pLV3-EF1a-MCS-3×FLAG-PGK-Puro

pLV3-EF1a-MCS-3×FLAG-PGK-Puro plasmid

PVT90586

pLV3-EF1a-MCS-3×FLAG-PGK-Puro plasmid Information

Plasmid name: pLV3-EF1a-MCS-3×FLAG-PGK-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-MCS-3×FLAG-PGK-Puro plasmid Description

pLV3-EF1a-MCS-3×FLAG-PGK-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-MCS-3×FLAG-PGK-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cgtttaGaat cggatccGAC TACAAAGACC 2521 ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc 2581 cgctgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg 2641 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt 2701 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta 2761 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta 2821 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc 2881 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga 2941 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg 3001 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc 3061 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatggag agcgacgaga 3121 gcggcctgat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca 3181 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg 3241 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg 3301 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca 3361 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt 3421 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc 3481 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg 3541 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg 3601 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg 3661 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg 3721 gtgcctgagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 3781 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 3841 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 3901 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 3961 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 4021 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 4081 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 4141 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 4201 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 4261 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 4321 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 4381 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt 4441 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 4501 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 4561 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 4621 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 4681 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc 4741 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 4801 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc 4861 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 4921 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 4981 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt 5041 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 5101 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 5161 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 5221 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 5281 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 5341 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 5401 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 5461 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 5521 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 5581 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 5641 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 5701 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 5761 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 5821 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 5881 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 5941 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 6001 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 6061 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 6121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 6181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 6241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 6301 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 6361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 6421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 6481 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 6541 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 6601 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 6661 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 6721 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 6781 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 6841 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 6901 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 6961 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 7021 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 7081 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 7141 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 7201 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 7261 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 7321 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 7381 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 7441 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 7501 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 7561 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 7621 cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.