pCAMBIA1391-MCS-EGFP-Neo plasm

pCAMBIA1391-MCS-EGFP-Neo plasmid

PVT90824

pCAMBIA1391-MCS-EGFP-Neo plasmid Information

Plasmid name: pCAMBIA1391-MCS-EGFP-Neo
Alias: -
Gene lenght:
Host: Plant
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: 35S
Replicon: pVS1
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pEGFP-N-3'
Copy number: High
Induction:

pCAMBIA1391-MCS-EGFP-Neo plasmid Description

pCAMBIA1391-MCS-EGFP-Neo This is a plasmid suitable for protein expression in plants.

pCAMBIA1391-MCS-EGFP-Neo plasmid Sequence

1 gtggactcct cttagaattc ccggatccgt cgacctgcag ccaagctttg agacttttca 61 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat 121 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa 181 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag 241 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga 301 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag 361 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc 421 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca 481 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc 541 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt 601 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc 661 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac 721 gctgaaatca ccagtctctc tctacaaatc tatctctctc gagctttcgc agatctgtcg 781 atcgaccatg gggattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 841 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt 901 tcggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 961 gaatgaactc caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 1021 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 1081 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 1141 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 1201 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 1261 tctggacgaa gaacatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 1321 catgcccgac ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc cgaatatcat 1381 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 1441 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gaacttggcg gcgaatgggc 1501 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 1561 tcgccttctt gacgagttct tctgactcga gtttctccat aataatgtgt gagtagttcc 1621 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc 1681 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac 1741 caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtacatta 1801 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata 1861 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg 1921 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga 1981 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac 2041 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg 2101 tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa 2161 caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt 2221 ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga 2281 aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa 2341 taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga 2401 gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat 2461 gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc 2521 ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc 2581 ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg 2641 gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta 2701 gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc 2761 gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag 2821 acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc 2881 tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac 2941 aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag 3001 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat 3061 tatattttac tggatgaatt gttttagtac ctagaatgca tgaccaaaat cccttaacgt 3121 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 3181 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 3241 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 3301 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 3361 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 3421 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 3481 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 3541 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 3601 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 3661 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 3721 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 3781 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 3841 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 3901 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat 3961 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc 4021 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca 4081 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc 4141 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt 4201 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt 4261 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt 4321 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac 4381 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag 4441 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct 4501 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg 4561 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta 4621 tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc 4681 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg 4741 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt 4801 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg 4861 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc 4921 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc 4981 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc 5041 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct 5101 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca 5161 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca 5221 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc 5281 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc 5341 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg 5401 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga 5461 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc 5521 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg 5581 gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca 5641 ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc 5701 tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg 5761 ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc 5821 agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg 5881 tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca 5941 tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta 6001 atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac 6061 tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg 6121 ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct 6181 ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc 6241 gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg 6301 ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg 6361 cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg 6421 cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg 6481 tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga 6541 tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc 6601 ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg 6661 gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta 6721 acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct 6781 tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat 6841 ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc 6901 aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg 6961 ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga 7021 gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc 7081 ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg 7141 cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca 7201 ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg 7261 gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg 7321 atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc 7381 acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc 7441 tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg 7501 tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca 7561 atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg 7621 tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg 7681 gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg 7741 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc 7801 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa 7861 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac 7921 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta 7981 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat 8041 gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat ccgttattct 8101 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt 8161 taattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg 8221 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac 8281 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa 8341 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta 8401 ttgccaaatg tttgaacgat cggggaaatt cgagctggtc accaattcac acgtggtggt 8461 ggtggtggtg gctagccttg tacagctcgt ccatgccgtg agtgatcccg gcggcggtca 8521 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact 8581 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct 8641 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt 8701 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt 8761 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct 8821 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc 8881 cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga 8941 agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtga 9001 aggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca 9061 gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc 9121 cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc 9181 ccttgctcac catactagtc agatctacca tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.