pLV3-CMV-CLIC5-3×FLAG-CopGFP-P

pLV3-CMV-CLIC5-3×FLAG-CopGFP-Puro plasmid

PVT91220

pLV3-CMV-CLIC5-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CLIC5(human)-3×FLAG-CopGFP-Puro
Alias: NM_001114086;MST130; MSTP130
Gene lenght: 1230bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CLIC5-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-CLIC5-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CLIC5-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGA ATGACGAAGA CTACAGCACC 2341 ATCTATGACA CAATCCAAAA TGAGAGGACG TATGAGGTTC CAGACCAGCC AGAAGAAAAT 2401 GAAAGTCCCC ATTATGATGA TGTCCATGAG TACTTAAGGC CAGAAAATGA TTTATATGCC 2461 ACTCAGCTGA ATACCCATGA GTATGATTTT GTGTCAGTCT ATACCATTAA GGGTGAAGAG 2521 ACCAGCTTGG CCTCTGTCCA GTCAGAAGAC AGAGGCTACC TCCTGCCTGA TGAGATATAC 2581 TCTGAACTCC AGGAGGCTCA TCCAGGTGAG CCCCAGGAGG ACAGGGGCAT CTCAATGGAA 2641 GGGTTATATT CATCAACCCA GGACCAGCAA CTCTGCGCAG CAGAACTCCA GGAGAATGGG 2701 AGTGTGATGA AGGAAGATCT GCCTTCTCCT TCAAGCTTCA CCATTCAGCA CAGTAAGGCC 2761 TTCTCTACCA CCAAGTATTC CTGCTATTCT GATGCTGAAG GTTTGGAAGA AAAGGAGGGA 2821 GCTCACATGA ACCCTGAGAT TTACCTCTTT GTGAAGGCTG GAATCGATGG AGAAAGCATC 2881 GGCAACTGTC CTTTCTCTCA GCGCCTCTTC ATGATCCTCT GGCTGAAAGG AGTCGTGTTC 2941 AATGTCACCA CTGTGGATCT GAAAAGAAAG CCAGCTGACC TGCACAACCT AGCCCCCGGC 3001 ACGCACCCGC CCTTCCTGAC CTTCAACGGG GACGTGAAGA CAGACGTCAA TAAGATCGAG 3061 GAGTTCCTGG AGGAGACCTT GACCCCTGAA AAGTACCCCA AACTGGCTGC AAAACACCGG 3121 GAATCCAACA CAGCGGGCAT CGACATCTTT TCCAAGTTTT CTGCCTACAT CAAAAATACC 3181 AAGCAGCAGA ACAATGCTGC TCTTGAAAGA GGCCTAACCA AGGCTCTAAA GAAATTGGAT 3241 GACTACCTGA ACACCCCTCT ACCAGAGGAG ATTGACGCCA ACACTTGTGG GGAAGACAAG 3301 GGGTCCCGGC GCAAGTTCCT GGATGGGGAT GAGCTGACCC TGGCTGACTG CAATCTGTTG 3361 CCCAAGCTCC ATGTGGTCAA GATTGTGGCC AAGAAATACC GCAACTATGA TATCCCGGCT 3421 GAGATGACAG GCCTGTGGCG GTACCTCAAG AACGCCTATG CCCGTGATGA GTTCACCAAC 3481 ACCTGTGCAG CTGACAGTGA GATCGAGTTG GCCTACGCTG ATGTCGCCAA ACGCCTCAGC 3541 CGATCCggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 3601 AAGGATGACG ATGACAAGta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 3661 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 3721 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 3781 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 3841 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 3901 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 3961 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 4021 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 4081 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 4141 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 4201 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 4261 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 4321 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 4381 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 4441 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 4501 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 4561 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 4621 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 4681 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 4741 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 4801 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 4861 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 4921 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 4981 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 5041 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 5101 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 5161 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 5221 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5281 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5341 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5401 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5461 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5521 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 5581 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 5641 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 5701 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 5761 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 5821 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 5881 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 5941 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaATCATCG 6001 TCCTTTCCTt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 6061 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 6121 cggcctcttc cgcgtctTcg ccttcgccct cagacgagtc ggatctccct ttggGccgcc 6181 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 6241 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat aagatctgct 6301 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6361 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6421 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6481 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6541 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6601 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6661 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctaTCCC GCCCCTAACT 6721 CCGCCCAtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 6781 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 6841 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata 6901 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6961 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 7021 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 7081 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 7141 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7201 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7261 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7321 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7381 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7441 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7501 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7561 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7621 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7681 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 7741 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7801 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7861 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7921 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7981 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 8041 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 8101 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 8161 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 8221 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8281 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8341 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8401 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8461 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8521 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8581 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8641 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8701 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 8761 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8821 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8881 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8941 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 9001 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 9061 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 9121 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 9181 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 9241 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 9301 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 9361 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 9421 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 9481 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.