pLV2-TRE3GS-Usp16-TetOne-Puro

pLV2-TRE3GS-Usp16-TetOne-Puro plasmid

PVT91180

pLV2-TRE3GS-Usp16-TetOne-Puro plasmid Information

Plasmid name: pLV2-TRE3GS-Usp16(mouse)-TetOne-Puro
Alias: NM_024258.2;1200004E02Rik; 2810483I07Rik;6330514E22Rik; UBPM;
Gene lenght: 2478bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression,Tetracycline induction
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV2-TRE3GS-Usp16-TetOne-Puro plasmid Description

pLV2-TRE3GS-Usp16-TetOne-Puro is a plasmid suitable for protein expression in mammalian cells, tetracycline-inducible, and can also be used for lentivirus packaging.

pLV2-TRE3GS-Usp16-TetOne-Puro plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 4681 tactcggtca tggtcgcgtt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac 4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt 5041 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg 5101 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg 5161 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg 5221 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga 5281 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc 5341 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg 5401 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac 5461 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga 5521 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg 5581 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc 5641 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt 5701 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg 5761 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg 5821 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg 5881 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc 5941 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc 6001 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg 6061 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga 6121 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg 6181 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc 6241 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg 6301 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac 6361 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca 6421 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt 6481 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac 6541 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc 6601 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt 6661 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcGCCAC Catggggaag 6721 aaacggacca aggggagaag tgctccagac acggtggcct cagagtctgc agaaccagtg 6781 tgcagacacc ttagaaaagg gttggaacaa ggtaatttga aaaaagcttt agtaaatgtg 6841 gagtggaata tctgccaaga ctgtaagact gacaataaag tgaaagataa acctgaggag 6901 gaagcagaag acccttcggt ttggctctgt cttaaatgtg gccatcaggg ctgtggcaga 6961 gattctcagg agcagcatgc cttgaagcac tacacgacac cgagatccga gcctcactac 7021 ctggtgctca gtctggacaa ctggagcgtc tggtgctaca agtgtgacga ggaagtcaag 7081 tactgtagct caaaccgatt gggccaagtg gttgattatg ttagaaaaca agctggcgta 7141 agaacttcaa aaccagcaga gaaaaataat ggacacattg agctcgaaaa taaaaaattg 7201 gagaaagaga gtaaaaatga acaagagaga gagaaatcgg aaaacctggc taaagaaact 7261 attcccatgg actctgcttc ccagataact gtgaaaggac tcagtaattt ggggaatact 7321 tgtttcttca atgcagttat gcagaacttg tcacaaacgc cagtgcttag agaactacta 7381 aaagaagtga agatgtctgg aacgattgta aaaatagagc cacctgatct ggcactaaca 7441 gaacctttag aagtaaacct cgagcctcca ggtcctctta ctttagccat gagccagttt 7501 ctcagtgaga tgcaagagaa caaaaagcga gttgtgacac ctaaagagct cttttctcag 7561 gtctgtaaaa aagcaacacg ttttaaaggg taccagcaac aagacagcca ggagctgctt 7621 cgctacctac tggatgggat gagagcggaa gaacaccaaa gagtgagtaa aggaattctt 7681 aaagcatttg gtaattctac tgaaaaattg gatgaagaag taaaaaataa agttaaagat 7741 tatgaaaaga aaaaggcaat cccgagtttt gtggaccgca tctttggtgg cgagctgact 7801 agtacgatca tgtgtgatga atgcaggact gtctccttag tgcatgaatc gttccttgat 7861 ttgtctcttc cagttttaga tgatcagagt ggtaagaaaa gtataaatga taaaaatgtg 7921 aaaatgacaa tggaggaaga agataaagac agtgaggaag agaaagatga cagctacatg 7981 aaatcaagga gcgatcttcc gtcagggaca agcaagcacc tacagaaaaa ggcaaagaag 8041 