pAAV-F4-80-Zbp1-3×HA-T2A-EGFP

pAAV-F4-80-Zbp1-3×HA-T2A-EGFP plasmid

PVT96823

pAAV-F4-80-Zbp1-3×HA-T2A-EGFP plasmid Information

Plasmid name: pAAV-F4-80(mouse)-Zbp1(mouse)-3×HA-T2A-EGFP
Alias: NM_021394.2;2010010H03Rik; Dai; Dlm1; mZaDLM;
Gene lenght: 1233bp
Host: Mammalian cells,Adeno associated virus
Use(s):
Promoter detection
Fragment type: Promoter
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: F4-80
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: Forward left ITR
Reverse primer:
Copy number: High
Induction:

pAAV-F4-80-Zbp1-3×HA-T2A-EGFP plasmid Description

pAAV-F4-80-Zbp1-3×HA-T2A-EGFP is a plasmid suitable for promoter detection in mammalian cells, and can also be used for adeno-associated virus packaging.

pAAV-F4-80-Zbp1-3×HA-T2A-EGFP plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtGTGTC TAGAacgcgt gctagcgcgg 181 ccgcgaattc tttgtttagg tctgtatgcc atatttaata gggttatttg gttctctgga 241 gtctaacttc ctgagttctt tgtatatttt ggatatcagt cctctatcag atgttgggtt 301 ggtaaagatt tttttccccc aatctgtgag ttgctgtttt gtcatattga cagtctcctt 361 tgctttacag aagcttttta atttcatgag gtcccatttg tcaattgttg atcttagagc 421 ctgagccatt ggtgttctgt acaggacaaa ttttcccctg tgccaatatg ttcgaggttc 481 ttccccattt tcttttctat tagactcagt gtatctggtt ttatgtggag gtccttgatc 541 cactcggact tgagctttgt acaaggagat aagaatggat cagtttgtat ttttctacat 601 cctaactgcc agttgaacta gcaacatttg ttgaaaatgt ttttttttct tccctcactg 661 gatgactttg acttctttgt caaagatcaa gtgaccatag gtgtgtaggt tcacttttgg 721 gtcttcaatt ttattccatt gatctacctt tctgtcattg tatcaatacc atgaaggttt 781 tttaaaaaaa aattttttta atcatattgc tctgcagtac agcttgaggt cagggatggt 841 gattccccca gaggctcttt gattgttgag aacggttttt gctatcctgg gttttttgtt 901 attccagatg aatttgagtt gggattttga tagggaatac actgaatttg tagattgctt 961 ttggcaagat ggccatcttt ccatcttctg aggtctttga tttctttctt cagagacttg 1021 aagttcttgt catacagatc tttcaattgc ttggttagag tcacaccaag atattttata 1081 ttatttgtga ctattgtgaa gggtgtcatt tctctaattt atttctcagc ccgtttatcc 1141 tttgagtaga ggaaaactac tgatttgttt gaattaattt tatatctagc cactaagctg 1201 aagttgttta tcagctgtag gagttctctg gtggattatt ttttagggtt atttatgtat 1261 tctaccacat catctgcaaa tagtgatatc ttgacttcct cctttctaat ttgtatccct 1321 ttgacctcct tttgttgcct aattgttctg ggtagaactt tgagtactat attaaatgat 1381 aggggaaaag tggacagcct tgggtgtggt gtgtgtggta tggtgtggtg tgtgtatggt 1441 gatgatgcat gtggtgtgta tggtgtggtg tatgtggtgt ggtgtgtggt ggtggtgata 1501 atgatgatgg tggtctctct gtctctgtct gtctctttct atctctgcct ctctctctct 1561 gtctctctct gcccccctct gtctccctct gtctgtctgt ctgtctcttt ggtgtgatgt 1621 atgtggtgtg tgtgtattct acaaggttga catgatgaca gaatttaatt ttcttagcag 1681 caagctcatg gatcctggtg ataaatgcag catgacttta ctgaaaaggc tttgtgatct 1741 tgaagagtgg attgacttca ctgtcggcag cacatgcaat ctcacttgtt tggtgtaatg 1801 aaagaagaga atgagaggtg gaagggggat ggtaatgttg aaaaaaagaa tggtacagag 1861 gaaactgagg ttggagagag atggggtaga tggtaagaga tggagaaaga gggaaggaaa 1921 tggagagaaa gacagagaga cagagagaga cacacagaga gacacacaga gacagagagg 1981 aagggaaagg gaaagagaaa ggaagaggaa gagggggagg ggaaggggaa ggggaagggg 2041 aagggagagg gagaaatgtg gacactagcc agatttaagg gagaaattag ggggttgcca 2101 gtctgtccac ctctgatggt ggcaactcag cagaaagctg ctgggctcag tctggctttg 2161 ttgagcaacc ctgactccac cccttttctt ccccacaaag caagctttta aagggaaggc 2221 tttcttcatt gaatgactgc cacagtacga tgggtaccgt cgacgccacc atggcagaag 2281 ctcctgttga cttgagcaca ggagacaatc tggagcaaaa gatcctgcag gtgttgagcg 2341 atgacggcgg ccctgtgaag attggccagc tggtgaagaa atgccaagtg cccaagaaaa 2401 ccctcaatca agtcctttac cgcctgaaga aggaggacag agtgtcctcc ccagagcctg 2461 caacatggag cataggcggg gctgcttctg gagatggggc tcctgcaatc cctgagaact 2521 ccagtgccca gcctagcctt gatgaaagaa tattaagatt cctggaagcc aatgggcctc 2581 acagggccct gcacatcgcc aaggctctgg gaatgacgac agccaaagaa gtgaacccac 2641 tcctgtattc catgagaaat aagcaccttc tgagctatga cggacagacg tggaagatct 2701 accactcacg tcaggaaggc caagacatag ctcattctgg agtcacacaa gagtcccctg 2761 cgattatttg tcagcacaat ccggtcaaca tgatctgcca acaaggagcc aacagccaca 2821 tctccattgc caattcaaac gccatccaga ttggtcacgg gaatgtcata gtaagagaga 2881 aagcctgtgg tgagccaggt cccaggacct ctcaccctct acccttggca tgggacgcct 2941 ctgctcagga catgccccct gttgcccacg gagctcagta catctacatg gacaagtcct 3001 tgctccaaca agtgcagctt ggccaccaca acgagatgag cctcgtgggg gatgcaggga 3061 agcacccctc