pBiFC-HA-HMGB2-VC155 plasmid

pBiFC-HA-HMGB2-VC155 plasmid

PVT92723

pBiFC-HA-HMGB2-VC155 plasmid Information

Plasmid name: pBiFC-HA-HMGB2(human)-VC155
Alias: NM_001130688.1;HMG2
Gene lenght: 627bp
Host: Mammalian cells
Use(s): Bimolecular fluorescence
Fragment type: BIFC
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: SV40 intron-F1
Reverse primer:
Copy number: High
Induction:

pBiFC-HA-HMGB2-VC155 plasmid Description

pBiFC-HA-HMGB2-VC155 This is a plasmid suitable for bimolecular fluorescence in mammalian cells.

pBiFC-HA-HMGB2-VC155 plasmid Sequence

1 ATGTACCCAT ACGATGTTCC AGATTACGCT CTTATGGCCA TGGAGGCCCG AATTCcaatg 61 ggtaaaggag accccaacaa gccgcggggc aaaatgtcct cgtacgcctt cttcgtgcag 121 acctgccggg aagagcacaa gaagaaacac ccggactctt ccgtcaattt cgcggaattc 181 tccaagaagt gttcggagag atggaagacc atgtctgcaa aggagaagtc gaagtttgaa 241 gatatggcaa aaagtgacaa agctcgctat gacagggaga tgaaaaatta cgttcctccc 301 aaaggtgata agaaggggaa gaaaaaggac cccaatgctc ctaaaaggcc accatctgcc 361 ttcttcctgt tttgctctga acatcgccca aagatcaaaa gtgaacaccc tggcctatcc 421 attggggata ctgcaaagaa attgggtgaa atgtggtctg agcagtcagc caaagataaa 481 caaccatatg aacagaaagc agctaagcta aaggagaaat atgaaaagga tattgctgca 541 tatcgtgcca agggcaaaag tgaagcagga aagaagggcc ctggcaggcc aacaggctca 601 aagaagaaga acgaaccaga agatgaggag gaggaggagg aagaagaaga tgaagatgag 661 gaggaagagg atgaagatga agaaggtgga agtggatcag gcagcggtgg atctGGTACC 721 CGTCCGGCGT GCAAAATCCC GAACGACCTG AAACAGAAAG TCATGAACCA CGACAAGCAG 781 AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGCCACAACA TCGAGGACGG CGGCGTGCAG 841 CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC 901 AACCACTACC TGAGCTACCA GTCCAAACTG AGCAAAGACC CCAACGAGAA GCGCGATCAC 961 ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC 1021 AAGTAAGCGG CCGCGGGGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA 1081 CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT 1141 TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT 1201 TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTCGGATCC TCTAGAGTCG ATCTGCAGGC 1261 ATGCTAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 1321 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 1381 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 1441 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 1501 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 1561 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 1621 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 1681 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 1741 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 1801 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 1861 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 1921 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 1981 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 2041 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 2101 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 2161 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 2221 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 2281 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 2341 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 2401 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 2461 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 2521 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 2581 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 2641 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 2701 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 2761 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 2821 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 2881 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 2941 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 3001 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 3061 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 3121 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 3181 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 3241 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 3301 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 3361 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 3421 GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA 3481 GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC 3541 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG 3601 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT 3661 CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA 3721 GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC 3781 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC 3841 GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG 3901 AGTTCGAGCT TGCATGCCTG CAGGTCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT 3961 GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC 4021 CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG 4081 CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT 4141 GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 4201 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 4261 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 4321 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 4381 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG 4441 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC 4501 GGGACCGATC CAGCCTCCGG ACTCTAGAGG ATCCGGTACT AGAGGAACTG AAAAACCAGA 4561 AAGTTAACTG GTAAGTTTAG TCTTTTTGTC TTTTATTTCA GGTCCCGGAT CCGGTGGTGG 4621 TGCAAATCAA AGAACTGCTC CTCAGTGGAT GTTGCCTTTA CTTCTAGGCC TGTACGGAAG 4681 TGTTACTTCT GCTCTAAAAG CTGCGGAATT GTACCCGCGG GCCCACC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.