pLV3-CMV-ANXA3-3×FLAG-CopGFP-P

pLV3-CMV-ANXA3-3×FLAG-CopGFP-Puro plasmid

PVT90184

pLV3-CMV-ANXA3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-ANXA3(human)-3×FLAG-CopGFP-Puro
Alias: NM_005139.3;ANX3
Gene lenght: 969bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ANXA3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-ANXA3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-ANXA3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg catctatctg ggttggacac 2341 cgaggaacag taagagatta tccagacttt agcccatcag tggatgctga agctattcag 2401 aaagcaatca gaggaattgg aactgatgag aaaatgctca tcagcattct gactgagagg 2461 tcaaatgcac agcggcagct gattgttaag gaatatcaag cagcatatgg aaaggagctg 2521 aaagatgact tgaagggtga tctctctggc cactttgagc atctcatggt ggccctagtg 2581 actccaccag cagtctttga tgcaaagcag ctaaagaaat ccatgaaggg cgcgggaaca 2641 aacgaagatg ccttgattga aatcttaact accaggacaa gcaggcaaat gaaggatatc 2701 tctcaagcct attatacagt atacaagaag agtcttggag atgacattag ttccgaaaca 2761 tctggtgact tccggaaagc tctgttgact ttggcagatg gcagaagaga tgaaagtctg 2821 aaagtggatg agcatctggc caaacaagat gcccagattc tctataaagc tggtgagaac 2881 agatggggca cggatgaaga caaattcact gagatcctgt gtttaaggag ctttcctcaa 2941 ttaaaactaa catttgatga atacagaaat atcagccaaa aggacattgt ggacagcata 3001 aaaggagaat tatctgggca ttttgaagac ttactgttgg ccatagttaa ttgtgtgagg 3061 aacacgccgg cctttttagc cgaaagactg catcgagcct tgaagggtat tggaactgat 3121 gagtttactc tgaaccgaat aatggtgtcc agatcagaaa ttgacctttt ggacattcga 3181 acagagttca agaagcatta tggctattcc ctatattcag caattaaatc ggatacttct 3241 ggagactatg aaatcacact cttaaaaatc tgtggtggag atgacggatc cGACTACAAA 3301 GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGtag 3361 cggccgcGaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 3421 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 3481 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 3541 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 3601 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 3661 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 3721 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 3781 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 3841 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 3901 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg 3961 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 4021 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 4081 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 4141 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 4201 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 4261 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 4321 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 4381 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 4441 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 4501 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 4561 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 4621 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 4681 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 4741 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 4801 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 4861 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 4921 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 4981 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 5041 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 5101 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 5161 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 5221 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 5281 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat 5341 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 5401 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 5461 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 5521 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 5581 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 5641 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 5701 ggcactgaca attccgtggt gttgtcgggg aaATCATCGT CCTTTCCTtg gctgctcgcc 5761 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 5821 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctTcgc 5881 cttcgccctc agacgagtcg gatctccctt tggGccgcct ccccgcctgg tacctttaag 5941 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 6001 ggaagggcta attcactccc aacgaaGata agatctgctt tttgcttgta ctgggtctct 6061 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 6121 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 6181 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 6241 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 6301 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 6361 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 6421 atcttatcat gtctggctct agctaTCCCG CCCCTAACTC CGCCCAtccc gcccctaact 6481 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 6541 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 6601 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg 6661 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 6721 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 6781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 6841 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 6901 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 6961 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 7021 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 7081 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 7141 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 7201 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 7261 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 7321 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 7381 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 7441 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 7501 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 7561 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 7621 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 7681 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7741 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7801 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7861 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 7921 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 7981 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 8041 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8101 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8161 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8221 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8281 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8341 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8401 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8461 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8521 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8581 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8641 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8701 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8761 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 8821 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 8881 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 8941 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 9001 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 9061 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 9121 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 9181 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 9241 gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.