pShox2-Fluc plasmid

pShox2-Fluc plasmid

PVT96457

pShox2-Fluc plasmid Information

Plasmid name: pShox2(mouse)-Fluc
Alias: -
Gene lenght: 2215bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: Shox2
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: RVP3
Reverse primer:
Copy number: High
Induction:

pShox2-Fluc plasmid Description

pShox2-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pShox2-Fluc plasmid Sequence

1 ggtaccgaag gataatgtca aagaaaaagt aaagcaacat ttctaataag ctttattttc 61 atgtccttta aatgtgcaat gtagttagta ttacaagaag ttatggaacg tcttaaatta 121 cacttaaatt ctcgactgag aatacattat gtaccacaat tctgtgttct ctttaccaat 181 gttttaggtt gtttgggtag aacttctgaa attattggct aagtgtgttt tgttgttcta 241 atcaataagt agaagatata catattaatg gcctgtaact gctttcattt ttactgagta 301 ctggatccaa gagaacgaaa acattatctt ttaacatcaa acaaaactca ttaatattca 361 gtatactgaa aatgcccccc caaactgcca cttaaagagg tatatttgtt tacatattca 421 agtaaatgta acatttcata tgtaaacatt cttttgtgtg agcaaataag tatttgcata 481 gctacaaatc acacagcaag ccgtggctta gaggagacag tcatgtcccc cccccccata 541 taattaagag tttttataaa ataattgtca aatatacagt ttttaagtta taaatcagaa 601 agagagttgt tttcccccct ccaaagagtg agtctttttt tcacagtcca cggtaaacaa 661 aaattagtcg agcgacaagc aacatcggta attttttccg cagaactcaa aaagtagtca 721 tcttttgttt ttcagaatta ggaaatcgaa ctagaatgcc ccccaagatg ctaaataatt 781 aagggcaggg tgcagggagt tgttaggaaa aggagtaaga acccttagac ctcatagaga 841 aaatcgaaat ggaactgcgc ctgaccgggc tgtaagaaaa taccttaaga ccgaatctgg 901 cagagaacag ctgctcagga aacaagttaa atcgaccaac cccttctagc gggatgtgtc 961 tttatgggag tcagctttga ctccaaagta gtagagtgaa ttacagggac cagtactcag 1021 tgaggctggc tgcgcgttcg gaagatccta aaaggaggtg aagccttccg aggtgctgca 1081 atgaagcccc attcgcagca ggcgcgtgaa gaccggatgc accgctgtgc agctgcagcg 1141 gcttggagca agaaagggtg caggaacgga atactggaat ggttgctagg cctattaacc 1201 atagagagcc cgatactggg ccagagacca ggggtcatca ttgtagggtc gagcagttgg 1261 gatgcccacc cagagcgagg ctgtagggtc gggtactctc tgaggatacc cactgactct 1321 gattgcaatg cacttgtcag tttcctcttg gccccgcctc cgagaagtct atccagttcc 1381 ccagtgtttt actaagtcgt gacaccaacg caaaatccac gcttaattaa attaattagg 1441 gattcttgtc aatcctggat taatggccaa gagcaccagc ggccgaggct atcagaaaac 1501 ccgaccgctg aggacctgcc tcctctcccc gccccctctg gtcccctgct tgccctccct 1561 ctccagcccg gtggactcca gcctggatcc tccatcctca atattcatat tcatattctc 1621 tctctctctc tctctctctc tctctctctc tctctctctc tctctctctc tctctctctc 1681 tctcgtcccc actccctctc ctccccaccc caggtagtgg cgctcctgct acccgcgaag 1741 gggttcacta ggagttgcga ggggaggtaa tttactcaga acaagaggcc cgcctttagg 1801 aagtataaat agtgagactt caagagctgc gtcgctatca gatctgaact ctccgcagga 1861 agaattgtca aagatcttaa cattgaacac gcgcactccg gcagggaagc atcagttccc 1921 atttccctct ggcggccccc gccccttccc tcctcccccc tctccccctt taccccctcc 1981 ttcctcctcc tcctcctcgt cctcctcctc ctcttacttc tttttctcct tctacttctc 2041 ctcttctttc ttctcctctt tttcttcttc ctcctcctcc ctctcctccc ccatccccct 2101 gccccattga tgtgttatta ttgggggggc tggagcagta aaaaaagaag gaggaaaaaa 2161 agagcggggc tcggcaggga gagcttgagc gcgaggttga ccggcggcgg cagcggccgc 2221 gaagcttggc attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag 2281 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag 2341 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag 2401 gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa 2461 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc 2521 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt 2581 tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt 2641 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa 2701 attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc 2761 acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg 2821 gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc 2881 gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc 2941 aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga 3001 atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt 3061 gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg 3121 ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat 3181 ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc 3241 aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct 3301 attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt 3361 tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc 3421 gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc 3481 aacgccttga ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa 3541 gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag 3601 gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt 3661 gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag 3721 cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc 3781 gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga 3841 aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc 3901 gccgtgtaat tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt 3961 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt 4021 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac 4081 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag 4141 taaaacctct acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc 4201 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat 4261 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct 4321 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 4381 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 4441 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 4501 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 4561 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 4621 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 4681 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 4741 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 4801 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 4861 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 4921 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 4981 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 5041 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 5101 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 5161 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 5221 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 5281 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 5341 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 5401 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 5461 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 5521 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 5581 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 5641 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 5701 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 5761 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 5821 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 5881 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 5941 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 6001 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 6061 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 6121 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 6181 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 6241 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 6301 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 6361 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 6421 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 6481 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 6541 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 6601 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 6661 acgcgaattt taacaaaata ttaacgttta caatttccca ttcgccattc aggctgcgca 6721 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat 6781 gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt 6841 attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct 6901 ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc 6961 aggtgccaga acatttctct atcgata

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.