pLV3-CMV-Slc38a1-3×FLAG-CopGFP

pLV3-CMV-Slc38a1-3×FLAG-CopGFP-Puro plasmid

PVT92731

pLV3-CMV-Slc38a1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Slc38a1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_134086.4;Gm32457; NAT2; SNAT1
Gene lenght: 1455bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Slc38a1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Slc38a1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Slc38a1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga tgcatttcaa aagtggcctc 2341 gagctaacgg agctgcagaa catgacggtg cccgaagatg acaacgtcag caacgactct 2401 aatgacttca cagaagtaga aaacggccag ataaatagca agttcatctc agatcgggag 2461 agtaggagga gtctcacaaa cagccatctg gagaaaagga aatgcgacga gtatattcca 2521 ggaacaacct ccctgggaat gtctgtcttt aacctgagca acgccatcat gggcagtgga 2581 atcttgggcc ttgccttcgc cttggccaac accgggatcc tcctctttct gatacttctg 2641 acttcggtga cactgctgtc tatatattca ataaacctac tgctgatctg ttccaaggaa 2701 accggctgca tggtgtatga gaagctggga gagcaagtct tcggcaccac ggggaagctg 2761 gtgatcttcg gagccacctc tctgcagaac actggggcga tgctgagcta cctcttcata 2821 gtaaaaaacg aacttccttc agccataaaa tccctcatgg gagaggagga tgcattttca 2881 gcctggtacg tcgatggccg agtgttggtg gtgatggtga cctttggcat aatccttccg 2941 ctgtgtctct tgaagaacct ggggtatctt ggctacacga gtgggttttc cctaagctgc 3001 atgatgtttt tcctaatagt ggtcatctac aagaaatttc agactccctg catgagtgtg 3061 gagcagaact cgacagtcag tgctaatgtg accgacgcgt gcacaccaaa gtatgtcacc 3121 ttcaactcaa agacggtgta tgctttacca accatcgcct tcgcgtttgt ctgccacccg 3181 tccgtcctgc caatctacag cgagctcaaa gaccggtcac agaagaaaat gcagatggtt 3241 tccaacatct ccttctttgc tatgtttgtc atgtacttcc tgacggccat ctttgggtac 3301 ttgacgtttt atgaaaaggt gcagtcagat ctccttcaca agtaccagag cacaggcgac 3361 attctcatcc taaccgtgcg gctggccgtc atcgtggccg tcatcctcac cgtgcctgtg 3421 ctgtttttca cagttcgatc atcgttattt gagctggcca agaagacaaa gttccacctg 3481 tgccgccatg tcttggtgac catcatactc ttgatcatta tcaacctgtt ggtgatcttc 3541 atcccctcca tgaaggatat ctttggagtc gtaggagtca cgtctgctaa tatgctcatt 3601 ttcattcttc cttcgtctct ttatttaaaa atcacgaacc aggacggaga taaaggcact 3661 caaagaattt gggcggccct tttcttgggc ttgggggtgt tgttctcctt gatcagcatc 3721 cctctggtca tctacgactg ggcttgctcc tctggcaccg acgaaggcca cggatccGAC 3781 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 3841 AAGtagcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca 3901 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga 3961 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 4021 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 4081 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc 4141 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt 4201 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc 4261 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg 4321 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc 4381 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc 4441 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg 4501 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa 4561 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac 4621 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc 4681 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc 4741 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc 4801 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg 4861 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac 4921 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc 4981 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc 5041 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc 5101 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc 5161 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 5221 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 5281 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 5341 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 5401 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 5461 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 5521 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 5581 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 5641 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 5701 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 5761 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 5821 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 5881 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 5941 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 6001 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 6061 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 6121 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 6181 ctgttgggca ctgacaattc cgtggtgttg tcggggaaAT CATCGTCCTT TCCTtggctg 6241 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 6301 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 6361 ctTcgccttc gccctcagac gagtcggatc tccctttggG ccgcctcccc gcctggtacc 6421 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 6481 gggactggaa gggctaattc actcccaacg aaGataagat ctgctttttg cttgtactgg 6541 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 6601 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 6661 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 6721 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 6781 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 6841 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 6901 caatgtatct tatcatgtct ggctctagct aTCCCGCCCC TAACTCCGCC CAtcccgccc 6961 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 7021 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 7081 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg 7141 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 7201 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 7261 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 7321 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 7381 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 7441 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 7501 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 7561 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 7621 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 7681 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 7741 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7801 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7861 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7921 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 7981 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 8041 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 8101 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 8161 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 8221 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 8281 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 8341 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 8401 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 8461 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 8521 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 8581 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 8641 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 8701 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 8761 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 8821 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 8881 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 8941 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 9001 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 9061 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 9121 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 9181 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 9241 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 9301 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 9361 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 9421 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 9481 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 9541 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 9601 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 9661 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 9721 taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.