pLV3-CMV-SCARF2-EF1a-CopGFP-Pu

pLV3-CMV-SCARF2-EF1a-CopGFP-Puro plasmid

PVT96310

pLV3-CMV-SCARF2-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SCARF2(human-gc-opt)-EF1a-CopGFP-Puro
Alias: NM_153334.7;NSR1; SREC-II; SREC2; SRECRP-1; VDEGS
Gene lenght: 2616bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SCARF2-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-SCARF2-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SCARF2-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCATGGAAGG TGCTGGTCCA AGAGGTGCTG GTCCAGCTAG 2341 AAGAAGAGGT GCTGGAGGGC CTCCATCTCC GTTGTTGCCA AGTCTGCTGC TCCTGCTGCT 2401 CTTGTGGATG CTCCCCGATA CTGTGGCACC ACAAGAACTG AACCCAAGGG GACGGAATGT 2461 GTGTAGGGCT CCTGGGAGTC AGGTACCTAC CTGTTGTGCC GGGTGGAGAC AGCAAGGTGA 2521 CGAATGTGGC ATCGCCGTCT GCGAAGGCAA CTCTACATGC TCAGAGAACG AGGTGTGTGT 2581 AAGACCTGGA GAATGTCGCT GCAGACACGG ATATTTTGGA GCTAATTGCG ACACTAAATG 2641 TCCTCGCCAG TTCTGGGGCC CAGACTGCAA AGAACTGTGT TCATGCCACC CTCACGGCCA 2701 GTGTGAGGAT GTGACAGGTC AGTGTACCTG TCACGCCAGA AGATGGGGTG CCCGGTGTGA 2761 GCACGCATGT CAGTGCCAGC ACGGAACTTG CCACCCAAGA AGCGGGGCTT GCAGGTGCGA 2821 ACCAGGTTGG TGGGGAGCTC AGTGTGCATC CGCTTGCTAC TGTAGTGCGA CCAGTAGGTG 2881 TGATCCACAA ACCGGCGCTT GTTTGTGCCA CGCTGGATGG TGGGGCAGAT CATGTAACAA 2941 TCAATGTGCA TGCAATTCTA GCCCCTGTGA GCAGCAATCA GGAAGGTGCC AATGTCGAGA 3001 GAGGACATTC GGGGCCCGGT GTGATAGGTA CTGCCAGTGT TTTCGCGGGC GATGTCATCC 3061 TGTTGATGGA ACATGCGCAT GCGAGCCTGG CTATAGGGGA AAGTACTGTA GAGAACCCTG 3121 CCCAGCTGGC TTTTACGGCC TGGGATGTAG AAGACGATGT GGTCAGTGCA AAGGGCAACA 3181 GCCATGTACC GTAGCAGAAG GCAGGTGCCT GACCTGTGAA CCAGGATGGA ATGGTACAAA 3241 GTGCGACCAG CCTTGTGCCA CAGGCTTCTA TGGGGAAGGC TGTTCTCACA GATGTCCGCC 3301 ATGTCGGGAT GGCCATGCTT GCAATCATGT TACCGGAAAG TGCACCCGCT GTAACGCCGG 3361 CTGGATTGGG GACAGGTGCG AAACCAAGTG CTCCAACGGA ACTTACGGGG AGGATTGCGC 3421 CTTTGTGTGT GCCGACTGCG GCTCCGGACA TTGCGACTTC CAGTCCGGGC GCTGTTTGTG 3481 TTCCCCAGGC GTACACGGGC CTCATTGCAA TGTGACGTGT CCTCCAGGAC TGCATGGGGC 3541 CGATTGTGCC CAGGCTTGTT CCTGTCACGA AGATACGTGT GACCCAGTTA CCGGGGCTTG 3601 CCACCTCGAA ACCAACCAGA GAAAAGGAGT GATGGGGGCT GGTGCCTTGC TGGTTCTGCT 3661 GGTGTGCCTG CTCCTGAGCC TGCTGGGATG CTGTTGTGCC TGTAGAGGGA AAGATCCCAC 3721 CAGAAGACCT AGGCCAAGAA GAGAACTTTC CCTCGGGCGA AAAAAGGCGC CACACAGACT 3781 GTGCGGCCGG TTCTCTAGGA TTAGCATGAA GCTGCCTAGA ATTCCCCTCA GACGACAAAA 3841 ACTGCCCAAG GTGGTGGTCG CACATCACGA TCTGGACAAT ACTCTGAATT GTAGCTTTCT 3901 TGAACCACCA TCAGGACTGG AGCAACCTAG TCCCTCTTGG TCTTCCCGCG CAAGCTTTTC 3961 TTCTTTTGAT ACAACTGATG AAGGGCCCGT CTATTGTGTG CCACACGAAG AGGCTCCCGC 4021 AGAAAGCAGA GATCCCGAAG TTCCTACTGT GCCAGCTGAA GCACCTGCTC CAAGCCCTGT 4081 GCCTCTTACT ACTCCCGCAT CTGCAGAAGA GGCAATTCCT CTGCCCGCTT CCTCAGACAG 4141 TGAAAGGTCA GCTTCTAGCG TGGAAGGACC AGGTGGGGCC CTCTATGCTA GGGTCGCAAG 4201 ACGCGAAGCC AGACCCGCTA GAGCAAGAGG GGAGATTGGC GGCCTGAGTC TGTCTCCTTC 4261 ACCTGAGCGC CGAAAACCCC CACCTCCTGA TCCTGCAACA AAACCCAAAG TGTCTTGGAT 4321 CCATGGCAAA CATAGTGCGG CTGCAGCAGG AAGGGCTCCT TCACCTCCAC CACCAGGCTC 4381 AGAAGCTGCA CCTAGTCCCT CCAAGCGCAA GCGCACCCCA TCAGACAAAA GCGCTCACAC 4441 TGTTGAGCAC GGCAGTCCCA GAACTAGAGA CCCTACGCCA CGACCACCTG GTCTGCCTGA 4501 AGAAGCCACA GCTCTTGCTG CTCCAAGCCC TCCTCGGGCT AGAGCAAGAG GGAGAGGGCC 4561 AGGACTTCTT GAGCCTACCG ATGCAGGGGG ACCTCCAAGA AGTGCGCCTG AAGCTGCTTC 4621 TATGCTGGCT GCTGAACTGC GCGGGAAAAC ACGGAGCTTG GGCAGGGCAG AAGTGGCCCT 4681 TGGAGCCCAA GGTCCTAGGG AAAAACCAGC CCCCCCACAA AAAGCTAAGA GATCAGTTCC 4741 TCCAGCAAGC CCAGCAAGAG CTCCACCTGC TACAGAAACT CCCGGTCCTG AGAAGGCCGC 4801 TACAGACCTG CCAGCTCCTG AAACCCCTCG AAAGAAGACG CCAATTCAGA AACCCCCACG 4861 GAAGAAGTCT AGAGAGGCTG CAGGCGAACT GGGTAGAGCT GGCGCACCAA CACTGTAGgg 4921 atccgcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat 4981 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa 5041 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 5101 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 5161 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc 5221 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg 5281 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc 5341 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga 5401 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca 5461 agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc ggcctgcccg 5521 ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg 5581 gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag 5641 gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact 5701 tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct 5761 acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct 5821 accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg 5881 aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc 5941 accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg 6001 gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca 6061 gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca 6121 acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc gccttcgcca 6181 gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg 6241 gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc 6301 ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg 6361 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg 6421 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 6481 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg 6541 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 6601 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg 6661 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg 6721 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 6781 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 6841 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct 6901 gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 6961 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 7021 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 7081 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 7141 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 7201 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 7261 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 7321 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 7381 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 7441 tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt 7501 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 7561 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt 7621 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 7681 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 7741 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 7801 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 7861 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 7921 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 7981 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca 8041 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 8101 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga 8161 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 8221 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 8281 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 8341 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 8401 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 8461 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 8521 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 8581 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 8641 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 8701 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 8761 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 8821 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 8881 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 8941 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 9001 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 9061 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 9121 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 9181 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 9241 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 9301 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 9361 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 9421 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 9481 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 9541 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 9601 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 9661 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 9721 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 9781 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 9841 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 9901 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 9961 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 10021 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 10081 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 10141 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 10201 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 10261 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 10321 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 10381 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 10441 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 10501 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 10561 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 10621 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 10681 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 10741 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 10801 caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.