pLV2-CMV-CHSY3-3×FLAG-Puro pla

pLV2-CMV-CHSY3-3×FLAG-Puro plasmid

PVT90564

pLV2-CMV-CHSY3-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-CHSY3(human)-3×FLAG-Puro
Alias: NM_175856.5;CHSY2; CSS3;
Gene lenght: 2646bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-CHSY3-3×FLAG-Puro plasmid Description

pLV2-CMV-CHSY3-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-CHSY3-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggctgt gcgctctcgc cgcccgtgga tgagcgtggc 2881 attagggctg gtgctgggct tcaccgccgc gtcctggctc atcgccccca gggtggcgga 2941 gctgagcgag aggaagagac gtggctccag cctctgctcc tactacggtc gctctgctgc 3001 tggcccccgc gccggcgctc agcagccgct cccccagccc cagtcccgac cacggcagga 3061 gcagtcgccg ccccccgcgc gccaggatct ccaggggcca ccgctgcccg aggcagcacc 3121 cgggatcacc agttttcgaa gcagcccctg gcagcagcca cctccgctgc agcagcggcg 3181 gcgaggacgc gagcctgagg gcgcgacggg gcttcccggt gctccagcgg ccgaggggga 3241 gcccgaggag gaggacgggg gcgcggctgg gcagcggaga gacggccggc cggggagtag 3301 ccacaacggc agcggggacg ggggcgctgc cgccccgagc gcccgacccc gggacttcct 3361 gtacgtgggg gtgatgaccg cgcagaagta cctgggcagc cgcgcgctgg ccgcgcagcg 3421 gacctgggcg cgtttcatcc cgggccgcgt ggagttcttt tccagccagc agccccccaa 3481 cgccggccag cccccgccac ccctgcctgt catcgcgcta ccgggtgtgg acgactccta 3541 tcctccccag aaaaagtcct tcatgatgat caagtacatg cacgaccact acctggacaa 3601 gtatgagtgg ttcatgcgcg ccgacgacga tgtctacatc aaaggtgata aattagaaga 3661 gtttcttaga tcgctaaaca gcagtaagcc tctctacctg ggacagactg gcctggggaa 3721 tattgaagag cttggaaagc tgggactgga gcctggggaa aacttctgta tgggaggacc 3781 tggcatgatc tttagccgag aagttctcag gaggatggtg ccacatattg gtgaatgcct 3841 tagagaaatg tacacgactc atgaggatgt ggaagtagga agatgcgttc gccgttttgg 3901 tgggactcag tgtgtctggt cttacgagat gcaacaactg ttccatgaaa attatgaaca 3961 caatcggaag ggttacatcc aagaccttca caatagcaaa atccatgcag ccataacact 4021 tcatcccaac aaaaggcctg cataccaata caggctgcat aattacatgc tcagccgcaa 4081 aatttctgaa cttcgctacc gcaccatcca gctccacagg gaaagtgccc tgatgagcaa 4141 gctcagtaac acagaagtga gcaaagagga ccagcagctg ggagtgatac cttctttcaa 4201 ccacttccag cctcgggaga gaaatgaagt gatagaatgg gagttcctga cagggaagct 4261 tctatactca gcagctgaga accagccccc tcgacagagc ctcagtagca ttttaagaac 4321 agcactggat gataccgtcc tacaggtgat ggagatgatc aatgagaatg ccaagagcag 4381 aggacggctc attgacttca aggaaattca gtatggctac cgcagagtta accccatgca 4441 cggggtggag tacattttgg atttactcct tttatacaaa agacacaagg gaaggaaact 4501 gactgtgcca gtgagacgtc atgcctatct tcagcagttg ttcagcaagc ctttcttcag 4561 agagaccgaa gagctagatg tcaacagtct tgtggagagt attaacagtg aaactcagtc 4621 attctccttt atatctaatt ctttaaagat attatcttct tttcaaggtg ccaaagaaat 4681 gggagggcac aatgaaaaga aagtacacat tctcgttcct ctcatcggaa ggtatgacat 4741 tttcttgaga ttcatggaga actttgaaaa catgtgtctt atcccaaagc agaatgtaaa 4801 gttggtcatt atccttttca gtagggattc tggccaagac tccagcaagc atattgagct 4861 gataaaaggg taccagaaca agtaccccaa agcagaaatg accctgatcc caatgaaggg 4921 agagttttcc agaggtcttg gtcttgaaat ggcttctgcc cagtttgaca atgacacttt 4981 gctgctattt tgtgatgttg acttgatctt cagagaagat tttctccaac gatgtagaga 5041 caatacaatt cagggacaac aggtgtacta tcccatcatc tttagccagt atgacccaaa 5101 ggtaacaaac gggggaaatc ctcccactga tgattacttc atattctcaa aaaagactgg 5161 attttggaga gactatggat atggcatcac ctgtatttac aaaagtgatc ttctaggtgc 5221 aggtggattt gatacctcaa tacaaggctg gggactagaa gatgtagatc tctacaataa 5281 agtcattcta tctggcttaa ggccattcag aagccaagaa gtaggagtgg tgcatatttt 5341 ccatccagtt cattgtgatc ctaacttgga ccctaagcag tataagatgt gcttaggatc 5401 caaggcaagt actttcgcct caaccatgca actggctgaa ctctggcttg aaaaacattt 5461 aggtgtcagg tacaatcgaa ctctctccGG TGGAGGCgga tccGACTACA AAGACCATGA 5521 CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGt agtctagata 5581 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 5641 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 5701 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 5761 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 5821 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 5881 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 5941 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 6001 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 6061 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac 6121 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 6181 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 6241 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 6301 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 6361 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 6421 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 6481 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 6541 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 6601 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 6661 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa 6721 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 6781 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 6841 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 6901 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 6961 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 7021 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 7081 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 7141 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 7201 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 7261 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa 7321 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 7381 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 7441 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 7501 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 7561 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca 7621 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat 7681 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat 7741 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt 7801 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc 7861 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag 7921 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg 7981 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 8041 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 8101 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 8161 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 8221 gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg taatcatggt 8281 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 8341 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 8401 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 8461 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 8521 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 8581 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 8641 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 8701 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 8761 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 8821 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 8881 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 8941 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 9001 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 9061 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 9121 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 9181 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 9241 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 9301 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 9361 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 9421 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 9481 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 9541 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 9601 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 9661 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 9721 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 9781 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 9841 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 9901 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 9961 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 10021 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 10081 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 10141 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 10201 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 10261 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 10321 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 10381 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 10441 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 10501 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 10561 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc 10621 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa 10681 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact 10741 acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag 10801 t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.