pCMV-ITCH-1-HA-Neo plasmid

pCMV-ITCH-1-HA-Neo plasmid

PVT91614

pCMV-ITCH-1-HA-Neo plasmid Information

Plasmid name: pCMV-ITCH(human)-1-HA-Neo
Alias: NM_001257137.3;ADMFD; AIF4; AIP4; NAPP1
Gene lenght: 2709bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ITCH-1-HA-Neo plasmid Description

pCMV-ITCH-1-HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-ITCH-1-HA-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGT 901 TAAGCTTGGT ACCGAGCTCG GATCCACTAG TCCAGTGTGG TGGAATTCGC CACCatgtct 961 gacagtggat cacaacttgg ttcaatgggt agcctcacca tgaaatcaca gcttcagatc 1021 actgtcatct cagcaaaact taaggaaaat aagaagaatt ggtttggacc aagtccttac 1081 gtagaggtca cagtagatgg acagtcaaag aagacagaaa aatgcaacaa cacaaacagt 1141 cccaagtgga agcaacccct tacagttatc gttacccctg tgagtaaatt acattttcgt 1201 gtgtggagtc accagacact gaaatctgat gttttgttgg gaactgctgc attagatatt 1261 tatgaaacat taaagtcaaa caatatgaaa cttgaagaag tagttgtgac tttgcagctt 1321 ggaggtgaca aagagccaac agagacaata ggagacttgt caatttgtct tgatgggcta 1381 cagttagagt ctgaagttgt taccaatggt gaaactacat gttcagaaaa tggggtttcg 1441 ctctgtttgc ccaggctgga gtgcaatagt gcgatctcgg ctcactgcaa cctctgcctc 1501 ccgggtttaa gtgattctcc tatctcagcc tcccgagtag ctgggtttac aggtgcttct 1561 cagaatgatg atggctccag atccaaggat gaaacaagag tgagcacaaa tggatcagat 1621 gaccctgaag atgcaggagc tggtgaaaat aggagagtca gtgggaataa ttctccatca 1681 ctctcaaatg gtggttttaa accttctaga cctccaagac cttcacgacc accaccaccc 1741 accccacgta gaccagcatc tgtcaatggt tcaccatctg ccacttctga aagtgatggg 1801 tctagtacag gctctctgcc gccgacaaat acaaatacaa atacatctga aggagcaaca 1861 tctggattaa taattcctct tactatatct ggaggctcag gccctaggcc attaaatcct 1921 gtaactcaag ctcccttgcc acctggttgg gagcagagag tggaccagca cgggcgagtt 1981 tactatgtag atcatgttga gaaaagaaca acatgggata gaccagaacc tctacctcct 2041 ggctgggaac ggcgggttga caacatggga cgtatttatt atgttgacca tttcacaaga 2101 acaacaacgt ggcagaggcc aacactggaa tccgtccgga actatgaaca atggcagcta 2161 cagcgtagtc agcttcaagg agcaatgcag cagtttaacc agagattcat ttatgggaat 2221 caagatttat ttgctacatc acaaagtaaa gaatttgatc ctcttggtcc attgccacct 2281 ggatgggaga agagaacaga cagcaatggc agagtatatt tcgtcaacca caacacacga 2341 attacacaat gggaagaccc cagaagtcaa ggtcaattaa atgaaaagcc cttacctgaa 2401 ggttgggaaa tgagattcac agtggatgga attccatatt ttgtggacca caatagaaga 2461 actaccacct atatagatcc ccgcacagga aaatctgccc tagacaatgg acctcagata 2521 gcctatgttc gggacttcaa agcaaaggtt cagtatttcc ggttctggtg tcagcaactg 2581 gccatgccac agcacataaa gattacagtg acaagaaaaa cattgtttga ggattccttt 2641 caacagataa tgagcttcag tccccaagat ctgcgaagac gtttgtgggt gatttttcca 2701 ggagaagaag gtttagatta tggaggtgta gcaagagaat ggttctttct tttgtcacat 2761 gaagtgttga acccaatgta ttgcctgttt gaatatgcag ggaaggataa ctactgcttg 2821 cagataaacc ccgcttctta catcaatcca gatcacctga aatattttcg ttttattggc 2881 agatttattg ccatggctct gttccatggg aaattcatag acacgggttt ttctttacca 2941 ttctataagc gtatcttgaa caaaccagtt ggactcaagg atttagaatc tattgatcca 3001 gaattttaca attctctcat ctgggttaag gaaaacaata ttgaggaatg tgatttggaa 3061 atgtacttct ccgttgacaa agaaattcta ggtgaaatta agagtcatga tctgaaacct 3121 aatggtggca atattcttgt aacagaagaa aataaagagg aatacatcag aatggtagct 3181 gagtggaggt tgtctcgagg tgttgaagaa cagacacaag ctttctttga aggctttaat 3241 gaaattcttc cccagcaata tttgcaatac tttgatgcaa aggaattaga ggtcctttta 3301 tgtggaatgc aagagattga tttgaatgac tggcaaagac atgccatcta ccgtcattat 3361 gcaaggacca gcaaacaaat catgtggttt tggcagtttg ttaaagaaat tgataatgag 3421 aagagaatga gacttctgca gtttgttact ggaacctgcc gattgccagt aggaggattt 3481 gctgatctca tggggagcaa tggaccacag aaattctgca ttgaaaaagt tgggaaagaa 3541 aattggctac ccagaagtca tacctgtttt aatcgcctgg acctgccacc atacaagagc 3601 tatgagcaac tgaaggaaaa gctgttgttt gccatagaag aaacagaagg atttggacaa 3661 gagCTCGAGT CTAGAGGGCC CTTCTACCCA TACGATGTTC CAGATTACGC TTGAGTTTAA 3721 ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC 3781 CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG 3841 GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG 3901 GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT 3961 ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT 4021 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 4081 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 4141 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 4201 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 4261 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 4321 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 4381 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA 4441 TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 4501 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC 4561 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC 4621 TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT 4681 GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA 4741 AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA 4801 TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG 4861 ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG 4921 CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC 4981 CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG 5041 CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA 5101 CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT 5161 CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA 5221 CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC 5281 GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC 5341 TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG 5401 TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG 5461 GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA 5521 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG 5581 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT 5641 GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA 5701 GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC 5761 GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC 5821 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT 5881 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT 5941 CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT 6001 CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG 6061 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG 6121 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG 6181 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC 6241 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC 6301 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG 6361 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT 6421 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG 6481 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA 6541 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG 6601 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT 6661 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC 6721 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC 6781 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT 6841 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC 6901 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC 6961 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 7021 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 7081 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG 7141 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT 7201 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA 7261 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA 7321 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 7381 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT 7441 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG 7501 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC 7561 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG 7621 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 7681 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA 7741 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA 7801 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 7861 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT 7921 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 7981 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 8041 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 8101 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.