pCMV-CHD7-3×HA-Neo plasmid

pCMV-CHD7-3×HA-Neo plasmid

PVT91248

pCMV-CHD7-3×HA-Neo plasmid Information

Plasmid name: pCMV-CHD7(human)-3×HA-Neo
Alias: NM_017780.4;CRG; HH5; IS3; KAL5
Gene lenght: 8991bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: WPRE-R
Copy number: High
Induction:

pCMV-CHD7-3×HA-Neo plasmid Description

pCMV-CHD7-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-CHD7-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatggc agatccagga 721 atgatgagtc tttttggcga ggatgggaat attttcagtg aaggtcttga aggcctcgga 781 gaatgtggtt acccggaaaa tccagtaaat cctatgggtc agcaaatgcc aatagaccaa 841 ggctttgcct ctttacagcc atcccttcat catccttcaa ctaatcaaaa tcaaacaaag 901 ctgacacatt ttgatcacta taatcagtat gaacaacaaa agatgcatct gatggatcag 961 ccgaacagaa tgatgagcaa cacccctggg aacggactcg cgtctccgca ctcgcagtat 1021 cacacccctc ccgttcctca ggtgccccat ggtggcagtg gtggcggtca gatgggtgtc 1081 taccctggca tgcagaatga gaggcatggg caatcctttg tggacagcag ctccatgtgg 1141 ggccccaggg ctgttcaggt accagaccag atacgagccc cctaccagca gcagcagcca 1201 cagccgcagc caccgcagcc ggctccgtcg gggccccctg cacagggcca ccctcagcac 1261 atgcagcaga tgggcagcta tatggcacgt ggggattttt ccatgcagca gcatggtcag 1321 ccacagcaga ggatgagcca gttttcccaa ggccaagagg gcctcaatca gggaaatcct 1381 tttattgcca cctcaggacc tggccacttg tcccacgtgc cccagcagag tcccagcatg 1441 gcaccttcct tgcgtcactc ggtgcagcag ttccatcacc acccctctac tgctctccat 1501 ggagaatccg ttgcccacag tcccagattc tccccgaatc ctccccaaca aggggctgtt 1561 aggccgcaaa cccttaactt tagttctcgg agccagacag tcccctctcc tactataaac 1621 aactcagggc agtattctcg atatccttac agtaacctaa atcagggatt agttaacaat 1681 acagggatga atcaaaattt aggccttaca aataatactc caatgaatca gtccgtacca 1741 agatacccca atgctgtagg attcccatca aacagtggtc aaggactaat gcaccagcag 1801 cccatccacc ccagtggctc acttaaccaa atgaacacac aaactatgca tccttcacag 1861 cctcagggaa cttatgcctc tccacctccc atgtcaccca tgaaagcaat gagtaatcca 1921 gcaggcactc ctcctccaca agtcaggccg ggaagtgctg ggataccaat ggaagttggc 1981 agttatccaa atatgcccca tcctcagcca tctcaccagc cccctggtgc catgggaatc 2041 ggacagagga atatgggccc cagaaacatg cagcagtctc gtccatttat aggcatgtcc 2101 tcggcaccaa gggaattgac tgggcacatg aggccaaatg gttgtcctgg tgttggcctt 2161 ggagacccac aagcaatcca ggaacgactg atacctggcc aacaacatcc tggtcaacag 2221 ccatcttttc agcagttgcc aacctgtcct ccactgcagc ctcacccggg cttgcaccac 2281 cagtcttcac ctccacaccc tcatcaccag ccttgggcac agctccaccc atcaccccag 2341 aacaccccgc agaaagtgcc tgtgcatcag cattccccgt cggagccctt tctagagaaa 2401 ccagtgccgg atatgactca ggttagtgga ccgaatgctc agctagtgaa gagtgatgat 2461 tacctgccat caatagaaca gcagccacaa caaaagaaga agaaaaagaa aaacaaccac 2521 attgtagcag aggatcccag taaaggtttt ggtaaagatg acttccctgg tggggtagat 2581 aaccaagaac taaataggaa ctcactggat gggtcccaag aagaaaaaaa gaaaaagaaa 2641 aggtcaaagg caaaaaaaga cccgaaggaa ccgaaagaac ccaaggagaa aaaagagccc 2701 aaggaaccca agaccccgaa agcccctaag attcccaaag agccaaagga aaagaaagca 2761 aaaactgcca cgccaaaacc caaatccagc aaaaagtcaa gtaataagaa acctgactca 2821 gaagcaagtg ctttgaagaa aaaggtcaac aagggaaaaa cagaaggttc tgaaaattca 2881 gacttagaca aaacaccccc accatctcct cctcctgaag aagatgagga cccaggtgtt 2941 cagaagagac ggtccagcag acaggtgaag agaaagcgct acactgaaga cctggagttc 3001 aagatttctg atgaggaggc agatgatgca gatgctgctg ggagggattc cccctccaac 3061 acctcccagt cagaacagca ggaatctgtt gatgcagaag gcccagtggt agaaaaaatt 3121 atgagcagtc gttcagtaaa