caggccaaaa agcaggccaa gaaccaacga aggcaacaaa aaattcaaga aagatttctt 8101 cacttcaatg agctctgcgc cactgactac acggaagaca atgaacgtga agctgacaca 8161 gcacttgcgg gagaagtgga agtggatacc gactccaccc atggttctca agaggaggcc 8221 acacagatag agctgtctgt taaccagaag gatttggatg gccaagagag catgatagaa 8281 aggacacctg atgtgcagga aagcccagag gacctaggag tgaaaagtgc taacaccgag 8341 agtgatctgg ggattgtgac gcctgctcct gaatgtccta gggatttcaa tggtgccttc 8401 ctggaagaaa ggaccagtgg agaactagac attatcaatg gtttaaaaaa ccttaatttg 8461 aatgctgctg ttgatcctga tgaaataaat atagagattc cgaatgacag tcattctgca 8521 cccaaggtat atgaggtcat gaacgaggac ccagaaactg ctttctgtac cctcgcgaac 8581 cgagaagcgt ttagtactga tgagtgttcc attcaacatt gcttatatca gttcacccgg 8641 aatgagaaac ttcaagatgc caataaactg ctttgtgaag tgtgttcaag acggcagtgt 8701 aatggaccaa aggcaaatat aaaaggtgac aggagacatg tttacaccaa tgccaagaag 8761 cagatgctgg tctccctcgc gcctcctgtc ctcactctgc atttaaagcg attccagcag 8821 gctggtttta acctgcgcaa agttaacaaa cacataaagt ttccagaaat cttagatttg 8881 gctccttttt gtacccttaa atgtaagaat gttgctgaag aaagtacacg agtgctgtat 8941 tccttatatg gagttgttga acacagtggt actatgaggt cagggcatta cactgcctat 9001 gcgaaggaga gaactgcaag ctgtcacctc tccaatcttg ttcttcacgg tgacattcca 9061 caagattgtg aaatggaatc aaccaaaggg cagtggtttc acatcagcga tacacatgtg 9121 caagctgtgc ctataactaa agtactgaac tcacaagcgt atctcctatt ttatgagaga 9181 atactgtgag gatccagacc acctcccctg cgagctaagc tggacagcca atgacgggta 9241 agagagtgac atttttcact aacctaagac aggagggccg tcagagctac tgcctaatcc 9301 aaagacgggt aaaagtgata aaaatgtatc actccaacct aagacaggcg cagcttccga 9361 gggatttgag atccagacat gataagatac attgatgagt ttggacaaac caaaactaga 9421 atgcagtgaa aaaaatgcct tatttgtgaa atttgtgatg ctattgcctt atttgtaacc 9481 attataagct gcaataaaca agttaagtcg ataaactgga tctctgctgt ccctgtaata 9541 aacccgaaaa ttttgaattt ttgtaatttg tttttgtagt tctttagttt gtatgtctgt 9601 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt 9661 aaaggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc 9721 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct 9781 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa 9841 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc 9901 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt 9961 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt 10021 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag 10081 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc 10141 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg 10201 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag 10261 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg 10321 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca 10381 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg 10441 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat 10501 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt gctgtgcggt 10561 ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat 10621 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc 10681 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga 10741 tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat 10801 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga 10861 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc 10921 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc 10981 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc 11041 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc 11101 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt 11161 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg 11221 tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc 11281 tcgccactcc ccagtcccgc ccaggccacg cctccctgga aagtccccag cggaaagtcc 11341 cttgtagcaa gctcgatatc agcagttctt gaagtactcc ggatgcagct ctcgggccac 11401 gtgatgaaat gctaggcggc tgtcaaacct ccactctaac acttctctct ccgggtcatc 11461 catcccatgc aggctcacag ggtgtaacaa gctggtgttc tctcctttat tggcctcttc 11521 taccttatct ggctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct 11581 gacccctggc cctggtgtgt agttctgcta atcagggaag tagccttgtg tgtggtagat 11641 ccacagatca aggatatctt gtcttctttg ggagtgaatt agcccttcca

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.