pCMV-EGFP-CAPN3-Neo plasmid

pCMV-EGFP-CAPN3-Neo plasmid

PVT91569

pCMV-EGFP-CAPN3-Neo plasmid Information

Plasmid name: pCMV-EGFP-CAPN3(human)-Neo
Alias: NM_000070.3;CANP3; CANPL3; LGMD2; LGMD2A; LGMDD4;LGMDR1; nCL-1; p94
Gene lenght: 2466bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-CAPN3-Neo plasmid Description

pCMV-EGFP-CAPN3-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-CAPN3-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag gGaGGCGGAG GTAGTGGAGG AGGGGGAAGC 1621 ggaggcggag ggagtGAATT CGTCGACATG CCGACCGTCA TTAGCGCATC TGTGGCTCCA 1681 AGGACAGCGG CTGAGCCCCG GTCCCCAGGG CCAGTTCCTC ACCCGGCCCA GAGCAAGGCC 1741 ACTGAGGCTG GGGGTGGAAA CCCAAGTGGC ATCTATTCAG CCATCATCAG CCGCAATTTT 1801 CCTATTATCG GAGTGAAAGA GAAGACATTC GAGCAACTTC ACAAGAAATG TCTAGAAAAG 1861 AAAGTTCTTT ATGTGGACCC TGAGTTCCCA CCGGATGAGA CCTCTCTCTT TTATAGCCAG 1921 AAGTTCCCCA TCCAGTTCGT CTGGAAGAGA CCTCCGGAAA TTTGCGAGAA TCCCCGATTT 1981 ATCATTGATG GAGCCAACAG AACTGACATC TGTCAAGGAG AGCTAGGGGA CTGCTGGTTT 2041 CTCGCAGCCA TTGCCTGCCT GACCCTGAAC CAGCACCTTC TTTTCCGAGT CATACCCCAT 2101 GATCAAAGTT TCATCGAAAA CTACGCAGGG ATCTTCCACT TCCAGTTCTG GCGCTATGGA 2161 GAGTGGGTGG ACGTGGTTAT AGATGACTGC CTGCCAACGT ACAACAATCA ACTGGTTTTC 2221 ACCAAGTCCA ACCACCGCAA TGAGTTCTGG AGTGCTCTGC TGGAGAAGGC TTATGCTAAG 2281 CTCCATGGTT CCTACGAAGC TCTGAAAGGT GGGAACACCA CAGAGGCCAT GGAGGACTTC 2341 ACAGGAGGGG TGGCAGAGTT TTTTGAGATC AGGGATGCTC CTAGTGACAT GTACAAGATC 2401 ATGAAGAAAG CCATCGAGAG AGGCTCCCTC ATGGGCTGCT CCATTGATGA TGGCACGAAC 2461 ATGACCTATG GAACCTCTCC TTCTGGTCTG AACATGGGGG AGTTGATTGC ACGGATGGTA 2521 AGGAATATGG ATAACTCACT GCTCCAGGAC TCAGACCTCG ACCCCAGAGG CTCAGATGAA 2581 AGACCGACCC GGACAATCAT TCCGGTTCAG TATGAGACAA GAATGGCCTG CGGGCTGGTC 2641 AGAGGTCACG CCTACTCTGT CACGGGGCTG GATGAGGTCC CGTTCAAAGG TGAGAAAGTG 2701 AAGCTGGTGC GGCTGCGGAA TCCGTGGGGC CAGGTGGAGT GGAACGGTTC TTGGAGTGAT 2761 AGATGGAAGG ACTGGAGCTT TGTGGACAAA GATGAGAAGG CCCGTCTGCA GCACCAGGTC 2821 ACTGAGGATG GAGAGTTCTG GATGTCCTAT GAGGATTTCA TCTACCATTT CACAAAGTTG 2881 GAGATCTGCA ACCTCACGGC CGATGCTCTG CAGTCTGACA AGCTTCAGAC CTGGACAGTG 2941 TCTGTGAACG AGGGCCGCTG GGTACGGGGT TGCTCTGCCG GAGGCTGCCG CAACTTCCCA 3001 GATACTTTCT GGACCAACCC TCAGTACCGT CTGAAGCTCC TGGAGGAGGA CGATGACCCT 3061 GATGACTCGG AGGTGATTTG CAGCTTCCTG GTGGCCCTGA TGCAGAAGAA CCGGCGGAAG 3121 GACCGGAAGC TAGGGGCCAG TCTCTTCACC ATTGGCTTCG CCATCTACGA GGTTCCCAAA 3181 GAGATGCACG GGAACAAGCA GCACCTGCAG AAGGACTTCT TCCTGTACAA CGCCTCCAAG 3241 GCCAGGAGCA AAACCTACAT CAACATGCGG GAGGTGTCCC AGCGCTTCCG CCTGCCTCCC 3301 AGCGAGTACG TCATCGTGCC CTCCACCTAC GAGCCCCACC AGGAGGGGGA ATTCATCCTC 3361 CGGGTCTTCT CTGAAAAGAG GAACCTCTCT GAGGAAGTTG AAAATACCAT CTCCGTGGAT 3421 CGGCCAGTGA AAAAGAAAAA AACCAAGCCC ATCATCTTCG TTTCGGACAG AGCAAACAGC 3481 AACAAGGAGC TGGGTGTGGA CCAGGAGTCA GAGGAGGGCA AAGGCAAAAC AAGCCCTGAT 3541 AAGCAAAAGC AGTCCCCACA GCCACAGCCT GGCAGCTCTG ATCAGGAAAG TGAGGAACAG 3601 CAACAATTCC GGAACATTTT CAAGCAGATA GCAGGAGATG ACATGGAGAT CTGTGCAGAT 3661 GAGCTCAAGA AGGTCCTTAA CACAGTCGTG AACAAACACA AGGACCTGAA GACACACGGG 3721 TTCACACTGG AGTCCTGCCG TAGCATGATT GCGCTCATGG ATACAGATGG CTCTGGAAAG 3781 CTCAACCTGC AGGAGTTCCA CCACCTCTGG AACAAGATTA AGGCCTGGCA GAAAATTTTC 3841 AAACACTATG ACACAGACCA GTCCGGCACC ATCAACAGCT ACGAGATGCG AAATGCAGTC 3901 AACGACGCAG GATTCCACCT CAACAACCAG CTCTATGACA TCATTACCAT GCGGTACGCA 3961 GACAAACACA TGAACATCGA CTTTGACAGT TTCATCTGCT GCTTCGTTAG GCTGGAGGGC 4021 ATGTTCAGAG CTTTTCATGC ATTTGACAAG GATGGAGATG GTATCATCAA GCTCAACGTT 4081 CTGGAGTGGC TGCAGCTCAC CATGTATGCC TGAGCGGCCG CTCGAGTCTA GAGGGCCCGT 4141 TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC 4201 CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA 4261 TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG 4321 GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG 4381 CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC 4441 CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT 4501 TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC 4561 CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT 4621 ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC 4681 CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT 4741 GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT 4801 TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA 4861 TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 4921 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 4981 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 5041 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 5101 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC 5161 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT 5221 TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA 5281 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 5341 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 5401 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 5461 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 5521 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 5581 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 5641 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 5701 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 5761 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT 5821 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 5881 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG 5941 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 6001 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 6061 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 6121 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 6181 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 6241 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 6301 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 6361 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 6421 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 6481 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 6541 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 6601 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 6661 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 6721 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 6781 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 6841 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 6901 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 6961 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 7021 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 7081 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 7141 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 7201 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 7261 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 7321 CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 7381 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 7441 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 7501 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 7561 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 7621 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 7681 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 7741 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 7801 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 7861 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 7921 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 7981 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 8041 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 8101 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 8161 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 8221 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 8281 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 8341 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 8401 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 8461 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 8521 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC 8581 TCCCGATCCC CTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.