pCMV-3×FLAG-PKM-1-Neo plasmid

pCMV-3×FLAG-PKM-1-Neo plasmid

PVT93956

pCMV-3×FLAG-PKM-1-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-PKM(human)-1-Neo
Alias: NM_002654.6;CTHBP; HEL-S-30; OIP3; p58; PK3; PKM2; TCB;THBP1
Gene lenght: 1596bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-PKM-1-Neo plasmid Description

pCMV-3×FLAG-PKM-1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-PKM-1-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atgtcgaagc cccatagtga agccgggact 1021 gccttcattc agacccagca gctgcacgca gccatggctg acacattcct ggagcacatg 1081 tgccgcctgg acattgattc accacccatc acagcccgga acactggcat catctgtacc 1141 attggcccag cttcccgatc agtggagacg ttgaaggaga tgattaagtc tggaatgaat 1201 gtggctcgtc tgaacttctc tcatggaact catgagtacc atgcggagac catcaagaat 1261 gtgcgcacag ccacggaaag ctttgcttct gaccccatcc tctaccggcc cgttgctgtg 1321 gctctagaca ctaaaggacc tgagatccga actgggctca tcaagggcag cggcactgca 1381 gaggtggagc tgaagaaggg agccactctc aaaatcacgc tggataacgc ctacatggaa 1441 aagtgtgacg agaacatcct gtggctggac tacaagaaca tctgcaaggt ggtggaagtg 1501 ggcagcaaga tctacgtgga tgatgggctt atttctctcc aggtgaagca gaaaggtgcc 1561 gacttcctgg tgacggaggt ggaaaatggt ggctccttgg gcagcaagaa gggtgtgaac 1621 cttcctgggg ctgctgtgga cttgcctgct gtgtcggaga aggacatcca ggatctgaag 1681 tttggggtcg agcaggatgt tgatatggtg tttgcgtcat tcatccgcaa ggcatctgat 1741 gtccatgaag ttaggaaggt cctgggagag aagggaaaga acatcaagat tatcagcaaa 1801 atcgagaatc atgagggggt tcggaggttt gatgaaatcc tggaggccag tgatgggatc 1861 atggtggctc gtggtgatct aggcattgag attcctgcag agaaggtctt ccttgctcag 1921 aagatgatga ttggacggtg caaccgagct gggaagcctg tcatctgtgc tactcagatg 1981 ctggagagca tgatcaagaa gccccgcccc actcgggctg aaggcagtga tgtggccaat 2041 gcagtcctgg atggagccga ctgcatcatg ctgtctggag aaacagccaa aggggactat 2101 cctctggagg ctgtgcgcat gcagcacctg attgcccgtg aggcagaggc tgccatctac 2161 cacttgcaat tatttgagga actccgccgc ctggcgccca ttaccagcga ccccacagaa 2221 gccaccgccg tgggtgccgt ggaggcctcc ttcaagtgct gcagtggggc cataatcgtc 2281 ctcaccaagt ctggcaggtc tgctcaccag gtggccagat accgcccacg tgcccccatc 2341 attgctgtga cccggaatcc ccagacagct cgtcaggccc acctgtaccg tggcatcttc 2401 cctgtgctgt gcaaggaccc agtccaggag gcctgggctg aggacgtgga cctccgggtg 2461 aactttgcca tgaatgttgg caaggcccga ggcttcttca agaagggaga tgtggtcatt 2521 gtgctgaccg gatggcgccc tggctccggc ttcaccaaca ccatgcgtgt tgttcctgtg 2581 ccgtgagaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt 2641 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc 2701 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat 2761 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg 2821 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc 2881 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc 2941 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 3001 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 3061 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 3121 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 3181 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 3241 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 3301 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 3361 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 3421 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 3481 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 3541 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 3601 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 3661 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 3721 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 3781 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 3841 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 3901 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 3961 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 4021 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 4081 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 4141 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 4201 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 4261 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 4321 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 4381 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 4441 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 4501 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 4561 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 4621 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 4681 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 4741 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 4801 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 4861 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 4921 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 4981 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 5041 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 5101 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 5161 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 5221 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 5281 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 5341 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 5401 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 5461 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 5521 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 5581 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 5641 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 5701 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 5761 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 5821 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag 5881 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 5941 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 6001 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 6061 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 6121 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 6181 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 6241 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 6301 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 6361 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 6421 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 6481 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 6541 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 6601 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 6661 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 6721 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 6781 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 6841 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 6901 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 6961 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 7021 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.