pCMV-CRY2(arabidopsis)(1-498aa

pCMV-CRY2(arabidopsis)(1-498aa)-CD2AP-EGFP-Neo plasmid

PVT91477

pCMV-CRY2(arabidopsis)(1-498aa)-CD2AP-EGFP-Neo plasmid Information

Plasmid name: pCMV-CRY2(arabidopsis)(1-498aa)-CD2AP(human)-EGFP-Neo
Alias: NM_012120.3;CMS;
Gene lenght: 1917bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): EPI300
Growth Temperature: 30°C
Vector backbone:
Forward primer:
Reverse primer: pEGFP-N-3'
Copy number: Low
Induction:

pCMV-CRY2(arabidopsis)(1-498aa)-CD2AP-EGFP-Neo plasmid Description

pCMV-CRY2(arabidopsis)(1-498aa)-CD2AP-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-CRY2(arabidopsis)(1-498aa)-CD2AP-EGFP-Neo plasmid Sequence

1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGGC CACCatgaag atggacaaaa agactatagt ttggtttaga 661 agagacctaa ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt 721 tttcctgtct tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca 781 agatggtgga tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct 841 gacctcactt taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt 901 accggtgcta caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac 961 cataccgtaa aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat 1021 ctattgtatg aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat 1081 tcttactgga agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg 1141 cggttgatgc caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta 1201 gggctggaga atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca 1261 ggatggagca atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat 1321 gcaaagaaca gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat 1381 ttcggggaaa taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg 1441 gcaagagata agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt 1501 ttaagagagt attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg 1561 ttgagtcatc ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga 1621 caaggcagga ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga 1681 tggatgcata acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt 1741 ccatggaaat ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt 1801 gacatccttg gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc 1861 ttggacaatc ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa 1921 tggcttcccg agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct 1981 ttaaccgtac tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta 2041 gacatcgaca cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag 2101 atcatgatcg gagcagcagg tggaggcgga tcaggaggcg gtggatctat ggttgactat 2161 attgtggagt atgactatga tgctgtacat gatgatgaat taactattcg agttggagaa 2221 atcatcagga atgtgaaaaa gctacaggag gaagggtggc tggaaggaga actaaatggg 2281 agaagaggaa tgttccctga caatttcgtt aaggaaatta aaagagagac ggaattcaag 2341 gatgacagtt tgcccatcaa acgggaaagg catgggaatg tagcaagtct tgtacaacga 2401 ataagcacct atggacttcc agctggagga attcagccac atccacaaac caaaaacatt 2461 aagaagaaga ccaagaagcg tcagtgtaaa gttctttttg agtacattcc acaaaatgag 2521 gatgaactgg agctgaaagt gggagatatt attgatatta atgaagaggt agaagaaggc 2581 tggtggagtg gaaccctgaa taacaagttg ggactgtttc cctcaaattt tgtgaaagaa 2641 ttagaggtaa cagatgatgg tgaaactcat gaagcccagg acgattcaga aactgttttg 2701 gctgggccta cttcacctat accttctctg ggaaatgtga gtgaaactgc atctggatca 2761 gttacacagc caaagaaaat tcgaggaatt ggatttggag acatttttaa agaaggctct 2821 gtgaaacttc ggacaagaac atccagtagt gaaacagaag agaaaaaacc agaaaagccc 2881 ttaatcctac agtcactggg acccaaaact cagagtgtgg agataacaaa aacagatacc 2941 gaaggtaaaa ttaaagctaa agaatattgt agaacattat ttgcctatga aggtactaat 3001 gaagatgaac ttacttttaa agagggggag ataatccatt tgataagtaa ggagactgga 3061 gaagctggct ggtggagggg cgaacttaat ggtaaagaag gagtatttcc agacaatttt 3121 gctgtccaga taaatgaact tgataaagac tttccaaaac caaagaaacc accacctcct 3181 gctaaggctc cagctccaaa gcctgaactg atagctgcag agaagaaata tttttcttta 3241 aagcctgaag aaaaggatga aaaatcaaca ctggaacaga aaccttctaa accagcagct 3301 ccacaagtcc cacccaagaa acctactcca cctaccaaag ccagtaattt actgagatct 3361 tctggaacag tgtacccaaa gcgacctgaa aaaccagttc ctccaccacc tcctatagcc 3421 aagattaatg gggaagtttc tagcatttca tcaaaatttg aaactgagcc agtatcaaaa 3481 ctaaagctag attctgaaca gctgcccctt agaccaaaat cagtagactt tgattcactt 3541 acagtaagga cctccaaaga aacagatgtt gtaaattttg atgacatagc ttcctcagaa 3601 aacttgcttc atctcactgc aaatagacca aagatgcctg gaagaaggtt gccgggccgt 3661 ttcaatggtg gacattctcc aactcacagc cccgaaaaaa tcttgaagtt accaaaagaa 3721 gaagacagtg ccaacctgaa gccatctgaa ttaaaaaaag atacatgcta ctctccaaag 3781 ccatctgtgt acctttcaac accttccagt gcttctaaag caaatacaac tgctttcctg 3841 