pLV3-CMV-PML-3×FLAG-CopGFP-Pur

pLV3-CMV-PML-3×FLAG-CopGFP-Puro plasmid

PVT91228

pLV3-CMV-PML-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-PML(human)-3×FLAG-CopGFP-Puro
Alias: NM_033238.3;MYL; PP8675; RNF71; TRIM19
Gene lenght: 2646bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-PML-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-PML-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-PML-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg agcctgcacc cgcccgatct 2341 ccgaggcccc agcaggaccc cgcccggccc caggagccca ccatgcctcc ccccgagacc 2401 ccctctgaag gccgccagcc cagccccagc cccagcccta cagagcgagc ccccgcttcg 2461 gaggaggagt tccagtttct gcgctgccag caatgccagg cggaagccaa gtgcccgaag 2521 ctgctgcctt gtctgcacac gctgtgctca ggatgcctgg aggcgtcggg catgcagtgc 2581 cccatctgcc aggcgccctg gcccctaggt gcagacacac ccgccctgga taacgtcttt 2641 ttcgagagtc tgcagcggcg cctgtcggtg taccggcaga ttgtggatgc gcaggctgtg 2701 tgcacccgct gcaaagagtc ggccgacttc tggtgctttg agtgcgagca gctcctctgc 2761 gccaagtgct tcgaggcaca ccagtggttc ctcaagcacg aggcccggcc cctagcagag 2821 ctgcgcaacc agtcggtgcg tgagttcctg gacggcaccc gcaagaccaa caacatcttc 2881 tgctccaacc ccaaccaccg cacccctacg ctgaccagca tctactgccg aggatgttcc 2941 aagccgctgt gctgctcgtg cgcgctcctt gacagcagcc acagtgagct caagtgcgac 3001 atcagcgcag agatccagca gcgacaggag gagctggacg ccatgacgca ggcgctgcag 3061 gagcaggata gtgcctttgg cgcggttcac gcgcagatgc acgcggccgt cggccagctg 3121 ggccgcgcgc gtgccgagac cgaggagctg atccgcgagc gcgtgcgcca ggtggtagct 3181 cacgtgcggg ctcaggagcg cgagctgctg gaggctgtgg acgcgcggta ccagcgcgac 3241 tacgaggaga tggccagtcg gctgggccgc ctggatgctg tgctgcagcg catccgcacg 3301 ggcagcgcgc tggtgcagag gatgaagtgc tacgcctcgg accaggaggt gctggacatg 3361 cacggtttcc tgcgccaggc gctctgccgc ctgcgccagg aggagcccca gagcctgcaa 3421 gctgccgtgc gcaccgatgg cttcgacgag ttcaaggtgc gcctgcagga cctcagctct 3481 tgcatcaccc aggggaaaga tgcagctgta tccaagaaag ccagcccaga ggctgccagc 3541 actcccaggg accctattga cgttgacctg cccgaggagg cagagagagt gaaggcccag 3601 gttcaggccc tggggctggc tgaagcccag cctatggctg tggtacagtc agtgcccggg 3661 gcacaccccg tgccagtgta cgccttctcc atcaaaggcc cttcctatgg agaggatgtc 3721 tccaatacaa cgacagccca gaagaggaag tgcagccaga cccagtgccc caggaaggtc 3781 atcaagatgg agtctgagga ggggaaggag gcaaggttgg ctcggagctc cccggagcag 3841 cccaggccca gcacctccaa ggcagtctca ccaccccacc tggatggacc gcctagcccc 3901 aggagccccg tcataggaag tgaggtcttc ctgcccaaca gcaaccacgt ggccagtggc 3961 gccggggagg cagaggaacg cgttgtggtg atcagcagct cggaagactc agatgccgaa 4021 aactcgtcct cccgagagct ggatgacagc agcagtgagt ccagtgacct ccagctggaa 4081 ggccccagca ccctcagggt cctggacgag aaccttgctg acccccaagc agaagacaga 4141 cctctggttt tctttgacct caagattgac aatgaaaccc agaagattag ccagctggct 4201 gcggtgaacc gggaaagcaa gttccgcgtg gtcatccagc ctgaagcctt cttcagcatc 4261 tactccaagg ccgtgtccct ggaggtgggg ctgcagcact tcctcagctt tctgagctcc 4321 atgcgccgcc ctatcttggc ctgctacaag ctgtgggggc ctggcctccc aaacttcttc 4381 cgggccctgg aggacattaa caggctgtgg gaattccagg aggccatctc gggcttcctg 4441 gctgccctgc ctctcatccg ggagcgtgtg cccggggcca gcagcttcaa actcaagaac 4501 ctggcccaga cctacctggc gagaaacatg agcgagcgca gcgccatggc tgccgtgctg 4561 gccatgcgtg acctgtgccg cctcctcgag gtctccccgg gcccccagct ggcccagcat 4621 gtctacccct tcagtagcct gcagtgcttt gcctccctgc agcccctggt gcaggcagct 4681 gtgctgcccc gggctgaggc ccgcctcctg gccctacaca acgtgagctt catggagctg 4741 ctgagtgcac accgccgtga ccggcagggg ggcctgaaga agtacagccg ctatctaagc 4801 ctgcagacca ccacgttgcc ccctgcccag cctgctttca acctgcaggc tctgggcacc 4861 tactttgaag gcctgttgga gggtccggcg ctggcacggg cagaaggagt ctccacccca 4921 cttgctggcc gtggcttggc agagagggcc tcccagcaga gcggatccGA CTACAAAGAC 4981 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGtagcgg 5041 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 5101 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 5161 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 5221 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 5281 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 5341 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 5401 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 5461 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 5521 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 5581 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 5641 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 5701 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 5761 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 5821 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 5881 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 5941 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 6001 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 6061 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 6121 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 6181 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 6241 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 6301 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 6361 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 6421 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 6481 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 6541 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 6601 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 6661 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 6721 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 6781 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 6841 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 6901 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 6961 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 7021 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 7081 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 7141 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 7201 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 7261 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 7321 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 7381 actgacaatt ccgtggtgtt gtcggggaaA TCATCGTCCT TTCCTtggct gctcgcctgt 7441 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 7501 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctTcgcctt 7561 cgccctcaga cgagtcggat ctccctttgg Gccgcctccc cgcctggtac ctttaagacc 7621 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 7681 agggctaatt cactcccaac gaaGataaga tctgcttttt gcttgtactg ggtctctctg 7741 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 7801 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 7861 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 7921 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 7981 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 8041 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 8101 ttatcatgtc tggctctagc taTCCCGCCC CTAACTCCGC CCAtcccgcc cctaactccg 8161 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 8221 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 8281 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 8341 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 8401 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 8461 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 8521 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 8581 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 8641 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 8701 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 8761 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 8821 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 8881 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 8941 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 9001 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 9061 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 9121 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 9181 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 9241 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 9301 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 9361 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 9421 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 9481 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 9541 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 9601 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 9661 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 9721 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 9781 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 9841 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 9901 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 9961 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 10021 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 10081 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 10141 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 10201 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 10261 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 10321 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 10381 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 10441 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 10501 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 10561 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 10621 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 10681 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 10741 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 10801 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 10861 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 10921 ggccagtgcc aagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.