pLV3-CMV-HMGCL-3×FLAG-CopGFP-P

pLV3-CMV-HMGCL-3×FLAG-CopGFP-Puro plasmid

PVT95472

pLV3-CMV-HMGCL-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HMGCL(human)-3×FLAG-CopGFP-Puro
Alias: NM_000191.3;HL; HMGCL1
Gene lenght: 975bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HMGCL-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-HMGCL-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-HMGCL-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cagcaatgag gaaggcgctt 2341 ccgcggcgac tggtgggctt ggcgtccctc cgggctgtca gcacctcatc tatgggcact 2401 ttaccaaagc gggtgaaaat tgtggaagtt ggtccccgag atggactaca aaatgaaaag 2461 aatatcgtat ctactccagt gaaaatcaag ctgatagaca tgctttctga agcaggactc 2521 tctgttatag aaaccaccag ctttgtgtct cctaagtggg ttccccagat gggtgaccac 2581 actgaagtct tgaagggcat tcagaagttt cctggcatca actacccagt cctgacccca 2641 aatttgaaag gcttcgaggc agcggttgct gctggagcca aggaagtagt catctttgga 2701 gctgcctcag agctcttcac caagaagaac atcaattgtt ccatagagga gagttttcag 2761 aggtttgacg caatcctgaa ggcagcgcag tcagccaata tttctgtgcg ggggtacgtc 2821 tcctgtgctc ttggctgccc ttatgaaggg aagatctccc cagctaaagt agctgaggtc 2881 accaagaagt tctactcaat gggctgctac gagatctccc tgggggacac cattggtgtg 2941 ggcaccccag ggatcatgaa agacatgcta tctgctgtca tgcaggaagt gcctctggct 3001 gccctggctg tccactgcca tgacacctat ggtcaagccc tggccaacac cttgatggcc 3061 ctgcagatgg gagtgagtgt cgtggactct tctgtggcag gacttggagg ctgtccctac 3121 gcacaggggg catcaggaaa cttggccaca gaagacctgg tctacatgct agagggcttg 3181 ggcattcaca cgggtgtgaa tctccagaag cttctggaag ctggaaactt tatctgtcaa 3241 gccctgaaca gaaaaactag ctccaaagtg gctcaggcta cctgtaaact cggatccGAC 3301 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 3361 AAGtagcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca 3421 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga 3481 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 3541 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 3601 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc 3661 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt 3721 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc 3781 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg 3841 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc 3901 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc 3961 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg 4021 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa 4081 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac 4141 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc 4201 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc 4261 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc 4321 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg 4381 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac 4441 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc 4501 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc 4561 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc 4621 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc 4681 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 4741 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 4801 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 4861 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 4921 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 4981 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 5041 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 5101 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 5161 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 5221 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 5281 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 5341 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 5401 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 5461 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 5521 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 5581 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 5641 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 5701 ctgttgggca ctgacaattc cgtggtgttg tcggggaaAT CATCGTCCTT TCCTtggctg 5761 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 5821 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 5881 ctTcgccttc gccctcagac gagtcggatc tccctttggG ccgcctcccc gcctggtacc 5941 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 6001 gggactggaa gggctaattc actcccaacg aaGataagat ctgctttttg cttgtactgg 6061 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 6121 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 6181 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 6241 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 6301 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 6361 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 6421 caatgtatct tatcatgtct ggctctagct aTCCCGCCCC TAACTCCGCC CAtcccgccc 6481 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 6541 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 6601 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg 6661 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 6721 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 6781 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 6841 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 6901 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 6961 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 7021 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 7081 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 7141 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 7201 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 7261 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7321 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7381 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7441 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 7501 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7561 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7621 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 7681 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 7741 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 7801 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 7861 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 7921 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 7981 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 8041 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 8101 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 8161 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 8221 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 8281 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 8341 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 8401 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 8461 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 8521 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 8581 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 8641 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 8701 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 8761 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 8821 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 8881 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 8941 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 9001 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 9061 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 9121 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 9181 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 9241 taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.