pLV3-U6-PASD-shRNA1-Fluc-Hyg p

pLV3-U6-PASD-shRNA1-Fluc-Hyg plasmid

PVT91174

pLV3-U6-PASD-shRNA1-Fluc-Hyg plasmid Information

Plasmid name: pLV3-U6-PASD(human)-shRNA1-Fluc-Hyg
Alias: CAAGTGACGCTACAGTTATTA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-PASD-shRNA1-Fluc-Hyg plasmid Description

pLV3-U6-PASD-shRNA1-Fluc-Hyg This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-PASD-shRNA1-Fluc-Hyg plasmid Sequence

1 TTGGGGTTGC GCCTTTTCCA AGGCAGCCCT GGGTTTGCGC AGGGACGCGG CTGCTCTGGG 61 CGTGGTTCCG GGAAACGCAG CGGCGCCGAC CCTGGGTCTC GCACATTCTT CACGTCCGTT 121 CGCAGCGTCA CCCGGATCTT CGCCGCTACC CTTGTGGGCC CCCCGGCGAC GCTTCCTGCT 181 CCGCCCCTAA GTCGGGAAGG TTCCTTGCGG TTCGCGGCGT GCCGGACGTG ACAAACGGAA 241 GCCGCACGTC TCACTAGTAC CCTCGCAGAC GGACAGCGCC AGGGAGCAAT GGCAGCGCGC 301 CGACCGCGAT GGGCTGTGGC CAATAGCGGC TGCTCAGCAG GGCGCGCCGA GAGCAGCGGC 361 CGGGAAGGGG CGGTGCGGGA GGCGGGGTGT GGGGCGGTAG TGTGGGCCCT GTTCCTGCCC 421 GCGCGGTGTT CCGCATTCTG CAAGCCTCCG GAGCGCACGT CGGCAGTCGG CTCCCTCGTT 481 GACCGAATCA CCGACCTCTC TCCCCAGGGG GATCCATGAA GAAGCCCGAA CTCACCGCTA 541 CCAGCGTTGA AAAATTTCTC ATCGAGAAGT TCGACAGTGT GAGCGACCTG ATGCAGTTGT 601 CGGAGGGCGA AGAGAGCCGA GCCTTCAGCT TCGATGTCGG CGGACGCGGC TATGTACTGC 661 GGGTGAATAG CTGCGCTGAT GGCTTCTACA AAGACCGCTA CGTGTACCGC CACTTCGCCA 721 GCGCTGCACT ACCCATCCCC GAAGTGTTGG ACATCGGCGA GTTCAGCGAG AGCCTGACAT 781 ACTGCATCAG TAGACGCGCC CAAGGCGTTA CTCTCCAAGA CCTCCCCGAA ACAGAGCTGC 841 CTGCTGTGTT ACAGCCTGTC GCCGAAGCTA TGGATGCTAT TGCCGCCGCC GACCTCAGTC 901 AAACCAGCGG CTTCGGCCCA TTCGGGCCCC AAGGCATCGG CCAGTACACA ACCTGGCGGG 961 ATTTCATTTG CGCCATTGCT GATCCCCATG TCTACCACTG GCAGACCGTG ATGGACGACA 1021 CCGTGTCCGC CAGCGTAGCT CAAGCCCTGG ACGAACTGAT GCTGTGGGCC GAAGACTGTC 1081 CCGAGGTGCG CCACCTCGTC CATGCCGACT TCGGCAGCAA CAACGTCCTG ACCGACAACG 1141 GCCGCATCAC CGCCGTAATC GACTGGTCCG AAGCTATGTT CGGGGACAGT CAGTACGAGG 1201 TGGCCAACAT CTTCTTCTGG CGGCCCTGGC TGGCTTGCAT GGAGCAGCAG ACTCGCTACT 1261 TCGAGCGCCG GCATCCCGAG CTGGCCGGCA GCCCTCGTCT GCGAGCCTAC ATGCTGCGCA 1321 TCGGCCTGGA TCAGCTCTAC CAGAGCCTCG TGGACGGCAA CTTCGACGAT GCTGCCTGGG 1381 CTCAAGGCCG CTGCGATGCC ATCGTCCGCA GCGGGGCCGG CACCGTCGGT CGCACACAAA 1441 TCGCTCGCCG GAGCGCAGCC GTATGGACCG ACGGCTGCGT CGAGGTGCTG GCCGACAGCG 1501 GCAACCGCCG GCCCAGTACA CGACCGCGCG CTAAGGAGGT AGGTCGAGTT TAAGGTACCT 1561 TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG 1621 GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATC TGCTTTTTGC TTGTACTGGG 1681 TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG 1741 CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT 1801 GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT 1861 AGTAGTTCAT GTCATCTTAT TATTCAGTAT TTATAACTTG CAAAGAAATG AATATCAGAG 1921 AGTGAGAGGA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA 1981 AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC 2041 AATGTATCTT ATCATGTCTG GCTCTAGCTA TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 2101 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG 2161 CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 2221 GAGGCCTAGG GACGTACCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC 2281 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC 2341 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC 2401 CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGCG CATTAAGCGC 2461 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC 2521 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT 2581 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA 2641 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC 2701 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT 2761 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 2821 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT 2881 TACAATTTAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC 2941 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA 3001 TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT 3061 GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT 3121 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC 3181 CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA 3241 TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC 3301 TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC 3361 ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC 3421 TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG 3481 GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC 3541 GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC 3601 GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT 3661 GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA 3721 GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC 3781 CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG 3841 ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA 3901 TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC 3961 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA 4021 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC 4081 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA 4141 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT 4201 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC 4261 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG 4321 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG 4381 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG 4441 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC 4501 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT 4561 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG 4621 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC 4681 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT 4741 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA 4801 GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG 4861 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC 4921 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG 4981 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC 5041 CATGATTACG CCAAGCGCGC AATTAACCCT CACTAAAGGG AACAAAAGCT GGAGCTGCAA 5101 GCTTAATGTA GTCTTATGCA ATACTCTTGT AGTCTTGCAA CATGGTAACG ATGAGTTAGC 5161 AACATGCCTT ACAAGGAGAG AAAAAGCACC GTGCATGCCG ATTGGTGGAA GTAAGGTGGT 5221 ACGATCGTGC CTTATTAGGA AGGCAACAGA CGGGTCTGAC ATGGATTGGA CGAACCACTG 5281 AATTGCCGCA TTGCAGAGAT ATTGTATTTA AGTGCCTAGC TCGATACATA AACGGGTCTC 5341 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 5401 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 5461 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 5521 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 5581 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 5641 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 5701 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 5761 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 5821 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 5881 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 5941 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 6001 AAGTAAGACC ACCGCACAGC AAGCGGCCGC TGATCTTCAG ACCTGGAGGA GGAGATATGA 6061 GGGACAATTG GAGAAGTGAA TTATATAAAT ATAAAGTAGT AAAAATTGAA CCATTAGGAG 6121 TAGCACCCAC CAAGGCAAAG AGAAGAGTGG TGCAGAGAGA AAAAAGAGCA GTGGGAATAG 6181 GAGCTTTGTT CCTTGGGTTC TTGGGAGCAG CAGGAAGCAC TATGGGCGCA GCGTCAATGA 6241 CGCTGACGGT ACAGGCCAGA CAATTATTGT CTGGTATAGT GCAGCAGCAG AACAATTTGC 6301 TGAGGGCTAT TGAGGCGCAA CAGCATCTGT TGCAACTCAC AGTCTGGGGC ATCAAGCAGC 6361 TCCAGGCAAG AATCCTGGCT GTGGAAAGAT ACCTAAAGGA TCAACAGCTC CTGGGGATTT 6421 GGGGTTGCTC TGGAAAACTC ATTTGCACCA