aaagcagaag gaatctggag aggaggtaga aattgaggaa 3181 ttctatgtga aatacaaaaa cttctcttat cttcattgtc agtgggcatc tatagaagat 3241 ctggaaaaag ataagagaat tcagcaaaaa attaaacgat ttaaggcaaa gcagggccag 3301 aacaagttcc tttcagagat tgaggatgag ctttttaatc cagattatgt ggaggttgac 3361 cggataatgg actttgcacg tagcacagat gaccggggag agcctgtgac tcactatctg 3421 gtgaagtggt gttcacttcc ttatgaagac agcacgtggg agcggaggca ggacatagat 3481 caagcaaaga tcgaggagtt tgagaaacta atgtccaggg agccggaaac agagcgtgtg 3541 gagcgacctc ctgctgatga ttggaagaaa tcggagagtt ccagggagta taaaaacaat 3601 aacaaactca gggaatacca gttggaggga gtaaactggc tacttttcaa ttggtacaac 3661 atgcgaaact gcattttagc agatgaaatg ggtttgggaa aaactatcca gtccattaca 3721 tttctctatg agatatattt gaaaggaatc catggccctt ttttagtaat tgccccattg 3781 tccacaatcc ccaactggga aagggaattc cgaacctgga cagagttgaa cgtggttgtg 3841 tatcatggga gtcaagctag tcgtcggacc attcagttgt atgaaatgta cttcaaagat 3901 ccccagggtc gagtgataaa ggggtcctat aagtttcatg ccatcatcac tacatttgag 3961 atgattttga ctgattgtcc tgagctgcgg aatattccat ggcgctgtgt agtcattgat 4021 gaagcccaca ggctgaagaa caggaactgc aagctgttgg agggactcaa gatgatggac 4081 ttggaacaca aagtgctgct gacgggaacc ccactccaga acactgtgga agaactcttc 4141 agcttgcttc atttcttgga accaagtcgc ttcccttcag aaaccacatt tatgcaagaa 4201 tttggtgatc taaaaacaga agagcaggtg caaaaacttc aagctattct aaagccaatg 4261 atgttgagac gtctcaaaga ggatgtagaa aagaacttgg cccccaaaga agaaactatt 4321 attgaagttg agctaacaaa cattcagaag aaatattacc gagccatcct tgagaagaat 4381 ttcacatttc tttccaaagg cggtggtcaa gctaacgtac ctaacctatt aaacactatg 4441 atggaattgc ggaagtgctg caatcatccg taccttatca atggtgctga agagaaaatt 4501 ttggaagagt ttaaagaaac acacaatgca gagtctccag attttcagct ccaggcaatg 4561 atccaggctg ctggcaagct agtgctgatt gacaagctgc tgccaaaact gaaggctggt 4621 ggccacaggg tgcttatctt ttcccagatg gtgcgctgct tggacatact ggaagactac 4681 ctcattcaaa gacggtaccc atatgaaagg atcgacggcc gagtaagagg caacctccgc 4741 caggcagcta tcgacagatt ctccaaacct gattctgata ggtttgtttt cctcctgtgt 4801 acaagggcag gaggtttagg cattaacctc actgctgctg atacctgcat catctttgat 4861 tcagactgga atccccaaaa tgacctccag gctcaggcta gatgtcatag aataggacag 4921 agcaaatctg tgaaaatcta caggctgatt acaagaaatt cctatgaaag ggaaatgttc 4981 gacaaggcta gtttgaaact gggcctggat aaagctgtgc tacagtctat gagtggaaga 5041 gaaaatgcta ccaatggggt acaacagctt tccaagaaag aaatagagga tcttctacga 5101 aaaggggcct atggtgcact catggatgag gaggatgaag ggtctaaatt ctgtgaagaa 5161 gatattgatc agatcctcct acgtcgaacc cacaccatta ccattgagtc agaagggaaa 5221 ggttccacat ttgctaaggc cagttttgtt gcatctggaa ataggacaga tatttccttg 5281 gatgatccaa atttctggca aaagtgggct aagaaggctg aattggatat tgatgcctta 5341 aatgggagga acaacctggt tattgatact ccaagagtga gaaagcagac caggctctac 5401 agtgcagtga aggaagatga gctgatggag ttctcagact tggaaagtga ttctgaagaa 5461 aagccctgtg caaagccacg gcgtccccag gataagtcac agggctatgc aaggagtgaa 5521 tgtttcaggg tggagaagaa tctgcttgtc tatggttggg gacggtggac agacattctt 5581 tcccacggac gctataaacg ccaactcact gagcaagatg tagaaaccat ctgcagaacc 5641 atcctggtgt actgtcttaa tcattacaaa ggggatgaga atatcaaaag cttcatctgg 5701 gatctgatca cacccacagc ggatggccag actcgagcct tggtcaacca ttccggtttg 5761 tcagctcctg tgccaagggg aaggaaggga aagaaggtga aagcccagag cacacagccg 5821 gtggtgcagg atgccgactg gctggccagc tgcaacccag atgccctgtt ccaggaggac 5881 agctacaaga aacacctgaa gcatcactgt aacaaggtcc tgctgcgtgt ccgcatgctg 5941 tactacctaa gacaagaagt