pLV3-CMV-HADH-3×FLAG-CopGFP-Pu

pLV3-CMV-HADH-3×FLAG-CopGFP-Puro plasmid

PVT90750

pLV3-CMV-HADH-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HADH(human)-3×FLAG-CopGFP-Puro
Alias: NM_001184705.4;HAD; HADH1; HADHSC; HCDH; HHF4; MSCHAD;SCHAD
Gene lenght: 993bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HADH-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-HADH-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-HADH-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ccttcgtcac caggcagttc 2341 atgcgttccg tgtcctcctc gtccaccgcc tcggcctcgg ccaagaagat aatcgtcaag 2401 cacgtgacgg tcatcggcgg cgggctgatg ggcgccggca ttgcccaggt tgctgcagca 2461 actggtcaca cagtagtgtt ggtagaccag acagaggaca tcctggcaaa atccaaaaag 2521 ggaattgagg aaagccttag gaaagtggca aagaagaagt ttgcagaaaa ccttaaggcc 2581 ggcgatgaat ttgtggagaa gaccctgagc accatagcga ccagcacgga tgcagcctcc 2641 gttgtccaca gcacagactt ggtggtggaa gccatcgtgg agaatctgaa ggtgaaaaac 2701 gagctcttca aaaggctgga caagtttgct gctgaacata caatctttgc cagcaacact 2761 tcctccttgc agattacaag catagctaat gccaccacca gacaagaccg attcgctggc 2821 ctccatttct tcaacccagt gcctgtcatg aaacttgtgg aggtcattaa aacaccaatg 2881 accagccaga agacatttga atctttggta gactttagca aagccctagg aaagcatcct 2941 gtttcttgca aggacactcc tgggtttatt gtgaaccgcc tcctggttcc atacctcatg 3001 gaagcaatca ggctgtatga acgagacttc caaacgtgtg gtgattctaa ctcgggtttg 3061 ggcttttctt taaaaggtga cgcatccaaa gaagacattg acactgctat gaaattagga 3121 gccggttacc ccatgggccc atttgagctt ctagattatg tcggactgga tactacgaag 3181 ttcatcgtgg atgggtggca tgaaatggat gcagagaacc cattacatca gcccagccca 3241 tccttaaata agctggtagc agagaacaag ttcggcaaga agactggaga aggattttac 3301 aaatacaagg gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC 3361 TACAAGGATG ACGATGACAA Gtagcggccg cGaaggatct gcgatcgctc cggtgcccgt 3421 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 3481 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 3541 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 3601 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc 3661 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt 3721 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag 3781 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg 3841 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt 3901 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc 3961 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc 4021 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac 4081 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg 4141 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac 4201 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg 4261 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg 4321 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac 4381 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc 4441 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc 4501 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc 4561 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag 4621 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc 4681 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac 4741 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc 4801 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 4861 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 4921 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 4981 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 5041 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 5101 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 5161 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 5221 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 5281 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 5341 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact 5401 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 5461 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 5521 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 5581 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 5641 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 5701 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaATCA 5761 TCGTCCTTTC CTtggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 5821 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 5881 ctgcggcctc ttccgcgtct Tcgccttcgc cctcagacga gtcggatctc cctttggGcc 5941 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 6001 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa Gataagatct 6061 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 6121 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 6181 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 6241 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 6301 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 6361 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 6421 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctaT CCCGCCCCTA 6481 ACTCCGCCCA tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 6541 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 6601 tagtgaggag gcttttttgg aggcctagac ttttgcagag accaaattcg taatcatgtc 6661 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 6721 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 6781 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 6841 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 6901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 6961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 7021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 7081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 7141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 7201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 7261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 7321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 7381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 7441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 7501 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 7561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 7621 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 7681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 7741 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 7801 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 7861 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 7921 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 7981 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 8041 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 8101 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 8161 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 8221 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 8281 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 8341 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 8401 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 8461 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 8521 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 8581 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 8641 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 8701 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 8761 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 8821 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 8881 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 8941 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 9001 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 9061 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca 9121 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 9181 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 9241 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.