pTac-GST-KLHL14 plasmid

pTac-GST-KLHL14 plasmid

PVT90214

pTac-GST-KLHL14 plasmid Information

Plasmid name: pTac-GST-KLHL14(human)
Alias: NM_020805.3
Gene lenght: 1887bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pGEX-5'
Reverse primer:
Copy number: High
Induction: IPTG Induction

pTac-GST-KLHL14 plasmid Description

pTac-GST-KLHL14 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-KLHL14 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatgtc cagatccggg gacaggacct 961 ccaccttcga ccccagccac agcgacaacc tgctgcacgg cctcaacctg ctgtggagga 1021 agcagctgtt ttgcgacgtg accctgacgg cccagggcca gcagttccat tgccacaagg 1081 ccgtgctggc ctcctgctcg cagtacttcc gatcgctctt ctccagccac ccccctctcg 1141 ggggaggggt cggcggccag gacggcctgg gggcccccaa ggaccagcag cagccgccgc 1201 agcagcagcc gtcacagcag cagcagccgc cgccgcagga ggagcccggg actccttctt 1261 cctcccccga cgacaagctg ctgaccagcc cccgggccat caacaacctg gtgctgcagg 1321 gctgctcgtc catcgggctg cgcctggtgc tcgagtacct ctacacggcc aacgtgaccc 1381 tgtccctgga cacggtggag gaggtgctgt cggtcagcaa gatcctgcac atcccccagg 1441 tcaccaagct ctgcgtgcag ttcctcaacg accagatctc ggtgcagaac tacaagcagg 1501 tgtgcaagat cgccgcgctg cacggcctgg aggagaccaa gaagctggcc aacaagtacc 1561 tggtggagga tgtgctgctg ctcaacttcg aggagatgcg cgccctgctg gactcgctgc 1621 cgccccccgt ggagtcggag ctggcgctct tccagatgtc cgtgctgtgg ctggagcacg 1681 accgcgagac ccgcatgcag tatgcgcctg acctcatgaa gcgcctccgc ttcgccctca 1741 tcccggcccc ggagctggtg gagcgggtcc agtcagtgga tttcatgcga accgacccgg 1801 tctgccagaa gctgctgctg gacgccatga actaccacct gatgcccttc aggcagcact 1861 gcaggcagag cctggccagc agaattcgct ctaacaagaa aatgctgtta ttggttggag 1921 ggctgcctcc tggaccggac cggctcccca gcaatttggt tcagtattac gacgatgaaa 1981 agaagacatg gaaaatactc acaattatgc catacaacag tgcccaccac tgcgttgtgg 2041 aggtggaaaa cttcttgttc gtgttgggtg gagaggacca gtggaatccg aatggaaaac 2101 acagtacaaa ttttgtcagc cgatatgatc ctcgatttaa tagctggatt caacttccac 2161 ccatgcagga aagaagagcc agtttctatg catgtcggtt ggacaagcat ttatacgtaa 2221 ttggtggaag gaatgaaact ggctacttgt ccagcgtgga gtgctataac ctagaaacga 2281 atgaatggcg ctatgtgtcc tctttgccac agcctctggc ggctcatgcg ggagcagtgc 2341 acaatgggaa aatatacatt tcagggggtg tacacaatgg agaatatgtc ccatggctat 2401 attgctatga cccagtaatg gatgtctggg ctcgaaaaca agatatgaac acaaaacgtg 2461 caattcacac tttggctgta atgaatgatc gcttgtatgc aattggagga aatcatttga 2521 aaggtttctc ccaccttgat gtaatgcttg tggaatgcta tgacccaaaa ggtgaccagt 2581 ggaatatact ccaaactccc attttggagg gtcgaagtgg ccctggctgt gcagtgcttg 2641 atgacagcat ttaccttgtg ggaggctaca gctggagtat gggggcctac aagtcatcta 2701 caatatgcta ttgtccagag aaaggaacct ggacagaact cgaaggagat gtagcagaac 2761 cgttggcagg ccctgcctgt gtgacagtta ttctgccctc ttgtgtacca tacaacaaat 2821 aactcgagcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg 2881 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 2941 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 3001 ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg 3061 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 3121 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 3181 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 3241 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 3301 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 3361 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 3421 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 3481 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 3541 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 3601 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 3661 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 3721 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 3781 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 3841 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 3901 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 3961 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 4021 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 4081 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 4141 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 4201 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 4261 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 4321 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 4381 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 4441 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 4501 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 4561 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 4621 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 4681 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 4741 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 4801 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 4861 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 4921 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 4981 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 5041 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 5101 taaattccga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg 5161 aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt 5221 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg 5281 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg 5341 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg 5401 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg 5461 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc 5521 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg 5581 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg 5641 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg 5701 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg 5761 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc 5821 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa 5881 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg 5941 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg 6001 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg 6061 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg 6121 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc 6181 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 6241 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 6301 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 6361 agcggataac aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt 6421 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 6481 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 6541 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt 6601 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa 6661 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt 6721 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt 6781 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttggaatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.