actccattag aaatcaaagc taaagtggaa acagatgatg tgaaaaaaaa ttccctggat 3901 gaacttagag cccagattat tgaattgttg tgcattgtag aagcactgaa aaaggatcac 3961 gggaaagaac tggaaaaact gcgaaaagat ttggaagaag agaagacaat gagaagtaat 4021 ctagagatgg aaatagagaa gctgaaaaaa gctgtcctgt cttctGGCGG TGGAGGAAGC 4081 ggcggaggtg gatctatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 4141 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 4201 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 4261 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac 4321 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 4381 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 4441 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 4501 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 4561 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 4621 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 4681 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag 4741 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 4801 gagctgtaca agtagTAGCG GCCGCGACTC TAGATCATAA TCAGCCATAC CACATTTGTA 4861 GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG 4921 AATGCAATTG TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 4981 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC 5041 AAACTCATCA ATGTATCTTA AGGCGTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC 5101 GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC 5161 TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG 5221 TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA 5281 TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC 5341 ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA 5401 CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT 5461 AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC 5521 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT 5581 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG 5641 AAAAAGGAAG AGTCCTGAGG CGGAAAGAAC CAGCTGTGGA ATGTGTGTCA GTTAGGGTGT 5701 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 5761 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 5821 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 5881 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 5941 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 6001 GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA 6061 TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC 6121 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC 6181 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG ACGAGGCAGC 6241 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC 6301 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC 6361 TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC 6421 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG 6481 TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT 6541 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG AGGATCTCGT 6601 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG 6661 ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC 6721 CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG 6781 TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG 6841 AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT 6901 TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC 6961 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCTAGGGGG 7021 AGGCTAACTG AAACACGGAA GGAGACAATA CCGGAAGGAA CCCGCGCTAT GACGGCAATA 7081 AAAAGACAGA ATAAAACGCA CGGTGTTGGG TCGTTTGTTC ATAAACGCGG GGTTCGGTCC 7141 CAGGGCTGGC ACTCTGTCGA TACCCCACCG AGACCCCATT GGGGCCAATA CGCCCGCGTT 7201 TCTTCCTTTT CCCCACCCCA CCCCCCAAGT TCGGGTGAAG GCCCAGGGCT CGCAGCCAAC 7261 GTCGGGGCGG CAGGCCCTGC CATAGCCTCA GGTTACTCAT ATATACTTTA GATTGATTTA 7321 AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC 7381 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA 7441 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA 7501 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA 7561 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC 7621 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA 7681 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA 7741 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG 7801 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT 7861 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC 7921 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC 7981 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC 8041 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC 8101 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCATGCA T

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.