CTGCTGTGCC TTGGAATGCT AGTTGGAGTA 6481 ATAAATCTCT GGAACAGATT TGGAATCACA CGACCTGGAT GGAGTGGGAC AGAGAAATTA 6541 ACAATTACAC AAGCTTAATA CACTCCTTAA TTGAAGAATC GCAAAACCAG CAAGAAAAGA 6601 ATGAACAAGA ATTATTGGAA TTAGATAAAT GGGCAAGTTT GTGGAATTGG TTTAACATAA 6661 CAAATTGGCT GTGGTATATA AAATTATTCA TAATGATAGT AGGAGGCTTG GTAGGTTTAA 6721 GAATAGTTTT TGCTGTACTT TCTATAGTGA ATAGAGTTAG GCAGGGATAT TCACCATTAT 6781 CGTTTCAGAC CCACCTCCCA ACCCCGAGGG GACCCGACAG GCCCGAAGGA ATAGAAGAAG 6841 AAGGTGGAGA GAGAGACAGA GACAGATCCA TTCGATTAGT GAACGGATCT CGACGGTATC 6901 GATCACGAGA CTAGCCTCGA GCGGCCGCCC CCTTCACCGA GGGCCTATTT CCCATGATTC 6961 CTTCATATTT GCATATACGA TACAAGGCTG TTAGAGAGAT AATTGGAATT AATTTGACTG 7021 TAAACACAAA GATATTAGTA CAAAATACGT GACGTAGAAA GTAATAATTT CTTGGGTAGT 7081 TTGCAGTTTT AAAATTATGT TTTAAAATGG ACTATCATAT GCTTACCGTA ACTTGAAAGT 7141 ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AAACACCGGC AAGTGACGCT 7201 ACAGTTATTA ttcaagagaT AATAACTGTA GCGTCACTTG ttttttGAAT TCCGCGTTAC 7261 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC 7321 AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT 7381 GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC 7441 GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC 7501 CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT 7561 GATGCGGTTT TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC 7621 AAGTCTCCAC CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT 7681 TCCAAAATGT CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG 7741 GGAGGTCTAT ATAAGCAGAG CTAATCGTCG ACGATTCGTC AGAATCGGTC GCCACCatgg 7801 aagacgccaa aaacataaag aaaggcccgg cgccattcta tcctcttgag gatggaaccg 7861 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt 7921 ttacagatgc acatatcgag gtgaacatca cgtacgcgga atacttcgaa atgtccgttc 7981 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca 8041 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag 8101 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg aacatttcgc 8161 agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa 8221 aattaccaat aatccagaaa attattatca tggattctaa aacggattac cagggatttc 8281 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg 8341 taccagagtc ctttgatcgt gacaaaacaa ttgcactgat aatgaactcc tctggatcta 8401 ctgggttacc taagggtgtg gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg 8461 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc 8521 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag 8581 tcgtcttaat gtatagattt gaagaagagc tgtttttacg atcccttcag gattacaaaa 8641 ttcaaagtgc gttgctagta ccaaccctat tttcattctt cgccaaaagc actctgattg 8701 acaaatacga tttatctaat ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag 8761 aagtcgggga agcggttgca aaacgcttcc atcttccagg gatacgacaa ggatatgggc 8821 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg 8881 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc 8941 tgggcgttaa tcagagaggc gaattatgtg tcagaggacc tatgattatg tccggttatg 9001 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag 9061 acatagctta ctgggacgaa gacgaacact tcttcatagt tgaccgcttg aagtctttaa 9121 ttaaatacaa aggataccag gtggcccccg ctgaattgga gtcgatattg ttacaacacc 9181 ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga tgacgccggt gaacttcccg 9241 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg 9301 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag 9361 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg 9421 ccaagaaggg cggaaagtcc aaattgTAGT TCTCGACCTC GAGACAAATG GCAGTATTCA 9481 TCCACAATTT TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG 9541 ACATAATAGC AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA 9601 ATTTTCGGGT TTATTACAGG GACAGCAGAG ATCCACTTTG GCCGCGGCTC GAGGGGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.