pLV3-CMV-RBP5-3×FLAG-CopGFP-Pu

pLV3-CMV-RBP5-3×FLAG-CopGFP-Puro plasmid

PVT90569

pLV3-CMV-RBP5-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-RBP5(human)-3×FLAG-CopGFP-Puro
Alias: NM_031491.4;CRBP-III; CRBP3; CRBPIII; HRBPiso
Gene lenght: 405bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-RBP5-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-RBP5-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-RBP5-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc ctcccaacct cactggctac 2341 taccgctttg tctcgcagaa gaacatggag gactacctgc aagccctaaa catcagcttg 2401 gctgtgcgga agatcgcgct gctgctgaag ccggacaagg agatcgaaca ccagggcaac 2461 cacatgacgg tgaggacgct cagcaccttc cgaaactaca ctgtgcagtt tgatgtggga 2521 gtggagtttg aggaggacct caggagcgtg gacggacgaa aatgccagac catagtaacc 2581 tgggaggagg agcacctggt gtgtgtgcag aaaggggagg tccccaaccg gggctggaga 2641 cactggctgg agggagagat gctgtatctg gaactgactg caagggatgc agtgtgcgag 2701 caggtcttca ggaaggtcag aggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 2761 CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc 2821 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 2881 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 2941 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 3001 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 3061 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 3121 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 3181 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 3241 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 3301 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 3361 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc 3421 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc 3481 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg 3541 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac 3601 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag 3661 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc 3721 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc 3781 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc 3841 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc 3901 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc 3961 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag 4021 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc 4081 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta 4141 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc 4201 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 4261 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 4321 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 4381 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 4441 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 4501 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 4561 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 4621 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 4681 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 4741 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt 4801 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 4861 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 4921 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 4981 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 5041 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 5101 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 5161 tcggggaaAT CATCGTCCTT TCCTtggctg ctcgcctgtg ttgccacctg gattctgcgc 5221 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 5281 ctgctgccgg ctctgcggcc tcttccgcgt ctTcgccttc gccctcagac gagtcggatc 5341 tccctttggG ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 5401 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 5461 aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 5521 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 5581 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 5641 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 5701 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 5761 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 5821 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 5881 aTCCCGCCCC TAACTCCGCC CAtcccgccc ctaactccgc ccagttccgc ccattctccg 5941 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 6001 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt 6061 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 6121 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 6181 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 6241 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 6301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 6361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 6421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 6481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 6541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 6601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 6661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 6721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 6781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 6841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 6901 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 6961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 7021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 7081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 7141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 7201 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 7261 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 7321 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 7381 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 7441 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 7501 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 7561 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 7621 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 7681 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 7741 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 7801 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 7861 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 7921 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 7981 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 8041 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 8101 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 8161 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 8221 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 8281 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 8341 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 8401 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 8461 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 8521 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 8581 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 8641 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.