pLV2-CMV-3×FLAG-HMGB2-Puro pla

pLV2-CMV-3×FLAG-HMGB2-Puro plasmid

PVT90612

pLV2-CMV-3×FLAG-HMGB2-Puro plasmid Information

Plasmid name: pLV2-CMV-3×FLAG-HMGB2(human)-Puro
Alias: NM_002129.4;HMG2
Gene lenght: 630bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-3×FLAG-HMGB2-Puro plasmid Description

pLV2-CMV-3×FLAG-HMGB2-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-3×FLAG-HMGB2-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgaG 2821 CCACCATGGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG 2881 ATGACGATGA CAAGgaattc atgggtaaag gagaccccaa caagccgcgg ggcaaaatgt 2941 cctcgtacgc cttcttcgtg cagacctgcc gggaagagca caagaagaaa cacccggact 3001 cttccgtcaa tttcgcggaa ttctccaaga agtgttcgga gagatggaag accatgtctg 3061 caaaggagaa gtcgaagttt gaagatatgg caaaaagtga caaagctcgc tatgacaggg 3121 agatgaaaaa ttacgttcct cccaaaggtg ataagaaggg gaagaaaaag gaccccaatg 3181 ctcctaaaag gccaccatct gccttcttcc tgttttgctc tgaacatcgc ccaaagatca 3241 aaagtgaaca ccctggccta tccattgggg atactgcaaa gaaattgggt gaaatgtggt 3301 ctgagcagtc agccaaagat aaacaaccat atgaacagaa agcagctaag ctaaaggaga 3361 aatatgaaaa ggatattgct gcatatcgtg ccaagggcaa aagtgaagca ggaaagaagg 3421 gccctggcag gccaacaggc tcaaagaaga agaacgaacc agaagatgag gaggaggagg 3481 aggaagaaga agatgaagat gaggaggaag aggatgaaga tgaagaataa ggatcccgcg 3541 actctagata attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg 3601 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt 3661 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt 3721 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt 3781 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat 3841 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg 3901 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg 3961 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg 4021 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat 4081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg 4141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg 4201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 4261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 4321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 4381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 4441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 4501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 4561 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 4621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc 4681 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 4741 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 4801 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 4861 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 4921 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 4981 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 5041 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc 5101 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 5161 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 5221 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 5281 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac 5341 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt 5401 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 5461 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga 5521 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac 5581 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt 5641 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt 5701 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg 5761 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata 5821 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg 5881 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg 5941 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 6001 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 6061 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 6121 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 6181 agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg 6241 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 6301 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 6361 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 6421 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 6481 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 6541 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 6601 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 6661 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 6721 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 6781 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 6841 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 6901 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 6961 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 7021 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 7081 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 7141 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 7201 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 7261 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 7321 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 7381 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 7441 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 7501 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 7561 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 7621 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 7681 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 7741 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 7801 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 7861 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 7921 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 7981 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 8041 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 8101 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 8161 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 8221 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 8281 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 8341 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 8401 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag 8461 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 8521 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca 8581 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca 8641 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta 8701 aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca 8761 aacttagtag t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.