggc 3781 tctggcggcg gaagcggagg atctgcttgg agccaccccc agttcgaaaa gtgaTGAGGG 3841 CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT 3901 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA 3961 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG 4021 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG 4081 GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC 4141 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 4201 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 4261 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 4321 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 4381 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 4441 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 4501 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 4561 GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG 4621 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC 4681 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG 4741 TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC 4801 CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC 4861 TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG 4921 GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA 4981 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT 5041 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC 5101 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG 5161 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG 5221 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC 5281 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC 5341 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG 5401 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC 5461 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG 5521 AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC 5581 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG 5641 CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG 5701 TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG 5761 AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC 5821 ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT 5881 CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA 5941 CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT 6001 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT 6061 ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA 6121 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 6181 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 6241 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA 6301 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 6361 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 6421 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 6481 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 6541 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 6601 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 6661 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 6721 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 6781 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 6841 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 6901 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 6961 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 7021 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC 7081 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 7141 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 7201 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 7261 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 7321 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 7381 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 7441 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 7501 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 7561 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 7621 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 7681 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 7741 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 7801 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 7861 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 7921 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 7981 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 8041 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 8101 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 8161 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 8221 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.