gataggagac caggcggata agatcttaga gggtgctgac 6001 tcaagtgaag ccgatgtgtg gatccctgaa cctttccatg ctgaagttcc tgcagattgg 6061 tgggataagg aagcagacaa atccctctta attggagtgt tcaaacatgg ctatgagaag 6121 tacaactcca tgcgagctga ccccgcgctg tgctttctgg aacgagtcgg tatgcctgat 6181 gccaaggcca tagctgccga gcaaagagga acagacatgc tagcagatgg tggtgacggg 6241 ggagaatttg atagagaaga tgaagaccca gaatataaac caaccagaac accgttcaaa 6301 gatgaaatag atgaatttgc aaattctcct tcagaggata aggaagaatc catggaaata 6361 catgccacag gcaagcacag tgagagtaat gctgagttag gccaacttta ctggcctaac 6421 acttcaaccc tgactacacg tctgcgccgg ctcattactg cctatcagcg cagctataaa 6481 aggcaacaga tgaggcaaga ggccctaatg aagactgacc ggcgcagacg gcggcctcga 6541 gaggaagtga gagctctgga agcggaaagg gaagctatta tatctgagaa gcggcaaaag 6601 tggacaagaa gagaagaggc tgatttttac cgtgtggtat ccacctttgg ggttattttt 6661 gaccctgtga aacagcaatt tgactggaac caatttagag cctttgccag gcttgacaaa 6721 aaatctgatg agagtttgga gaaatacttc agttgttttg tggccatgtg taggcgagta 6781 tgtcgaatgc ccgtcaagcc agatgatgaa ccgcccgacc tctcctccat aattgagccg 6841 atcacagagg agcgagcctc tcgaactctg taccgcattg agctgctacg gaagatccgc 6901 gagcaggttc tccatcaccc ccagctggga gagaggctta agctctgcca gccaagcttg 6961 gatctgccag agtggtggga gtgtggacgg catgaccgag acttgctggt tggtgctgct 7021 aaacacgggg tcagtcggac ggattatcac atcctcaatg accctgagtt atccttcttg 7081 gatgcacata aaaactttgc tcaaaacaga ggggcaggta atacatcttc cttgaaccca 7141 ctggcagttg gatttgtcca gactcctcca gtcatctcat ctgctcatat tcaagatgag 7201 agggtactgg aacaagccga aggcaaagtg gaggagcctg aaaacccagc tgccaaggag 7261 aaatgtgagg gcaaagaaga ggaagaagaa accgatggca gcgggaagga gagcaagcag 7321 gaatgtgagg cagaggccag ctctgtgaaa aatgaactga aaggtgttga ggtcggcgca 7381 gacactgggt ccaaatctat ttcagagaaa ggttccgaag aggatgaaga ggaaaagctg 7441 gaggatgacg ataagtcgga agagtcttcc cagcccgaag caggagctgt ctctagaggg 7501 aagaattttg atgaagaaag caatgcttcc atgagcactg ctagagatga aacccgagat 7561 ggattctaca tggaggacgg agatccttca gtagctcagc tccttcatga aagaacattt 7621 gccttctcgt tttggcctaa ggatagagta atgataaacc gcttagacaa catctgtgaa 7681 gcagtgttga aaggcaaatg gccagtaaat aggcgccaga tgtttgattt ccaaggcctc 7741 atcccaggtt acacacccac cacagtggac agccccttgc agaagaggag ctttgctgag 7801 ctctccatgg tcggccaagc cagcattagt gggagtgagg acatcactac gtctcctcag 7861 ttgtcaaagg aagatgccct caacctctct gtccctcgcc agcggaggag gaggaggaga 7921 aaaatcgaaa ttgaggccga aagagctgcc aagaggcgaa atctcatgga gatggttgcc 7981 cagcttcgag agtctcaggt ggtctcagaa aatggacaag aaaaagttgt agatttatca 8041 aaggcctcaa gagaggcaac aagctctacc tcaaattttt catctctttc ttcaaagttt 8101 atcttgccta atgtctcaac accagtgtct gatgccttta agactcaaat ggaactgctc 8161 caagcaggcc tttcgcgcac acccacaagg catctcctta atggctccct agtggatgga 8221 gagcctccca tgaagaggag gcggggaagg aggaaaaatg tggagggact tgatctgctt 8281 ttcatgagcc acaaacggac gtcattgagt gcagaggatg ctgaggtgac caaagctttt 8341 gaagaagata tagagacccc accaacaaga aacattcctt ctcccggaca gctggaccca 8401 gacacacgga tccctgttat caatcttgaa gatgggacta ggctggtggg ggaagatgct 8461 cctaaaaata aggatttagt tgaatggctg aagctgcacc ctacttacac tgttgatatg 8521 ccaagttatg taccaaagaa tgcagatgtg ctgttttcct catttcagaa accgaaacag 8581 aaacgacata gatgtcgaaa ccctaataaa ttggatataa acactttgac aggagaagaa 8641 agggtgcctg ttgtcaataa acgaaatggg aagaagatgg gtggagctat ggcgcctcca 8701 atgaaggatc tacccaggtg gctggaagaa aatcctgaat ttgcagttgc tccagactgg 8761 actgatatag ttaagcagtc