ttatagcttt tctgacagcc ccccagaagt gtcaaccacc actgctgacc 3121 caggagcttc attcaacatg caaacatctg agccaggccc tcaccctgag ggagacacag 3181 tccagacagt ccacatcaaa tcctgctttc tagaggacgc caccattggt aacggcaaca 3241 agatgaccat ccaccttagg tcaaagggtg aagtcatgga gtctggagac agtgaagagc 3301 cgaagaagga agacacaggt acaagctctg aagccactcc acccagaagc tgccagcaca 3361 cgcccagtga ctccatgctg cccacctctg agctgagagc tatggctctg ggagacagta 3421 gcccccagac cacagaacct gtgcttagag agcatgaagt ccaggacata gaaagctctc 3481 aagacacagg actgagcaag caaTACCCAT ACGACGTACC TGATTATGCA ACTTATCCTT 3541 ATGATGTACC TGATTATGCT AGCTATCCTT ATGATGTCCC AGACTACGCA ACTAGTGAGG 3601 GCAGGGGAAG TCTTCTAACA TGCGGGGACG TGGAGGAAAA TCCCGGCCCC gccaccatgg 3661 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 3721 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 3781 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 3841 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 3901 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 3961 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 4021 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 4081 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 4141 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 4201 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 4261 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 4321 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaAAGC 4381 TTTAAACCGG TTATCGATAT CGATACCGAG CGCTGCTCGA GAGATCTACc tgattttgta 4441 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc 4501 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc 4561 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg 4621 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat 4681 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 4741 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 4801 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 4861 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg 4921 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 4981 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt 5041 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 5101 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa 5161 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 5221 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 5281 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 5341 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 5401 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 5461 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 5521 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 5581 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 5641 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 5701 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 5761 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 5821 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 5881 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 5941 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 6001 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 6061 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 6121 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 6181 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 6241 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 6301 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 6361 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 6421 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 6481 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 6541 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 6601 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 6661 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 6721 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 6781 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 6841 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 6901 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 6961 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 7021 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 7081 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 7141 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 7201 acatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.