pLV3-CMV-MCS-3×FLAG-Fluc-CopGF

pLV3-CMV-MCS-3×FLAG-Fluc-CopGFP-Neo plasmid

PVT91710

pLV3-CMV-MCS-3×FLAG-Fluc-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-MCS-3×FLAG-Fluc-CopGFP-Neo
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MCS-3×FLAG-Fluc-CopGFP-Neo plasmid Description

pLV3-CMV-MCS-3×FLAG-Fluc-CopGFP-Neo It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MCS-3×FLAG-Fluc-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagaGC 2281 TAGCGATATC GCCACCATGG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT 2341 CGACTACAAG GATGACGATG ACAAGgaatt cgccaccatg gaagacgcca aaaacataaa 2401 gaaaggcccg gcgccattct atccGctAga ggatggaacc gctggagagc aactgcataa 2461 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga 2521 ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt cggttggcag aagctatgaa 2581 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt 2641 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat 2701 ttataatgaa cgtgaattgc tcaacagtat gaacatttcg cagcctaccg tagtgtttgt 2761 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa 2821 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt 2881 cacatctcat ctacctcccg gttttaatga atacgatttt gtaccagagt cctttgatcg 2941 tgacaaaaca attgcactga taatgaactc ctctggatct actgggttac ctaagggtgt 3001 ggcccttccg catagaactg cctgcgtcag attctcgcat gccagagatc ctatttttgg 3061 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg 3121 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt 3181 tgaagaagag ctgtttttac gatcccttca ggattacaaa attcaaagtg cgttgctagt 3241 accaacccta ttttcattct tcgccaaaag cactctgatt gacaaatacg atttatctaa 3301 tttacacgaa attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg aagcggttgc 3361 aaaacgcttc catcttccag ggatacgaca aggatatggg ctcactgaga ctacatcagc 3421 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt 3481 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcagagagg 3541 cgaattatgt gtcagaggac ctatgattat gtccggttat gtaaacaatc cggaagcgac 3601 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga 3661 agacgaacac ttcttcatag ttgaccgctt gaagtcttta attaaataca aaggatacca 3721 ggtggccccc gctgaattgg agtcgatatt gttacaacac cccaacatct tcgacgcggg 3781 cgtggcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga 3841 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac 3901 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg 3961 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagtc 4021 caaattgtga ggatccgcgg ccgcaaggat ctgcgatcgc tccggtgccc gtcagtgggc 4081 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg 4141 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 4201 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 4261 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc 4321 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc 4381 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc 4441 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac 4501 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg 4561 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag 4621 cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt 4681 cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa 4741 gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg 4801 cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa 4861 caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt 4921 gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac 4981 cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt 5041 ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag 5101 cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc 5161 catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga 5221 gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat 5281 cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc 5341 cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga 5401 gaatcccggc cctATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT 5461 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT 5521 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC 5581 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC 5641 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA 5701 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT 5761 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA 5821 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA 5881 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC 5941 GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT 6001 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA 6061 CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG 6121 GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT 6181 CTATCGCCTT CTTGACGAGT TCTTCTGAaa tcaacctctg gattacaaaa tttgtgaaag 6241 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat 6301 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc 6361 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg 6421 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct 6481 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct 6541 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg 6601 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac 6661 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct 6721 gccggctctg cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct 6781 ttggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 6841 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata 6901 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 6961 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 7021 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 7081 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 7141 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 7201 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 7261 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg 7321 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 7381 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 7441 tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt 7501 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 7561 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 7621 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 7681 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 7741 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 7801 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 7861 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 7921 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 7981 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 8041 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 8101 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 8161 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 8221 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 8281 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 8341 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 8401 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 8461 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 8521 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 8581 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 8641 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 8701 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 8761 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 8821 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 8881 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 8941 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 9001 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 9061 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 9121 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 9181 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 9241 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 9301 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 9361 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 9421 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 9481 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 9541 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 9601 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 9661 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc 9721 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg 9781 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt 9841 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 9901 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc 9961 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 10021 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 10081 cgacgttgta aaacgacggc cagtgccaag ctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.