tggttttgtt cctgagtcga tgtttgaccg ccttctcact 8821 gggcctgtag tgcggggaga gggagcgagc agaagaggaa gaaggcccaa aagtgagatc 8881 gccagagcag ccgcggccgc cgctgctgtg gcctccacgt cagggatcaa ccctttgctg 8941 gtgaacagcc tgtttgctgg aatggacctg acgagccttc agaatctcca gaatctccag 9001 tcgctccagc tggcaggcct catgggcttc cctccaggac tggcaacagc tgccaccgcc 9061 ggaggcgatg cgaagaaccc tgctgctgtg ctgcccctga tgctgccagg aatggcgggc 9121 ctgcccaacg tgtttggctt gggcgggctg ttgaataacc ctctgtcagc tgctactgga 9181 aacaccacta ctgcttctag tcaaggagaa ccggaagaca gcacttcaaa aggagaggag 9241 aaaggaaatg agaatgaaga cgagaacaaa gactctgaga aaagcacaga tgctgtttcg 9301 gctgctgact ctgcgaatgg atctgttggt gctgctactg ccccggctgg attgccctca 9361 aacccgctag ccttcaaccc tttcctcctg tccacaatgg ccccgggcct cttctaccca 9421 tccatgtttc tacctccagg actgggggga ttgacgctgc ctgggttccc agcattggca 9481 ggacttcaga atgccgtggg ctccagcgaa gaaaaggctg ctgacaaggc tgagggagga 9541 ccctttaaag atggagagac ccttgaaggc agcgatgccg aggagagcct ggataagact 9601 gcagagtcct ccctcttaga agacgaaata gcacagggtg aagagctaga ctcacttgat 9661 gggggggatg aaatagaaaa caatgaaaat gatgaaCTCG AGTACCCATA CGACGTACCT 9721 GATTATGCAA CTTATCCTTA TGATGTACCT GATTATGCTA GCTATCCTTA TGATGTCCCA 9781 GACTACGCAT AGTGAGGGCC CGATATCTCG ACAATCAACC TCTGGATTAC AAAATTTGTG 9841 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 9901 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA 9961 AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG 10021 TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC 10081 TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT 10141 GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT 10201 CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG 10261 GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC 10321 TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT 10381 CCCTTTGGGC CGCCTCCCCG CCTGGAAACG GGGGAGGCTA ACTGAAACAC GGAAGGAGAC 10441 AATACCGGAA GGAACCCGCG CTATGACGGC AATAAAAAGA CAGAATAAAA CGCACGGGTG 10501 TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC 10561 CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC 10621 CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG 10681 CAGATCTGCG CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA 10741 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 10801 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 10861 CTCTAAATCG GGGCATCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 10921 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 10981 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 11041 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGGGG ATTTCGGCCT 11101 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT 11161 GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 11221 GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG 11281 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT 11341 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT 11401 TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG 11461 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG 11521 ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC 11581 AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT 11641 CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT 11701 CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG 11761 GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG 11821 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC 11881 TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC 11941 TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA 12001 AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA 12061 ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG 12121 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG 12181 TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC 12241 TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC 12301 CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG 12361 GGGTTCGCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 12421 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 12481 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 12541 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 12601 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 12661 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 12721 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 12781 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 12841 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 12901 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 12961 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 13021 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 13081 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 13141 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 13201 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 13261 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG 13321 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 13381 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 13441 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 13501 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC 13561 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 13621 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 13681 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 13741 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 13801 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 13861 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 13921 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 13981 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 14041 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 14101 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 14161 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 14221 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 14281 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 14341 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 14401 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 14461 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 14521 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 14581 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 14641 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 14701 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 14761 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 14821 GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT 14881 ATGGTCGACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATCTGCTCCC 14941 TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA ATTTAAGCTA CAACAAGGCA 15001 AGGCTTGACC GACAATTGCA TGAAGAATCT GCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.