pLV3-CMV-LUZP1-3×HA-CopGFP-Pur

pLV3-CMV-LUZP1-3×HA-CopGFP-Puro plasmid

PVT90587

pLV3-CMV-LUZP1-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-LUZP1(human)-3×HA-CopGFP-Puro
Alias: NM_001142546.4;LUZP
Gene lenght: 3228bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-LUZP1-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-LUZP1-3×HA-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-LUZP1-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg ccgaatttac aagctacaag 2341 gagacggcct ccagccgcca cttgcggttt aagctacaga gtctaagccg ccgccttgat 2401 gagttggagg aagccacaaa aaacctccag aaagcagagg atgaactcct ggatctccag 2461 gacaaggtga ttcaggcaga aggtagcaac tccagcatgt tggcggagat tgaagtgctg 2521 cgccagcggg tgctgagaat tgaaggcaaa gacgaggaaa ttaagagagc agaggatctg 2581 tgtcgtctga tgaaggagaa acttgaagag gaagaaaacc tcacccggga gctgaaatct 2641 gagattgagc ggcttcagaa acgaatggct gaattagaga agctagaaga ggccttcagc 2701 aggagtaaga atgactgtac ccagctgtgt ctgagcctga atgaggagag aaatctgacc 2761 aagaaaatct cctctgagct ggaaatgctc agagtcaaag tgaaagaact agaatcttct 2821 gaggaccgcc tggataaaac tgagcagagt ttagcgtcag agttagaaaa gctgaaatca 2881 ttaactctga gctttgtaag tgagagaaaa tacttgaatg aaaaggagaa agaaaatgag 2941 aaattgataa aagaactcac tcaaaaacta gagcagaaca aaaaaatgaa ccgagattat 3001 acaaggaatg cttctaatct ggaaagaaat gacctacgga ttgaggatgg catctcttcc 3061 acactgccgt ccaaagaatc aagaaggaag ggtggtctgg actacctaaa gcaggtagag 3121 aatgaaacaa gaaacaaatc agaaaatgaa aagaaccgca atcaggaaga caacaaagtc 3181 aaagacctta accaagagat tgagaaactt aagacacaaa tcaaacactt tgaatcgttg 3241 gaagaagagc ttaagaaaat gaagtccaaa aataacgacc ttcaggataa ttacctaagt 3301 gaacaaaata aaaacaaatt attagccagc caactggagg agataaagct acaaatcaag 3361 aaacagaaag agttagaaaa tggagaggta gaaggggaag atgctttcct gtccagcaaa 3421 ggcagacatg agaggactaa gtttagaggc cacggaagtg aagcttctgt gtccaagcac 3481 acagcgcggg aactgtctcc tcagcataag cgggaacgac tccggaacag ggagtttgct 3541 ctcaacaatg aaaactattc tctgagcaac aggcaggttt cttctcccag tttcaccaac 3601 aggagggcag caaaagcttc tcacatgggg gtgagtacag acagtgggac tcaggagaca 3661 aagaaaactg aagaccggtt tgtacccggc tcctcccaga gcgaagggaa gaagtctagg 3721 gagcagccct cagtgctgag tcgctacccc ccggctgctc aggagcacag taaagcgtgg 3781 aaggggactt ccaagccagg caccgagagc ggactgaagg gaaaagtgga gaagacaaca 3841 cgaacgttta gtgacaccac ccatggatct gttcccagtg acccattggg tagagctgac 3901 aaggcttctg acacctcctc tgagactgtc tttggcaaga ggggacacgt gcttggcaac 3961 ggaagtcaag taactcaggc tgcaaactct ggctgttcta aggccattgg agccctggcc 4021 tcatctcgaa gatcctcctc agaagggctc tctaaaggca aaaaggctgc caatggcctt 4081 gaggctgata acagttgccc gaattccaag gctcctgttc tatcgaagta tccttatagc 4141 tgtagaagcc aagagaacat ccttcaggga ttttcaacct cacataaaga aggggttaat 4201 caacctgcag cagttgtgat ggaagacagc agtccgcatg aagccttgag gtgtcgagtc 4261 atcaaatcca gtggcagaga gaagccagac tcagatgatg acttggacat agcatctctt 4321 gttactgcca agttggtaaa tacaaccatc actccagagc cagagcccaa accacagcct 4381 aactctagag aaaaggctaa aacccgaggg gcacctagaa cctccctatt tgagaatgat 4441 aaagatgctg gaatggagaa tgaatctgtg aaatctgtca gagcctccac caataccatg 4501 gagctcccag ataccaatgg tgctggggta aaaagccaaa ggccctttag ccccagagag 4561 gcgttgcggt ctagagccat catcaaacct gttattgttg ataaggatgt gaaaaaaatc 4621 atgggaggat ctggaacaga gactacgttg gagaaacaga aacccgtctc caaaccaggg 4681 ccaaacaaag tgacaagtag cattactata tatccatctg acagcagcag ccccagagct 4741 gctccgggtg aggccctgag ggagaggcac acatccacta gcaatatcca ggtggggctg 4801 gcagagctca catcagttag caaccatgtc agctcccctt ttgagctctc cattcacaaa 4861 catgacatca ccctgcagct tgcagaagcg gagagaatgg cagatgggcc cctgaaggac 4921 aggccagaaa cagtggtctc tcggagcagc attataatca agccatcgga tcctgtggag 4981 aggaatagcc atgcacctcc agcggagaca atcaggtgga aaagccatag tgccccttca 5041 gaagtgggct tctcagatgc cagacatgtt actgtgcgga atgcctggaa gagtaggcga 5101 gacttgaaat ctttagaaga ccccccaact cgaataggta aaaacgtgga atctaccaat 5161 agcaatgcct acacccagag gtcttccaca gacttctcag aactcgaaca gcccaggtcc 5221 tgcctttttg agcagggcac tcgaagggta ggaccaagtt caggggatgc ccctgagccc 5281 tcctccagaa ggacccaaag tagcctcact gtgtcagagg tgcttacccg tcggaatcgg 5341 gtaggagaca ccatcactgt cgcagcctgg aaccactcag caagcatgga ggaagaaggg 5401 gaagactgta cactcagtgt ctacaggcaa ctgcacaact ccctggatcc gtctgaactg 5461 cctgggaagc aggggctgcc agagtctggg cgagtacggg ccgaggaacg attacggcca 5521 accaggccct gtgctgagga gaacCTCGAG TACCCATACG ACGTACCTGA TTATGCAACT 5581 TATCCTTATG ATGTACCTGA TTATGCTAGC TATCCTTATG ATGTCCCAGA CTACGCATAG 5641 ggatccCTGC GATATCCAGC gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 5701 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 5761 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 5821 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 5881 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 5941 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 6001 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 6061 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 6121 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 6181 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 6241 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 6301 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 6361 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 6421 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 6481 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 6541 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 6601 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 6661 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 6721 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 6781 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 6841 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 6901 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 6961 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 7021 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 7081 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 7141 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 7201 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 7261 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 7321 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 7381 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 7441 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 7501 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 7561 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 7621 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 7681 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 7741 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 7801 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 7861 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 7921 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 7981 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 8041 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 8101 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 8161 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct 8221 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 8281 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt 8341 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 8401 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 8461 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 8521 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 8581 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 8641 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 8701 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 8761 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 8821 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 8881 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 8941 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 9001 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 9061 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 9121 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 9181 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 9241 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 9301 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 9361 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 9421 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 9481 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 9541 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 9601 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 9661 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 9721 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 9781 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 9841 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 9901 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 9961 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 10021 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 10081 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 10141 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 10201 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 10261 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 10321 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 10381 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 10441 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 10501 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 10561 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 10621 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 10681 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 10741 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 10801 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 10861 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 10921 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 10981 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 11041 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 11101 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 11161 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 11221 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 11281 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 11341 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 11401 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 11461 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 11521 aaacgacggc cagtgccaag ctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.