pLV3-EF1a-MCS-PGK-Puro plasmid

pLV3-EF1a-MCS-PGK-Puro plasmid

PVT95889

pLV3-EF1a-MCS-PGK-Puro plasmid Information

Plasmid name: pLV3-EF1a-MCS-PGK-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-EF1a-MCS-PGK-Puro plasmid Description

pLV3-EF1a-MCS-PGK-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-EF1a-MCS-PGK-Puro plasmid Sequence

1 acgcgtTGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT 61 CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 121 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA 181 ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA 241 GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG 301 CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC 361 TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT 421 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT 481 TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG 541 ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGCGC GTTTTGCCTG 601 TACTgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 661 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 721 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 781 tagcagtggc gcccgaacag ggacctgaaa gcgaaaggga aaccagagct ctctcgacgc 841 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg 901 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt 961 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa 1021 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat 1081 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc 1141 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt 1201 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag 1261 caaaacaaaa gtaagaccac cgcacagcaa gcggccactg atcttcagac ctggaggagg 1321 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc 1381 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt 1441 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc 1501 ctcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa 1561 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat 1621 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct 1681 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag 1741 ttggagtaat aaatctctgg aacagattgg aatcacacga cctggatgga gtgggacaga 1801 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 1861 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 1921 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 1981 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 2041 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 2101 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga 2161 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta 2221 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa 2281 aattttatcg atactagtaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg 2341 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta 2401 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc 2461 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa 2521 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc 2581 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc 2641 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc 2701 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg 2761 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag 2821 atccaagctg tgaccggcgc ctactctaga gctagcgaat tcgaaggatc cgcggccgct 2881 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg 2941 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc 3001 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca 3061 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac 3121 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt 3181 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg 3241 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct 3301 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt 3361 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac 3421 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 3481 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 3541 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 3601 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 3661 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 3721 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 3781 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 3841 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 3901 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 3961 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc aacctctgga 4021 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 4081 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 4141 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 4201 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 4261 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 4321 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 4381 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac 4441 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 4501 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca 4561 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt 4621 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 4681 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 4741 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 4801 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 4861 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 4921 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 4981 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 5041 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 5101 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 5161 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 5221 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta 5281 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 5341 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 5401 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 5461 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 5521 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 5581 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 5641 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 5701 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 5761 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 5821 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 5881 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 5941 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 6001 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 6061 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 6121 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 6181 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 6241 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 6301 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 6361 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 6421 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 6481 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 6541 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 6601 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 6661 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 6721 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 6781 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 6841 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 6901 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 6961 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 7021 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 7081 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 7141 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 7201 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 7261 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 7321 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 7381 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 7441 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 7501 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 7561 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 7621 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 7681 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 7741 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 7801 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 7861 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.